Oligonucleotides, Antisense
Antisense oligonucleotides
... The genetic revolution led to the identification of many neu- rologic disease genes. The initial hope that finding the mutated protein and placing it into a known cellular pathway would lead to rapid development of ...
10
A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey
... simple antisense mechanism dependent on hybridization between the injected RNA and endogenous messenger RNA transcripts, because the later steps of the RNA interference were carried out by antisense RNA ...
23
Respirable antisense oligonucleotides: a new drug class for respiratory disease
... degrade oligonucleotides quite readily, has been success- fully addressed by a number of chemical modifications that inhibit susceptibility to cellular nucleases ...chimeric oligonucleotides that ...
5
Effect of Terminal Groups of Dendrimers in the Complexation with Antisense Oligonucleotides and Cell Uptake
... of antisense oligonucleotides (asODN) ...of antisense oligonucleotides as anticancer drugs could be a relatively easy task as they are the most dir- ect therapeutic method for mRNA blockade ...
13
Brain pharmacology of intrathecal antisense oligonucleotides revealed through multimodal imaging
... Intrathecal (IT) delivery and pharmacology of antisense oligonucleotides (ASOs) for the CNS have been successfully developed to treat spinal muscular atrophy. However, ASO pharmacokinetic (PK) and ...
20
Antisense oligonucleotides extend survival and reverse decrement in muscle response in ALS models
... Dosing of mice. Intracerebroventricular (i.c.v.) infusions were performed as previously described (45). Surgeries were conducted according to Biogen Guidance for Aseptic Rodent Surgery. Animals were anesthetized by ...
11
Reduction of integrin alpha 4 activity through splice modulating antisense oligonucleotides
... of antisense oligonucleotides as therapeutics, there is an increasing interest in expanding the application of these compounds to many other ...modulating antisense oligonucleotides to treat ...
12
Systematic approach to developing splice modulating antisense oligonucleotides
... Antisense oligonucleotides comprising 2 0 -O-methyl modified bases on a phosphorothioate backbone (2-OMe PS) were synthesised in-house on an Expedite 8909 Nucleic Acid synthesiser (Applied Biosystems, ...
12
Role of the intercellular adhesion molecule 1(ICAM 1) in endotoxin induced pneumonia evaluated using ICAM 1 antisense oligonucleotides, anti ICAM 1 monoclonal antibodies, and ICAM 1 mutant mice
... and antisense oligonucleotides are inducing more changes in endothelial cells than simply inhibiting the function or the expression of the targeted adhesion molecule, although their effects are likely to be ...
9
Efficient inhibition of HIV 1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites
... of oligonucleotides for in vivo applications. In this report the antisense effect of DNA and DNAzyme was compared to oligos that are modified with locked nucleic acid (LNA) ...the antisense oligo, it ...
13
Therapeutic potential of antisense oligonucleotides as modulators of alternative splicing
... to antisense activity of a given ...H–inactive antisense compounds with novel or estab- lished chemistry can be compared in both the cellular and the animal model, on a standard platform with a standardized ...
7
The experimental use of antisense oligonucleotides: a guide for the perplexed
... claim antisense efficacy is not acceptable. Because antisense oligonucleotides, particularly those with phospho- rothioate backbones, are sequence-nonspecifically active, it is logically circular to ...
5
Inhibition of dengue virus by novel, modified antisense oligonucleotides.
... of antisense oligonucleotides. Two antisense oligonucleotides (5 9 -AS [nucleotides 99 to 113] and 3 9 b-AS [nucleotides 10684 to 10698]) were synthesized as unmodified POs, PSs, and PrPSs ...
9
New treatments for myasthenia: a focus on antisense oligonucleotides
... Antisense oligonucleotides are designed to selectively hybridize with AChE m-RNA: this activates RNAses with subsequent degradation of the mRNA-antisense complex and aborted synthesis of the encoded ...
5
Interleukin 6 antisense oligonucleotides inhibit the growth of human myeloma cell lines
... IL-6 antisense (and not sense) oligodeoxynucleotides; in the presence of 20 microM IL-6 antisense, an 80 and 95% inhibition of the proliferation of U 266 and RPMI 8226 cells was observed, ...
5
Circular Antisense Oligonucleotides Inhibit Growth of Chronic Myeloid Leukemia Cells
... antisense circle reduced the amount of bcr-abl protein in treated cells, we used immunoblot- ting. BV173 cells were cultured with antisense or nonsense circle for 1, 2, or 3 days[r] ...
8
Antisense oligonucleotides extend survival of prion-infected mice
... Active ASOs 1 and 2 delayed the onset of prion disease clinical signs by 82% and 99%, respec- tively, compared with saline (median 250 and 272 days after infection [dpi] , respectively, [r] ...
14
Functionalized halloysite nanotube based carrier for intracellular delivery of antisense oligonucleotides
... disorders. Antisense oligodeoxynu- cleotides [ASODNs] are single-strand DNA molecules complementary to regions of a target gene that specifically inhibit gene expression by hybridizing the gene ’ s mRNA ...
7
Components of neuronal calcium channels and their interaction with GTP-binding proteins
... currents. Antisense oligonucleotides have more recently provided a novel approach to investigate which subtypes o f G protein have the principal role in coupling receptor activation to inhibition o f ...
245
Development and prevention of neural tube defects in the mouse embryo
... boundary and such genes are thought to play a role in defining this junction.. 1987; McMahon et a l 1992) and exposure of embryos to antisense oligonucleotides for either gene durin[r] ...
318