Nonstructural protein 5A
Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins
... (HCV) nonstructural protein 5A (NS5A) exhibits a preference for G/U-rich RNA in ...the protein includes the amino-terminal domain I as well as the subsequent linker that separates domains I ...
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Inhibition of Intrahepatic Gamma Interferon Production by Hepatitis C Virus Nonstructural Protein 5A in Transgenic Mice
... HCV nonstructural protein 5A (NS5A) impairs tumor necrosis factor alpha (TNF- ␣ )-mediated ...NS5A protein in transgenic mouse (NS5A-Tg) liver when mice were challenged with an unrelated ...
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Characterization of a Threonine-Rich Cluster in Hepatitis C Virus Nonstructural Protein 5A and Its Contribution to Hyperphosphorylation
... (HCV) nonstructural protein 5A (NS5A) is a phospho- protein with key functions in regulating viral RNA replication and ...phosphorylated protein species but rather a population of ...
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Role for TBC1D20 and Rab1 in Hepatitis C Virus Replication via Interaction with Lipid Droplet-Bound Nonstructural Protein 5A
... Replication and assembly of hepatitis C virus (HCV) depend on the host’s secretory and lipid-biosynthetic machinery. Viral rep- lication occurs on endoplasmic reticulum (ER)-derived modified membranes, while viral ...
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Mutational Analysis of Hepatitis C Virus Nonstructural Protein 5A: Potential Role of Differential Phosphorylation in RNA Replication and Identification of a Genetically Flexible Domain
... Nonstructural protein 5A of the hepatitis C virus (HCV) is a highly phosphorylated molecule implicated in multiple interactions with the host cell and most likely involved in RNA ...
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A Conserved Tandem Cyclophilin-Binding Site in Hepatitis C Virus Nonstructural Protein 5A Regulates Alisporivir Susceptibility
... Cyclophilin A (CyPA) and its peptidyl-prolyl isomerase (PPIase) activity play an essential role in hepatitis C virus (HCV) replica- tion, and mounting evidence indicates that nonstructural protein 5A ...
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Conserved Determinants for Membrane Association of Nonstructural Protein 5A from Hepatitis C Virus and Related Viruses
... Nonstructural protein 5A (NS5A) is a membrane-associated essential component of the hepatitis C virus (HCV) replication ...specific protein-protein interactions required for the ...
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Nonstructural Protein 5A of Hepatitis C Virus Inhibits the Function of Karyopherin β3
... -treated fragment of the PCR product, and the BamHI-EcoRV fragment of pSK-karyopherin 3 were ligated to generate plas- mid pCMV/myc-karyopherin 3. The plasmid pCMV/HA-NS5A, encoding HCV NS5A and a hemagglutinin (HA) ...
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Nonstructural Protein 5A (NS5A) and Human Replication Protein A Increase the Processivity of Hepatitis C Virus NS5B Polymerase Activity In Vitro
... Protein-protein interactions within the replicase. The inter- actions between replicative DNA polymerases and helicases are known from studies in the T7 and E. coli DNA replication systems (67, 78). We have ...
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The C-Terminal α-Helix Domain of Apolipoprotein E Is Required for Interaction with Nonstructural Protein 5A and Assembly of Hepatitis C Virus
... Mutations disrupting the apoE-NS5A interaction suppressed HCV production. To determine the effects of apoE mutations on HCV assembly, all deletion mutations were introduced into apoE3 cDNA vector pCMV-XL5-mApoE3, which ...
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Hepatitis C Virus Nonstructural Protein 5A Modulates the Toll-Like Receptor-MyD88-Dependent Signaling Pathway in Macrophage Cell Lines
... A46R protein contains a Toll/IL-1 receptor domain that inter- acts with multiple Toll/IL-1 receptor-containing adaptor mol- ecules, thereby inhibiting the activation of NF- B and IRF3 ...
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Functional Characterization of Bovine Viral Diarrhea Virus Nonstructural Protein 5A by Reverse Genetic Analysis and Live Cell Imaging
... C-terminal half of the NS5A protein to determine its functional importance in the pestiviral life cycle. The minimal set of viral proteins required for RNA replication of pestiviruses is the NS3 to NS5B region of ...
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Hepatitis C virus nonstructural protein 5A perturbs lipid metabolism by modulating AMPK/SREBP-1c signaling
... To further confirm our results and understand whether increased p-AMPK attenuates NS5A-induced lipogen- esis, Hepa1–6 and HepG2 cells were co-transfected with NS5A-, AMPKα1- and AMPKα2-expressing plasmids. As expected, ...
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Interaction of Hepatitis C Virus Nonstructural Protein 5A with Core Protein Is Critical for the Production of Infectious Virus Particles
... serine/threonine protein kinases that block NS5A hyperphosphorylation facili- tate replication of a non-culture-adapted replicon (3, ...membrane protein-associated protein subtype A, a cel- lular ...
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Sequential S232/S235/S238 Phosphorylation of the Hepatitis C Virus Nonstructural Protein 5A
... biochemical characterization (8), this intrinsically disordered LCS I region together with the domain I region of NS5A constitutes an RNA-binding groove in an NS5A dimer (7). Previously, phosphorylation of S146 at the ...
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Efficient Rescue of Hepatitis C Virus RNA Replication by trans-Complementation with Nonstructural Protein 5A
... -replication assay is shown in Fig. 5A. Forty-eight hours after transfection, the luciferase activity observed with the luc-2197 ⫹ 2201-T mutant was below the detection limit. Cotransfection with the defective ...
Nonstructural Protein 5A Impairs DNA Damage Repair: Implications for Hepatitis C Virus-Mediated Hepatocarcinogenesis
... interaction. For this purpose, HEK293T cells were cotransfected with Flag-tagged RAD51AP1, V5-tagged UAF1, and Myc-tagged NS5A. At 48 h posttransfection, cell lysates were immunoprecipitated with an anti-Flag antibody, ...
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Approaches to hepatitis C treatment and cure using NS5A inhibitors
... NS5A, nonstructural protein 5A; PI4K, phosphatidylinositol 4-kinase; PIP4, phosphatid- ylinositol phosphate 4; pdb, protein data bank; RNA, ribonucleic acid; ...
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Regulation of Hepatitis C Virus Replication by Nuclear Translocation of Nonstructural 5A Protein and Transcriptional Activation of Host Genes
... (HCV) nonstructural protein 5A (NS5A) is involved in regulating viral replication through its direct interac- tion with the HCV RNA-dependent RNA ...
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Subversion of Cell Signaling Pathways by Hepatitis C Virus Nonstructural 5A Protein via Interaction with Grb2 and P85 Phosphatidylinositol 3-Kinase
... NS5A protein purified from insect cells was used for GST pulldown assays with GST-Grb2 (lane 2), GST-Gab1 (lane 3), GST-p85 PI3K (lane 4), GST–C-terminal SH2 domain of p85 (lane 5), GST–N-terminal SH2 domain of ...
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