oligonucleotide used for screening the cDNA library
Screening for plant transporter function by expressing a normalized Arabidopsis full-length cDNA library in Xenopus oocytes
... the library have no known ...the library and their corresponding predicted functions is included as supplementary information [see Additional file ...1]. Screening of transporter cDNA ...
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Identification of stroke associated antigens via screening of recombinant proteins from the human expression cDNA library (SEREX)
... Toshio Machida 1 , Motoo Kubota 2 , Eiichi Kobayashi 3 , Yasuo Iwadate 3 , Naokatsu Saeki 3 , Akira Yamaura 4 , Fumio Nomura 5 , Masaki Takiguchi 6 and Takaki Hiwasa 6* Abstract Background: Because circulating antibodies ...
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Screening of an endothelial cDNA library identifies the C terminal region of Nedd5 as a novel autoantigen in systemic lupus erythematosus with psychiatric manifestations
... By screening a cDNA library from human umbilical artery endothelial cells with serum from an SLE patient with psychosis, we identified one positive strongly reactive clone encoding the C-terminal ...
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Liu, Zhijie (2010): Identification of Novel Genes for the Development of a Rapid Diagnostic Test for Theileria uilenbergi Infection by Screening of a Merozoite cDNA Library. Dissertation, LMU München: Tierärztliche Fakultät
... In einem ersten Verfahren führten PCR Amplifikationen der Genfragmente und anschließende Sequenzierung zu der Entdeckung von 12 einzelnen Klonen. Klone 2, 9 und 26 wiesen einen hohen Grad an Identität auf, besonders am ...
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Screening lambda cdna or genomic libraries
... for cDNA library, ~10 hrs for a genomic ...the library to be plated for screening at a density such that the plaques can grow to about 1 mm in diameter before they begin to just barely touch ...
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A Lentiviral cDNA Library Employing Lambda Recombination Used To Clone an Inhibitor of Human Immunodeficiency Virus Type 1-Induced Cell Death
... system used to isolate anti-HIV genes from the library used in this ...viral cDNA library at an MOI of approximately ...of cDNA-transduced cells was estimated to be around 8 ⫻ 10 ...
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Construction and analysis of cotton (Gossypium arboreumL ) drought related cDNA library
... subtracted cDNA library is obtained, it is impor- tant to confirm that individual clones indeed represent differentially expressed ...tial screening of the subtracted library helps to ...
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Yeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
... Arabidopsis cDNA library insertion, but only 35 colonies out of that were able to show blue staining in X-gal ...of cDNA expression it might be concluded that a few proteins have a potential for true ...
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Software and methods for oligonucleotide and cDNA array data analysis
... first estimate the false-positive rate for this type of compari- son between brain regions. The best way to realistically approximate the false-positive rate is to perform and analyze independent experimental replicates ...
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Identification of immune protective genes of Eimeria maxima through cDNA expression library screening
... The effective antigens also could be masked by the dilu- tion effect during cDELI. For example, if one sub-library contains 100 plasmids during cDELI, each individual plasmid will be delivered in 1/100th of the ...
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Development of a new wheat microarray from a durum wheat totipotent cDNA library used for a powdery mildew resistance study
... Totipotent cDNA libraries representative of all the potentially expressed sequences in a genome would be of great benefit to gene expression ...a library for durum wheat (Triticum turgidum ...totipotent ...
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Increasing the library size in cDNA display by optimizing purification procedures
... First, we determined the binding capacity of streptavidin- coated magnetic beads (SA-beads) (Dynabeads MyOne streptavidin C1 streptavidin magnetic beads, Invitrogen, Carlsbad, CA, USA) sufficient to collect most of the ...
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Effective Procedure for Development of EST SSR Markers Using cDNA Library
... the cDNA library from rice ...were used to synthesize a cDNA ...the cDNA library, the 17 EST-SSR primer sets were ...from cDNA, it is possible to identify the white-backed ...
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Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI 60 cancer cells
... of cDNA clones and oligonucleotide sequences representing 1,493 UniGene clusters with r > ...all cDNA-oligo matched pairs can be found at our website [22], along with the full databases, sorted by ...
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Screening for candidate genes related to breast cancer with cDNA microarray analysis
... was used as an endogenous control with the following primers: 5 0 -CATGTACGTTGCTATCCAGGC-3 0 (for- ward primer) and 5 0 -CTCCTTAATGTCACGCAC GAT-3 0 (reverse ...
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Characterization of an Atlantic cod (Gadus morhua) embryonic stem cell cDNA library
... μl cDNA from each RT reaction for all genes was trans- ferred to a new 96-well reaction plate and the real-time PCR run in 20 μl reactions on the LightCycler ® 480 Real- Time PCR System (Roche Applied Sciences, ...
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Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger
... ESTs cDNA libraries are widely used to identify genes and splice variants, and as a physical resource for full-length clones ...SMART library provided a useful resource for the functional genomic ...
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A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide
... RNA oligonucleotide, the conjugated QDs-conjugated RNA oligonucleotide was facili- tated by spotting on an immobilized SARS-CoV N protein ...compound used as inhibitor was spotted on the conjugated ...
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An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta
... the cDNA library we noticed the presence of sev- eral viral ...ant cDNA derived from eggs, larvae and ...pupal cDNA as a ...the cDNA categories tested, additional tests would be needed ...
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An annotated cDNA library and microarray for large scale gene expression studies in the ant Solenopsis invicta
... Further characterization of viruses in fire ants may be useful for two main reasons. First, as fire ants are an invasive pest species that causes considerable economic damage in the southern USA and other locations, ...
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