open-reading frame 1
Open Reading Frame 1 of the Norwalk-Like Virus Camberwell: Completion of Sequence and Expression in Mammalian Cells
... labeling procedure was used in all subsequent experiments with other constructs. The cells were washed with phosphate- buffered saline and cell lysates were immunoprecipitated as described previously (26) with one of ...
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Mapping of the gene coding for Epstein-Barr virus-determined nuclear antigen EBNA3 and its transient overexpression in a human cell line by using an adenovirus expression vector.
... It has been shown that the open reading frame BERFM BamHI fragment E rightward open reading frame 1, which lies within the BamHI E restriction fragment of the viral genome, codes at leas[r] ...
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In Vitro and In Vivo Characterization of a Murine Cytomegalovirus with a Mutation at Open Reading Frame m166
... m166 open reading ...multiple open reading ...specific open reading frame (1, ...m166 open reading frame results in a significant reduction of ...
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Antigenic and protein sequence homology between VP13/14, a herpes simplex virus type 1 tegument protein, and gp10, a glycoprotein of equine herpesvirus 1 and 4.
... Sequence analysis of a Agtll insert of equine herpesvirus 1 gplO identified an open reading frame in equine herpesvirus 4 with which it showed strong homology; this open reading frame al[r] ...
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A general method for nested RT PCR amplification and sequencing the complete HCV genotype 1 open reading frame
... Results: RT and nested PCR conditions were optimized independently and systematically for amplifying the complete open reading frame (ORF) from HCV genotype 1a and 1b using several overlapping ...
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The promoter, transcriptional unit, and coding sequence of herpes simplex virus 1 family 35 proteins are contained within and in frame with the UL26 open reading frame.
... In studies.reported here, a series of plasmid constructs containing deletions or insertions of an a4 promoter or of a sequence encoding a cytomegalovirus epitope reacting with a mouse mo[r] ...
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Identification and characterization of the herpes simplex virus type 1 virion protein encoded by the UL35 open reading frame.
... The 12-kDa protein recognized by the TrpE-UL35 antiserum was associated with purified HSV-1 virions and type A and B capsids, suggesting that the UL35 ORF may encode the 12-kDa capsid pr[r] ...
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The regulation of synthesis and properties of the protein product of open reading frame P of the herpes simplex virus 1 genome.
... ORF P is expressed in the absence of prior viral protein synthesis. To determine whether prior viral protein synthesis is necessary for the expression of ORF P, a cycloheximide rever- sal experiment was done. ...
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Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae
... file 1: Figure S6), has a temperature dependence such that its rate increases with growth ...file 1: Figure S6), might have different rate- limiting steps for initiation depending on a variety of factors ...
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Translational maintenance of frame: mutants of Saccharomyces cerevisiae with altered -1 ribosomal frameshifting efficiencies.
... A special site on the (+) strand of the L-A dsRNA virus induces about 2% of ribosomes translating the gag open reading frame to execute a - 1 frameshift and thus produce [r] ...
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Human Cytomegalovirus UL131 Open Reading Frame Is Required for Epithelial Cell Tropism
... Assays for virus infection and replication. To monitor the efficiency of infec- tion, epithelial cells, endothelial cells, or fibroblasts were transferred into 12-well plates and incubated overnight to produce ...
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Norwalk Virus Open Reading Frame 3 Encodes a Minor Structural Protein
... different reading frames and the only known promoter driving the transcription of these constructs lies upstream of the ORF 2 coding ...ribosomal frame- shifting, internal initiation, or ...
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Reverse genetic characterization of the natural genomic deletion in SARS Coronavirus strain Frankfurt 1 open reading frame 7b reveals an attenuating function of the 7b protein in vitro and in vivo
... During the outbreak of SARS in 2002/3, a prototype virus was isolated from a patient in Frankfurt/ Germany (strain Frankfurt-1). As opposed to all other SARS-Coronavirus strains, Frankfurt-1 has a ...
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Attenuation of Marek's Disease Virus by Deletion of Open Reading Frame RLORF4 but Not RLORF5a
... It is interesting that when the levels of in vivo replication and oncogenesis for RB-1B/p17 and rRB-1B were compared, the latter replicated at significantly lower levels and MD occurred with kinetics that were delayed by ...
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Bovine herpesvirus 1 U(s) open reading frame 4 encodes a glycoproteoglycan.
... Infection of cells in the presence of castanospermine con- firmed the conclusion that gG is N-glycosylated. Since castano- spermine inhibits formation of complex N-linked oligosaccha- rides (for a review, see reference ...
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Demonstration of biological activity and nucleotide sequence of an in vitro synthesized clone of the Moloney murine sarcoma virus mos gene.
... In the three plus strand reading frames, which have the same sense as the Mo-MSV viral RNA genome, there is clearly only a single large open reading frame in frame 1, whose predicted ami[r] ...
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E5 open reading frame of bovine papillomavirus type 1 encodes a transforming gene.
... In C127 cells, pHLB1 normally induced only about one-half as many foci as the full-length BPV clone, Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest MATERIALS AND METHO[r] ...
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Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
... fusion protein was performed as described by the manufacturer (Qiagen), with slight modification. BV-32-infected SF9 cells were pelleted and resuspended in binding buffer (50 mM sodium phosphate buffer [pH 8.0], 300 mM ...
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Telomeric Silencing of an Open Reading Frame in Saccharomyces cerevisiae
... noted deletions and sir3. WT, wild-type strain D139-11B; DI, chromosome I deletion strain DK420-1D; DI DVIII, chromo- somes I and VIII double-deletion strain DK421-2D; sir3DVIII, sir3Ttrp1 chromosome VIII deletion strain ...
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Mutational analysis of open reading frame E4 of bovine papillomavirus type 1.
... Transformed cell lines were established from a plate containing >50 foci induced by the indicated viral DNAs.. Cells were then grown in 0.3% agarose in Dulbecco modified Eagle medium-10%[r] ...
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