normal rabbit serum
Effect of heat on flocculating antibodies of rabbit antisera
... On the other hand, diluting the antisera in protein solutions (normal rabbit serum, rabbit serum albumin and pseudoglobulin, human serum albumin) enhances the destructive effect of heati[r] ...
18
Isolation of monoclonal antibodies against avian oncornaviral protein p19.
... Asc,, Ascites fluid 5 td; NS-1, culture fluid 5 ,uI serving as a negative control for the hybridoma supernatants; Rapl9, monospecific rabbit anti-p19 antibodies; NRS, normal rabbit serum[r] ...
5
Carumonam enhances reactivity of Escherichia coli with mono and polyclonal antisera to rough Escherichia coli J5
... coli 0111 grown without carumonam or in the presence of one-half the MIC of carumonam, with various antisera NRS, normal rabbit serum, with or without absorption.. Symbols: M, nonabsorbe[r] ...
5
Detection and quantitation of toxic shock syndrome toxin 1 in vitro and in vivo by noncompetitive enzyme linked immunosorbent assay
... Of 60 isolates negative for TSST-1 production as tested by immunodiffusion, 42 would be falsely positive by ELISA if culture supernatants were not pretreated with 10% normal rabbit serum[r] ...
6
Forensic Immunology
... In order to be able to make positively the diagnosis of human origin of unknown material, the controls as outlined above are needed, viz: Normal rabbit serum: this is used to test if the[r] ...
19
Functional Profile of the Isolated Uremic Nephron: EVIDENCE OF PROXIMAL TUBULAR “MEMORY” IN EXPERIMENTAL RENAL DISEASE
... FIGURE 1 Net water flux per millimeter tubule length Jv in superficial proximal convoluted tubules from normal, remnant, and embolized kidneys perfused in a bath of normal rabbit serum..[r] ...
9
Inhibition of transcription by immunoglobulins directed against the ribonucleoprotein of homotypic and heterotypic vesicular stomatitis viruses.
... Furthermore, an following indirect raclioimmunoprecipitation by anti- Ig fraction prepared from normal rabbit serum RNP1,d and antiwhodle VS1,,d virion sera of soluble had no significant[r] ...
10
Principles, problems, and strategies in the use of antigenic mixtures for the enzyme linked immunosorbent assay
... Coating with 1:100 normal rabbit serum gave similar results when tested with conjugated rabbit antiserum 1:1,000 to BSA or human IgG; coated type B plates showed significantly higher opt[r] ...
11
The Physiological Role of Thyrotropin Releasing Hormone in the Regulation of Thyroid Stimulating Hormone and Prolactin Secretion in the Rat
... catheter. Serum samples were obtained before and at frequent intervals ...thereafter. Serum TSH concentrations in normal, thyroidectomized, cold-exposed, and proestrus rats were not depressed in ...
9
Characterization of bovine immunodeficiency virus rev cDNAs and identification and subcellular localization of the Rev protein.
... A RIP of 32P-labeled proteins from BIV127-infected BLAC-20 cells, using rabbit anti-Rev peptide serum lane 1 or normal rabbit serum lane 2.. B Immunoblot developed with rabbit anti-Rev p[r] ...
11
Porcine parvovirus: virus purification and structural and antigenic properties of virion polypeptides.
... Various antisera used in the present study Antiserum Abbreviation Method of production/source Normal pig serum Adult pig aPPV Fetal pig otPPV Normal rabbit serum Rabbit a intact PPV Rabb[r] ...
13
The modification of biophysical and endotoxic properties of bacterial lipopolysaccharides by serum
... Normal rabbit serum reduces the buoyant density of lipopolysaccharide (LPS) from Escherichia coli 0111:B4 (d = 1.44 g/cm3) and Salmonella minnesota R595 (d = 1.38 g/cm3) to a value less than g/cm3. ...
13
INVITRO STUDY OF ASPERGILLOSIS AND THE EFFECT OF SELECTED TRADITIONAL PLANTS AGAINST THE PATHOGENIC FUNGAL ORGANISM OF ASPERGILLUS FUMIGATES
... generally produce many secondary metabolites which constitute an important source of microbicides, pesticides and many pharmaceutical drugs. Antimicrobial activity of plant essential oils also used recently (Thenmozhi et ...
10
MIP 1alpha as a critical macrophage chemoattractant in murine wound repair
... quently, many studies have provided additional information about the multifunctional role of macrophages within wounds. For example, macrophages have been shown to affect the ex- tracellular matrix composition after ...
7
Antibody Responses to Escherichia coli O157 and Other Lipopolysaccharides in Healthy Children and Adults
... of serum antibodies is important for the host in the elimination of microorganisms from muco- sal ...of rabbit antisera prepared against the O7, O116, and O157 LPSs, suggest that the low incidence of ...
5
Isolation and comparison of murine leukemia virus-related glycoproteins from AKR and New Zealand mice.
... To accentuate differences in antigen binding of the various gp7O preparations, absorbed antisera were prepared as follows: 100 pl of G145 serum was treated with 4 ml of i normal rabbit s[r] ...
7
MONOCLONAL ANTIBODIES TO HUMAN ALPHA-FETOPROTEIN
... raphy, Sephadex G-200, blue sepharose and affinity chro matography. In the present study, since a complete puri fication of the antigen was not necessary for production of mAbs against AFP, the semi-purification of AFP ...
6
Effects of T 2 mycotoxin ingestion on selected resistance mechanisms and on experimentally induced aspergillosis in rabbits
... EFFECTS OF T-2 MYCOTOXIN INGESTION ON PHAGOCYTOSIS OF ASPERGILLUS FUMIGATUS CONIDIA BY RABBIT ALVEOLAR MACROPHAGES AND ON HEMATOLOGIC, SERUM BIOCHEMICAL, AND PATHOLOGIC CHANGES IN RABBIT[r] ...
152
Expression of Aleutian mink disease parvovirus capsid proteins in defined segments: localization of immunoreactive sites and neutralizing epitopes to specific regions.
... The capsid proteins of the ADV-G isolate of Aleutian mink disease parvovirus (ADV) were expressed in 10 nonoverlapping segments as fusions with maltose-binding protein in pMAL-C2 (pVP1, pVP2a through pVP2i). The ...
10
The experimental generation of antibodies to alpha melanocyte stimulating hormone and adrenocorticotropic hormone
... 1 Abbreviations: MSH, melanocyte stimulating hormone; ACTH, adrenocorticotropic hormone; RSA, rabbit serum albumin; anti-ACTH, serum from rabbits that have received injections of rabbit [r] ...
8