Oligodendrocyte differentiation
Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells
... vitro oligodendrocyte differentiation for transplantation of stem cells to treat demyelinating diseases is an important ...and oligodendrocyte differentiation from human mesenchymal stem cells ...
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Genetic evidence that Nkx2 2 and Pdgfra are major determinants of the timing of oligodendrocyte differentiation in the developing CNS
... (CNS), oligodendrocyte maturation and axonal myelination occur on a predictable schedule, but the underlying timing mechanisms are largely ...of oligodendrocyte differentiation during ...early ...
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An oligodendrocyte specific zinc finger transcription regulator cooperates with Olig2 to promote oligodendrocyte differentiation
... myelinating oligodendrocyte formation representing potential downstream targets of Olig1, we screened for genes downregulated in the optic nerves of 14-day old Olig1 null mice using differential ...
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Olig1 and Olig2 promotes oligodendrocyte differentiation of neural stem cells in adult mice injured by EAE
... on oligodendrocyte differ- entiation and these newly generated oligodendrocytes were making an effort to myelinate the axons of neurons in cell culture (Figures ...induced oligodendrocyte ...
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A high throughput drug screening assay to identify compounds that promote oligodendrocyte differentiation using acutely dissociated and purified oligodendrocyte precursor cells
... A third compound class includes perphenazine, prochlorperazine, fluphenazine, trifluorperazine, and QTP: five tricyclic antidepressant molecules with struc- tural similarity believed to act primarily as serotonin/ ...
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Ecotropic Murine Leukemia Virus Infection of Glial Progenitors Interferes with Oligodendrocyte Differentiation: Implications for Neurovirulence
... FrCasE and Fr57E infection of NPCs in culture is not cyto- toxic but can reduce expression of certain glial differentiation markers in NPHs. We have previously shown that the condition- ally immortalized neural ...
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Methylthioadenosine promotes remyelination by inducing oligodendrocyte differentiation
... Methods: The effect of methylthioadenosine in promoting remyelination was tested in mouse cerebellum organotypic cultures that were exposed to lipopolysaccharide to induce neuroinflammat[r] ...
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Human oligodendrocytes from embryonic stem cells: conserved SHH signaling networks and divergent FGF effects
... and oligodendrocyte differentiation of hESCs hESCs (line H9, passage 21 to 40; H1, passage 25 to 35) were maintained as described (Thomson et ...progenitors, differentiation cultures at day 10 were ...
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The relationship between proliferation and differentiation during oligodendrocyte development
... cortical oligodendrocyte cells, rather than precursors derived from the optic nerve, that were cultured in conditioned medium for more than a week prior to ...timely differentiation of oligodendrocytes in ...
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Characterization of a murine mixed neuron-glia model and cellular responses to regulatory T cell-derived factors
... enzyme P and 1900 μl buffer X. The enzyme mix was in- cubated with the dissociated tissue for 15 min at 37 °C on a rotator. After addition of enzyme mix 2, consisting of 10 μl enzyme A and 20 μl of buffer Y, tissues were ...
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Development of a high throughput drug screening assay to identify compounds that protect oligodendrocyte viability and differentiation under inflammatory conditions
... accelerate differentiation of immature oligodendrocyte precursor cells (OPCs) into mature, myelin-producing oligodendrocytes (OLs) in the hope that this will accelerate remyelination of axons follow- ing ...
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The Wnt receptor Ryk controls specification of GABAergic neurons versus oligodendrocytes during telencephalon development
... inhibit oligodendrocyte formation and lead to the opposite phenotypes of neuron and oligodendrocyte differentiation in knockout ...under differentiation conditions for 6 ...same ...
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Transdifferentiation of human adipose-derived mesenchymal stem cells into oligodendrocyte progenitor cells
... RT-PCR results: After oligodendrocyte differentiation, total RNA extracted from differentiated cells in order to RT-PCR assay. To this end, GAPDH was used as a control marker. Our results revealed that the ...
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Promotive Effects of Yokukansan, a Traditional Japanese Medicine, on Proliferation and Differentiation of Cultured Mouse Cortical Oligodendrocytes
... the oligodendrocyte is characterized by rough endoplasmic reticulum, but its most prominent characteristic is enclosure of the axon by concentric layers of its own surface ...the oligodendrocyte cytopwlasm ...
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How to make an oligodendrocyte
... and oligodendrocyte differentiation led to the development of methods for instructing pluripotent cells to an oligodendroglial fate, first using mouse embryonic stem cells (ESCs), and, later, human (h) ESCs ...
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Transplantation of human oligodendrocyte progenitor cells in an animal model of diffuse traumatic axonal injury: survival and differentiation
... labeling. Differentiation was stud- ied with dual-label immunofluorescence combining HNu or SC121 with other oligodendrocyte markers - that is, the progenitor and early marker PDGFRα, the early markers O4 ...
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Oligodendroglia: metabolic supporters of neurons
... in oligodendrocyte metabolic ...lacking oligodendrocyte Cx47 show very mild changes in the myelin (despite 80% loss of cell coupling and total loss of astrocyte-to-oligodendrocyte coupling; ...double ...
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Collagen microsphere serving as a cell carrier supports oligodendrocyte progenitor cell growth and differentiation for neurite myelination in vitro
... For the cell transplantation and therapy, stem cells de- livered by the microspheres need to maintain their phenotype and their differentiation ability. We observed that OPCs showed the A2B5 marker in OPCs medium ...
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Netrin 1 and Dcc regulate oligodendrocyte process branching and membrane extension via Fyn and RhoA
... Fig. 8. SFK activity is required for netrin-1-induced changes in oligodendrocyte morphology. (A) Oligodendrocytes were obtained from mice lacking Fyn (Fyn KO) and treated with netrin 1 (100 ng/ml, 24 hours). Cells ...
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The Role of Wnt Signaling in Oligodendrocyte Development
... induce differentiation (Raff et ...the differentiation process, including Olig1/2 (Zhou et ...Upon differentiation, cells extend processes, contact neurons, and express myelin proteins, such as ...
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