O-GlcNAc transferase
Inhibition of O-GlcNAc transferase activity reprograms prostate cancer cell metabolism
... Metabolic networks are highly connected and complex, but a single enzyme, O-GlcNAc transferase (OGT) can sense the availability of metabolites and also modify target proteins. We show that inhibition ...
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O-GlcNAc transferase suppresses necroptosis and liver fibrosis
... that O-linked b-N-acetylglucosamine (O-GlcNAc) modification protects against hepatocyte necroptosis and initiation of liver ...Decreased O-GlcNAc levels were seen in patients with ...
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Original Article Increased expression of O-GlcNAc transferase (OGT) is a biomarker for poor prognosis and allows tumorigenesis and invasion in colon cancer
... Elevated O-GlcNAcylation by increased O-GlcNAc transferase (OGT) and/or decreasing O-GlcNAcase (OGA) levels is associated with cancer initiation, progression, invasion, and ...
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Identification of Secret Agent as the O-GlcNAc Transferase That Participates in Plum Pox Virus Infection
... that O-GlcNAc modification by SEC OGT is not essential for PPV viability in ...tion. O-GlcNAc modifications at the segment from amino ac- ids 1 through 39 of PPV CP are not essential, since ...
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Metabolic inhibitors of O GlcNAc transferase (OGT) that act in vivo implicate decreased O GlcNAc levels in leptin mediated nutrient sensing
... in O-GlcNAc levels by immunoblot analysis ...decreased O-GlcNAc and Sp1 levels to a minimum at 16 h in fat pad ...lowered GlcNAc-induced leptin secretion from 3T3- L1 ...
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Advances in understanding glycosyltransferases from a structural perspective
... the O-GlcNAc transferase and oligosaccharyltransferase, which are discussed further below, contain no obvious candidate catalytic base indicating these enzymes use an alternative ...
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A novel deconvolution method for modeling UDP-N-acetyl-D-glucosamine biosynthetic pathways based on 13C mass isotopologue profiles under non-steady-state conditions
... and O-linked glycosylation of proteins, which are important in regulating numerous cellular processes, such as protein targeting to organelles [14] and nutrient sensing ...With O-linked glycosylation, cyto- ...
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O-GlcNAc modification is associated with insulin sensitivity in the whole blood of healthy young adult males
... protein O-GlcNAc was examined. O-GlcNAc and O-GlcNAc transferase (OGT) levels were quantified by immunoblotting and compared to anthropometric measures: body mass index ...
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High OGT activity is essential for MYC-driven proliferation of prostate cancer cells
... OGA: O-GlcNAcase; OGT: O-GlcNAc transferase; pFa: para-formaldehyde; p-S2/5-Pol II: RNA polymerase II CTD repeat phospho serine 2/ serine 5; p-H3ser10: Phospho-Histone H3 Ser10; PUGNAc: ...
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Increased sugar uptake promotes oncogenesis via EPAC/RAP1 and O GlcNAc pathways
... and O-linked N-acetylglucosamine (O-GlcNAc) ...inhibits O-GlcNAc transferase–mediated (OGT-mediated) O-GlcNAcylation (39), also induced phenotypic reversion, although ...
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O-GlcNAc regulation of autophagy and α-synuclein homeostasis; implications for Parkinson’s disease
... In contrast, we observed that thiamet G increased p-MTOR (Ser2448)/total MTOR 2.5 fold after 24 h (Fig. 5a) and also at 7 days albeit to a lesser extent (Fig. 5b). We then tested the impact of rapamycin in the presence ...
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Human linker histones: interplay between phosphorylation and O-β-GlcNAc to mediate chromatin structural modifications
... the o-GlcNAc modification in mouse linker histone ...increased O-GlcNAc resulted in growth defects linked to delay in G2/M progression, altered mitotic phosphorylation, and cyclin ...of ...
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Exercise and diabetes have opposite effects on the assembly and O-GlcNAc modification of the mSin3A/HDAC1/2 complex in the heart
... by O-linked β-N- acetylglucosamine (O-GlcNAc). O-GlcNAc is a glucose derivative that post-translationally modifies serine/threo- nine residues ...[30,31]. O-GlcNAcylation ...
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O-GlcNAcylation of master growth repressor DELLA by SECRET AGENT modulates multiple signaling pathways in Arabidopsis
... and O-GlcNAcylation (Supplemental ...of O-GlcNAcy- lation in regulating GA signaling came from the identi- fication of a putative O-linked N-acetylglucosamine (O-GlcNAc) ...
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Discovery and Development of Small Molecule Modulators for the Sulfation of Glycosaminoglycans and Studying the Role of O GlcNAc on CREB through Semisynthesis
... Further verification of the reaction was performed through collision-induced dissociation mass spectrometry (CID-MS) and purification by wheat germ agglutinin (WGA) affinity chromatography to enrich for GlcNAcylated ...
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Glycomic Approaches to Study GlcNAcylation: Protein Identification, Site-mapping, and Site-specific O-GlcNAc Quantitation
... purify O-GlcNAcylated proteins with immunoaffinity chromatography, and then identify the bound proteins after elution ...with O-GlcNAc ...free GlcNAc in primary antibody solution to compete ...
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Glucosamine impedes transforming growth factor β1-mediated corneal fibroblast differentiation by targeting Krüppel-like factor 4
... of O-linked N-acetylglucosamine ( O -GlcNAc). O - GlcNAc is linked to serine or threonine residues in pro- teins through a reaction catalyzed by O-linked-β-N-acet- ...
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Vol 72, No 2 (2020)
... increased O-GlcNAcylation is directly linked to hyperglycemia-induced glucose toxicity and diabetic complications, through the enzymatic addi- tion of the N-acetyl glucosamine (GlcNAc) moiety to the ...
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PTM-ssMP: A Web Server for Predicting Different Types of Post-translational Modification Sites Using Novel Site-specific Modification Profile
... In this section, we adopt ten-fold cross-validation to evaluate the performance of ssMP in PTM site prediction based on benchmark dataset, and we assess the prediction models trained by both local sequences and ssMP are ...
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Urea induced ROS generation causes insulin resistance in mice with chronic renal failure
... by O-linked β-N-acetyl- glucosamine (O-GlcNAc), and others showed that increased modification of insulin signaling molecules by O-GlcNAc reduces insulin signal ...increased ...
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