Nucleic Acid Probes
Functional nucleic acid probes and uses thereof
... functional nucleic acid probes, and methods of using functional nucleic acid probes, for binding a target to carry out a desired ...The probes have at least one functional ...
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Development and Evaluation of Novel Real Time Reverse Transcription PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human Pathogenic Coronaviruses
... 35. Thanh TT, Pawestri HA, Ngoc NM, Hien VM, Syahrial H, Trung NV, van Doorn RH, Wertheim HF, Chau NV, Ha do Q, Farrar JJ, Hien TT, Sedyaningsih ER, de Jong MD. 2010. A real-time RT-PCR for detection of clade 1 and 2 ...
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Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Mycobacterium Cultures
... Tuberculosis (TB) is caused by infection with species of the Mycobacterium tuberculosis complex (MTC), in particular, M. tuberculosis, and is globally the most important cause of death from a single pathogen (11, 12). ...
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Rapid Identification of Staphylococcus aureus Directly from Blood Cultures by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes
... S. aureus PNA FISH assay performance. The PNA probe targets the same species-specific region of the 16S rRNA of S. aureus that previously published DNA probes target (5, 11); however, the PNA probe is shorter than ...
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Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes for Rapid Identification of Candida albicans Directly from Blood Culture Bottles
... The C. albicans-specific PNA probe showed a very high degree of specificity not only when it was used to test clinical isolates but also when it was further challenged by use of strains from the C. albicans clade. These ...
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Identification of Fusarium Species in Formalin Fixed and Paraffin Embedded Sections by In Situ Hybridization Using Peptide Nucleic Acid Probes
... PNA probes. The design of the PNA probes for Fusarium spp. (N terminus- GAT GAT CAA CCA AGC CCA) and panfungal species (N terminus-TAC TTG TGC GCT ATC GGT) was derived from a comparison of 28S rRNA genes in ...
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Design and Evaluation of Peptide Nucleic Acid Probes for Specific Identification of Candida albicans
... Peptide nucleic acid (PNA) probes are synthetic DNA mimics with improved performance characteristics compared to DNA ...DNA-based probes targeting the small (18S) ribosomal sub- unit ...
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Simultaneous Detection and Differentiation of Human Rhino and Enteroviruses in Clinical Specimens by Real Time PCR with Locked Nucleic Acid Probes
... final reaction mixture consisted of QuantiTect Probe master mix (Qia- gen), 600 nM primers ENRI3 ⫹ and ENRI4 ⫺ , 100 nM each of the probes RIp1, RIp2, ENp1, and ENp2 (Table 1), and 0.3 M BOXTO (TATAA Biocenter, ...
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Development of a streamlined, selective enrichment culture, one tube (RT )PCR enzyme hybridization assay to detect bacterial fish pathogens in covertly infected farmed salmonids
... Therefore while nucleic acid probes are useful for the diagnosis of fish pathogens that are present in numbers sufficient to be found in situ or by blotting methods, for applications whe[r] ...
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Detection of DNA and RNA virus genomes in organ systems of whole mice: patterns of mouse organ infection by polyomavirus.
... The use of hybridization of virus-specific nucleic acid probes with frozen sections of whole mice transferred to nitrocellulose has allowed us to quantitate viral genome accumulation in [r] ...
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Molecular probes for diagnosis of fungal infections
... specific nucleic acid probes which target the large subunit rRNA genes from Aspergillus flavus, Aspergillus fumigatus, Aspergillus glaucus, Aspergillus niger, Aspergillus terreus, Blastomyces ...
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Review Article Molecular Beacons in Biomedical Detection and Clinical Diagnosis
... single-stranded nucleic acid probes composed of three different functional domains: a stem, a loop, and a fluorophore/quencher pair (Figure 1) ...
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Molecular epidemiology of Escherichia coli O157:H7 by restriction fragment length polymorphism using Shiga like toxin genes
... of nucleic acid probes from Shiga-like toxin (SLT) I and II structural genes was used to generate restriction fragment length polymorphism (RFLP) patterns of SLTEC strains, (SLT-RFLP patterns) ...
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Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid
... of nucleic acid MBs have been de- veloped which displays increased thermal stabilities and specific hybridization ability when hybridized with complementary DNA/RNA ...detect nucleic acid, ...
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Development of nucleic acid vaccines: use of self-amplifying RNA in lipid nanoparticles
... Vaccines based on nucleic acids (both DNA and RNA) have been broadly investigated for several years, but have not yet resulted in a commercial product for human use. Self-amplifying RNA vaccines have been shown to ...
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Nucleic acid chaperons: a theory of an RNA-assisted protein folding
... amino acid replacement, and that if such changes affect protein folding, this would have restrained such nucleotide replacements ...amino acid co-locations is less than 200 (20 × 20/2), and the pos- sible ...
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Colorimetric Nucleic Acid Testing Assay for RNA Virus Detection Based on Circle to Circle Amplification of Padlock Probes
... on nucleic acid testing, such as PCR, can fulfill the requirements of being sensitive and ...padlock probes (16) and colorimetric readout, which can potentially overcome the drawbacks of PCR-based ...
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On nucleic acid photochemistry
... In order to locate the position of thymine and thymine photodimer on the strip of paper, a strip of paper containing just carrier thymine and thymine dimer was chromatographed adjacent t[r] ...
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Week16-01 (Lec) Micro 20 (Intro to Viruses).pptx
... wall; no uncoating of nucleic acid Whole virus is engulfed and uncoated, or virus surface fuses with cell membrane; nucleic acid is released. Synthesis and[r] ...
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Purification of Nucleic Acids by Hybridization to Affinity Tagged PNA Probes
... capture probes, the hybridization conditions can be adjusted so as to unwind nucleic acid structures (thereby making them accessible for hybridization) whilst not affecting PNA binding (11, ...
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