O-GlcNAc/P
Investigating the Role of O-GlcNAc Glycosylation in Neurodegeneration
... Whole brains were homogenized in 600 uL tissue homogenization buffer (THB) with PIC and PMSF using glass teflon douncer (about 10 dounces). 50mM Tris HCl pH 8, 274 mM NaCl, 5 mM KCl, 2 mM EDTA, 2 mM EGTA, 30 mM NaF, 1x ...
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Metabolic inhibitors of O GlcNAc transferase (OGT) that act in vivo implicate decreased O GlcNAc levels in leptin mediated nutrient sensing
... Schart, A. Timper, W. Schrimpf, C. R. Hauck, A. Zumbusch, V. Wittmann, Angew. Chem. Int. Ed. 2016, 55, 2262 – 2266; Angew. Chem. 2016, 128, 2303 – 2308; e) C. M. Speakman, T. C. Domke, W. Wongpaiboonwattana, K. Sanders, ...
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O-GlcNAc modification of Sox2 regulates self-renewal in pancreatic cancer by promoting its stability
... Figure 4. Transcriptional regulation of self-renewal in pancreatic cancer is dependent on O-GlcNAc modification of Sox2. Immunoprecipitation with anti-DDK antibody followed by western blotting with ...
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O-GlcNAc regulation of autophagy and α-synuclein homeostasis; implications for Parkinson’s disease
... increased p-MTOR (Ser2448)/total MTOR ...overall O-GlcNAcylated protein levels (p = ...the O-GlcNAc moiety to proteins, O-GlcNAc transferase ...
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Vol 72, No 2 (2020)
... Diabetes was induced by intraperitoneal injection (i.p.) of 40 mg/kg STZ in 0.1 M sodium citrate buffer, pH 4.5 (MP Biomedicals, Solon, OH, USA), to male albino Wistar rats for five consecutive days. Blood glucose was ...
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Inhibition of O-GlcNAc transferase activity reprograms prostate cancer cell metabolism
... Metabolic networks are highly connected and complex, but a single enzyme, O-GlcNAc transferase (OGT) can sense the availability of metabolites and also modify target proteins. We show that inhibition of OGT ...
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High OGT activity is essential for MYC-driven proliferation of prostate cancer cells
... chromatin O-GlcNAc and identify transcription factors that coordinate with OGT to promote proliferation of prostate cancer ...of O-GlcNAc chromatin mark. O-GlcNAc ChIP-seq ...
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Exercise and diabetes have opposite effects on the assembly and O-GlcNAc modification of the mSin3A/HDAC1/2 complex in the heart
... Although we did not measure the structural or hemo- dynamic effects of the exercise protocol in db hearts, previ- ous work has shown that the db heart shows similar cardiomyopathy to humans with type 2 diabetes [68,69], ...
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Discovery and Development of Small Molecule Modulators for the Sulfation of Glycosaminoglycans and Studying the Role of O GlcNAc on CREB through Semisynthesis
... Synthesis of O-mesitylenesulfonylhydroxylamine (MSH). Caution, MSH can detonate if crystallized improperly. 7.1 g (24.8 mmol) of 1 was added to 6.4 mL of p-dioxane and stirred at 0°C for 20 min. 2.9 mL of ...
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DYSREGULATION OF O-LINKED β-N-ACETYLGLUCOSAMINE (O-GLCNAC) CYCLING SUPPORTS TUMORIGENICITY OF CANCERS OF THE FEMALE REPRODUCTIVE TRACT, Nicole Jaskiewicz
... (Figure 4), despite the observation that K18 and β-actin expression were both diminished. Others have observed that α -tubulin and β -actin expression in colon cancer cells are unaffected by inhibition of OGT, yet the ...
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O-GlcNAcylation of master growth repressor DELLA by SECRET AGENT modulates multiple signaling pathways in Arabidopsis
... that O-GlcNAcylation of an RNA- binding protein (TaGRP2) plays a role in floral induction in response to vernalization in wheat (Xiao et ...of O-GlcNAcylated TaGRP2 by MS analysis and identification and ...
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Human linker histones: interplay between phosphorylation and O-β-GlcNAc to mediate chromatin structural modifications
... known. O- b -GlcNAc modification can occur on these Ser and Thr residues where kinases are involved in phosphory- lation as it is well known that kinases and OGT can compete for same site modification ...
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O-GlcNAc modification is associated with insulin sensitivity in the whole blood of healthy young adult males
... that O-GlcNAc modification can be used to discriminate between LH and HH in a young, healthy ...that O- GlcNAc analysis exhibits greater sensitivity and could potentially serve as a better ...
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PTM-ssMP: A Web Server for Predicting Different Types of Post-translational Modification Sites Using Novel Site-specific Modification Profile
... In this section, we adopt ten-fold cross-validation to evaluate the performance of ssMP in PTM site prediction based on benchmark dataset, and we assess the prediction models trained by both local sequences and ssMP are ...
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Glycomic Approaches to Study GlcNAcylation: Protein Identification, Site-mapping, and Site-specific O-GlcNAc Quantitation
... gels. O-GlcNAc was first discovered in experi- ments designed to enzymatically label terminal GlcNAc on lymphocytic proteins with β1,4-galactosyltransferase (GalT) and tritiated UDP-galactose ...
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The bacteriostatic spectrum and inhibitory mechanism of glycocin F, a bacteriocin from Lactobacillus plantarum KW30 : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology at Massey University, Palmerston North, New Zealand
... of GlcNAc, shown on plates as a crescent-shaped zone of inhibited bacterial growth between the square containing GccF and that containing ...whereby GlcNAc becomes ...
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Chitin Synthesis in Saccharomyces cerevisiae in Response to Supplementation of Growth Medium with Glucosamine and Cell Wall Stress
... Microarray chips used in these studies (GeneChip Yeast Genome S98 Array; Affymetrix) contained approximately 6,200 S. cerevisiae ORFs recognized by the Saccharomyces Ge- nome Database and an additional 600 putative ORFs ...
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Advances in understanding glycosyltransferases from a structural perspective
... An O-linked N-acetylglucosamine residue (O-GlcNAc) is added to serine or threonine residues (via a b-linkage) in a variety of nucleocytoplasmic proteins in ...the O-GlcNAc modification ...
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A Study of Mitochondrial Dynamics and Glycosylation Events in PC12 Cells and Neurons
... for O- GlcNAcylation of mitochondrial proteins meaning mOGT is redundant and indeed not expressed in many species (Trapannone et ...As GlcNAc is transferred to its target protein, UDP is released and acts ...
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O-GlcNAc transferase suppresses necroptosis and liver fibrosis
... increasing O-GlcNAcy- lation promotes cell survival under stress ...of O-GlcNAcylation, primary hepatocytes from WT mice were transfected with scrambled siRNA or OGT siRNA to assess whether acute deletion ...
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