nuclease digestion
Comparison of nuclease digestion of polyoma virus nucleoprotein complex and mouse chromatin.
... Lengths of DNA fragments obtained from digestion ofpolyoma and mouse chromatin with micrococcal nuclease" DNA fragment Length of DNA fragment base pairs Polyoma virions Mouse nuclei 0.3b[r] ...
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Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion.
... Virion assembly intermediates with different degrees of virion maturation showed intermediate resistance, and three products were found: nucleosomal DNA fragments, representing the fract[r] ...
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Hepatitis B Virus Covalently Closed Circular DNA Formation in Immortalized Mouse Hepatocytes Associated with Nucleocapsid Destabilization
... and nuclease digestion, sedimentation on sucrose gradi- ents, and migration on agarose gels) ...and nuclease digestion and their sedimentation and migration profiles on the sucrose gradients ...
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RNase footprinting demonstrates antigenomic hepatitis delta virus ribozyme structural rearrangement as a result of self cleavage reaction
... similar nuclease digestion profiles for the original molecule (Figures 2a and 2f, 3a and 3f, 4a and ...The nuclease cleavage patterns are not the same, but the major features are ...
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Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples
... the digestion of human DNA containing high numbers of repetitive (low entropy) sequences and important for maximizing the amount of quality reads to increase sen- ...sitivity. Nuclease treatment enriched ...
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Nucleosome-like structural subunits of intranuclear parental adenovirus type 2 DNA.
... Recovery of 32P-labeled adenovirus 2 DNA and viral transcriptional activity in nuclei prepared for micrococcal nuclease digestion iii, as compared to crude nuclear preparations i, iia Vi[r] ...
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Psoralen-cross-linking study of the organization of intracellular adenovirus nucleoprotein complexes.
... Contrary to the results of nuclease digestion studies, analyses of viral NPCs extracted from adenovirus type 2 Ad2-infected HeLa cell nuclei at late times after infection by electron mic[r] ...
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cis-Acting RNA packaging locus in the 115-nucleotide direct repeat of Rous sarcoma virus.
... Figure 4, lane B, shows the Si nuclease digestion products after hybridization of the same probe to virion RNA harvested 48 h posttransfection from the culture medium of the same plate o[r] ...
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Intracellular DNA of the parvovirus minute virus of mice is organized in a minichromosome structure.
... Electrophoresis of DNA fragments pro- complexes can be leached from infected cell duced by micrococcal nuclease digestion of the DPC nuclei in the presence of hypotonic buffer 11, presen[r] ...
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Epigenetic Status of an Adenovirus Type 12 Transgenome upon Long-Term Cultivation in Hamster Cells
... Cell lines T637 and TR12 offer the advantage of analyzing foreign DNA integrates of about 15 copies and 1 copy plus a 3.9-kbp fragment of Ad12 DNA, respectively, at the same chromosomal site and at the same time after ...
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Capsid Phosphorylation State and Hepadnavirus Virion Secretion
... When the virion DNA signal was normalized to intracellular viral DNA extracted with nuclease digestion, the virion secretion efficiency, defined by the ratio of extracellular RC DNA V comp[r] ...
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Labelling of live cells using fluorescent aptamers: binding reversal with DNA nucleases
... A reversible cell labelling method has been developed for non-destructive and non-invasive cell labelling and purification. Our method uses high affinity single strand DNA (ssDNA) aptamers against surface exposed target ...
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Characterization of mRNAs that map in the BglII N fragment of the herpes simplex virus type 2 genome.
... Hybridization and S1 nuclease digestion of p and y cytoplasmic RNA to pNBal DNA 0.601 to 0.620 map units 5' end labeled at the BglII site position 0.620 protected a DNA band of 0.55 kb i[r] ...
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Cleavage of phi X174 single-stranded DNA by gene A protein and formation of a tight protein-DNA complex.
... Less than 0.5% and 0.1% of the total 32p label remained resistant to DNase I and micrococcal nuclease digestion, respectively, measured as trichloroacetic acid-precipitable The introduct[r] ...
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Transcription of overlapping sets of RNAs from the genome of Autographa californica nuclear polyhedrosis virus: a novel method for mapping RNAs.
... After S1 nuclease digestion, resistant hybrids were separated by two-dimnensional electrophoresis on 1.5% neutral N and alkaline A agarose gels, transferred to nitrocellulose filters, an[r] ...
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The covalently closed duplex form of the hepadnavirus genome exists in situ as a heterogeneous population of viral minichromosomes.
... micrococcal nuclease digestion, and DNA superhelicity ...micrococcal nuclease, a ladder of viral DNA fragments that exhibits a 150-bp repeat is ...
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The Distribution and Substrate Specificity of Extracellular Nuclease Activity in Marine Fungi
... It is known that a large group of fungi and filamentous bacteria generate and disseminate spores for implement of survival program in stressful environmental conditions. These events call forth increase of ...
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Biochemical and Biological in vivo Functions of Dna2p in Saccharomyces cerevisiae
... Several known helicases yeast SCSI, the Werner syndrome helicase, and the bacterial RecE,C,D enzymel are associated with nuclease activities, although none of the known helicase/nuclease[r] ...
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POTENTIAL ROLE OF ANTISENSE OLIGONUCLEOTIDES IN REGULATION OF GENE EXPRESSION TO TARGET CANCER: A STEP FORWARD
... These are formed when one of the non-bridging oxygen atoms in the phosphate group is replaced by a sulphur atom to form phosphorothioates, or a methyl group to form methyl phosphonates respectively. When compared to ...
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Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization.
... mutants lacking addA or addB genes lack detectable ATP-dependent nuclease activity, and the cloned 31.. pylori addAB genes restore both nuclease and helicase activities to an E.[r] ...
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