P. furiosus
Divergence of the Hyperthermophilic Archaea Pyrococcus furiosus and P. horikoshii Inferred From Complete Genomic Sequences
... is P. furiosus motile if it is lacking the genetic capability for chemotaxis? It is possible that an- other system of taxis exists, unrelated to the bacterial type found so far in the Euryarcheota and ...
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Superoxide Reductase from the Hyperthermophilic Archaeon Pyrococcus furiosus: its Function, Regulation, and Biotechnological Applications
... aerobes. P. furiosus employs a novel enzyme system centered on the enzyme superoxide reductase (SOR), which reduces superoxide molecules to hydrogen peroxide without producing ...Surprisingly, P. ...
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Modeling and structural analysis of PA clan serine proteases
... from P. furiosus had 7 β-sheets with His286 situated in a turn, Asp320 in a coil and Ser389 in a turn (Figure ...the P. furiosus protease had a slightly higher proportion (> SD of the mean) ...
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Development of the Extreme Thermophiles Pyrococcus furiosus and Sulfolobus acidocaldarius as Metabolic Engineering Platforms.
... The pace of metabolic engineering of extreme thermophiles is largely influenced by practical factors in generating mutant strains. The ease of use of a genetic system, the time required to generate a mutant, and the ...
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Biochemical and biophysical characterization of compartmentalizing proteases from the hyperthermophilic microorganism Pyrococcus furiosus
... The purified forms of the recombinant β1 and β2 subunits appeared in as many as 3 individual bands per subunit on a 10% native PAGE, suggesting that they were able to form assembled structures. However, the β subunits ...
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Discovery, Functional Genomics and Biochemical Characterization of Alpha-specific Glycosyl Hydrolases from Hyperthermophilic Microorganisms
... PF1108 is annotated as a ‘putative α-dextrin-endo-1,6-α-glucosidase’ and its strongest domain homology is to the α/β superfamily hydrolase. The α/β superfamily hydrolase is a large grouping of enzyme with greatly varied ...
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Functional Characterization of MoeA and MoeB Tungsten Cofactor Synthesis Proteins from the Hyperthermophilic Archaeon Pyrococcus furiosus
... His-tagged P. furiosus MoeA, MoeA2, and MoeB ...recombinant P. furiosus proteins, cells from each expression were lysed and then heat-treated to denature the majority of the ...
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Functional Evaluation of the Mechanisms of Reactive Oxygen Detoxification in the Hyperthermophilic Archaeon Pyrococcus Furiosus Using Complementation Studies
... As an alternate method, the cloning vector, pPCR-Script was employed to clone these two genes as a single sequence. Because the restriction enzyme sites of the Rd-SOR PCR product are located at the ends of the DNA ...
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Transcriptomic and Physiological Analysis of a Recombinant Pyrococcus furiosus Strain Metabolically Engineered to Produce 3-Hydroxypropionate from CO2 and Maltose.
... mM; initial testing of P. furiosus at these 3-HP levels indicates a comparable effect (communication with Gerrit J. Schut, University of Georgia). Also, addition of 3-HP to the growth medium impairs the ...
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Characterization of the Dinuclear Metal Center of Pyrococcus furiosus prolidase and Production of its Mutants with Increased Activity at Low Temperatures
... Using the positive selection strategy we designed, we successfully screened two cold-adapted P. furiosus prolidase variants, G39E- and E236V-prolidases, which had significant increased activity at low ...
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Functional Analysis of Tungsten Metabolism in Pyrococcus furiosus
... reduces P. furiosus MoeA2 function, and that there are inherent differences in substrate donors between the ...and P. furiosus cofactor synthesis ...for P. furiosus proteins ...
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Functional Genomics Analysis of Biohydrogen Production by Hyperthermophilic Microorganisms
... Thus, it appears that growth on maltose is characterized by an imbalance between substrate uptake and subsequent disposition of reducing equivalents. The elevated expression of several stress response proteins supports ...
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Heterogeneity of proteinases from the hyperthermophilic archaeobacterium, Pyrococcus furiosus.
... of P. furiosus ctW extracts and culture supernatants using gelatin SDS PAGE, suggests that dissociation of the proteinases under denaturing conditions can be ...in P. furiosus may represent a ...
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<p>Refractory Sarcoidosis: A Review</p>
... Recently, Sharp et al reported four patients with multi- drug-resistant sarcoidosis treated with TCZ (one case of pulmonary sarcoidosis, one of cutaneous sarcoidosis, one of sarcoidosis [r] ...
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Exploring the spin states of 90Zr populated by (p,p’), (p,d), and (p,t) reactions
... A schematic view of the experimental set up is shown in Fig. 1. The energy spectra and angular distribution of the light ions and prompt γ -rays were measured with the STARLiTeR detector system [11]. The STARLiTeR ...
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|P| ) time where |P| is
... Then, they are defined by the form of character :<the occurrence position of character in pattern: the indicated status of the last character of pattern: the number of pattern in P>; e.g., a:<1:0:1>, ...
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Resonance production in p+p, p+A and A+A collisions measured with HADES
... from p+Nb collisions with momenta larger and smaller 0.8 GeV/c to the p+p data in the same kinematic regions scaled to the number of participants and the total reaction cross ...with P ee < ...
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<p>Organ Donation In Bahrain</p>
... There were three types of organ transplants in Saudi Arabia: kidney transplants, with 294 surgeries, 208 from living donors and 86 from deceased donors; liver transplants, with 70 surger[r] ...
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<p>Genetics of COPA syndrome</p>
... The etiopathogenesis of COPA syndrome is unknown, but it is hypothesized that disturbances in protein trafficking pathway can lead to endoplasmic reticulum (ER) stress that results in[r] ...
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<p>Accountability in patient adherence</p>
... Qurient, P fi zer ...Mylan, P fi zer Inc, Qurient, Sano fi , Sienna, Sun Pharma, Suncare Research, Valeant, and Xenoport, is the founder, chief technology of fi cer and holds stock in Causa Research, holds ...
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