PCR Analysis for bacterial samples
Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR
... real-time PCR using group-specific ...real-time PCR detection system (Agilent, ...Green PCR Mix (TaKaRa, Japan), 1 μl of each primer (20 μM) (Table 1), 1 μl of sam- ple DNA and ...real-time ...
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Quantitative PCR Assessments of Bacterial Species in Women with and without Bacterial Vaginosis
... microscopic analysis of vaginal swab ...vaginal samples from six women with normal vaginal floras who had Nugent scores of 0 and exhibited none of the Amsel’s criteria and from nine women with BV who had ...
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Improvement of Detection of Bacterial Pathogens in Normally Sterile Body Sites with a Focus on Orthopedic Samples by Use of a Commercial 16S rRNA Broad Range PCR and Sequence Analysis
... time PCR assays, on the other hand, facilitate the rapid identification of pathogens and are a promising approach for rou- tine ...diagnosing bacterial infections ...
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Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
... After a gentle supramucosal cleaning, submucosal plaque samples and peri-implant sulcus fluid were collected at the deepest aspect of each implant site by means of sterile paper points (i.e., each was left in ...
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Identification of Bacterial Pathogens in Ascitic Fluids from Patients with Suspected Spontaneous Bacterial Peritonitis by Use of Broad Range PCR (16S PCR) Coupled with High Resolution Melt Analysis
... patient samples collected under this protocol were sent for clinical microbiological diagnostics to identify bacterial pathogens in the peritoneal fluid from these patients under standard of care ...
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Analysis of Chlamydia pneumoniae Growth in Cells by Reverse Transcription-PCR Targeted to Bacterial Gene Transcripts
... the PCR for human -actin was also ...Each PCR condition was optimized before being applied to ...The PCR cycle consisted of a 5-min denaturation at 95°C followed by 25 to 40 cycles each of 45 s of ...
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Real-time PCR analysis of enteric pathogens from fecal samples of irritable bowel syndrome subjects
... on bacterial culturing techniques possibly lacking the sufficient sensitivity for detecting low levels of pathogens from fecal ...pathogenic bacterial species or groups are associated with IBS in comparison ...
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Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants
... In order to define the detection limit of the OralArray, LDR-UA experiments were carried out with decreas- ing amounts of 16 S rRNA amplicons, in the range from 8 to 1 ng (corresponding to 5.6–0.7 fmol, respectively). ...
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Targeted PCR for Detection of Vaginal Bacteria Associated with Bacterial Vaginosis
... bacterium-targeted PCR assays to vaginal-fluid samples serves several ...the bacterial compositions of the human vagina in subjects with and without BV and validate our earlier findings from a ...
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Bacterial and fungal microflora in surgically removed lung cancer samples
... chronic bacterial or fungal infections ...as PCR-RFLPs, Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and microcolony methods allow examination and ...
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Quantification of Zoonotic Bacterial Pathogens within Commercial Poultry Processing Water Samples Using Droplet Digital PCR
... 4. Conclusion These findings represent the first report of the use of third generation PCR technology to detect zoonotic bac- terial pathogen signatures in environmental samples along the poultry production ...
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Analysis of the Effect of DNA Purification on Detection of Human Papillomavirus in Oral Rinse Samples by PCR
... ber-standardized samples that were comparable to the 91% overall agreement in HPV infection status observed with cer- vical samples collected by different methods on the same day (9) as well as the same ...
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Cytomegalovirus PCR Analysis of Serum and Ocular Swab Samples in Patients with Inflammatory Eye Disease
... 150 samples were divided into two ...by PCR method using Research thermal ...of samples, extraction is carried out with the Qiagen ...Blood samples delivered to the laboratory on the same day ...
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Detection of vancomycin resistant enterococci in fecal samples by PCR
... perirectal samples with Enterococcosel-V agar after enrichment in supplemented CNA broth at 35°C followed by biochemical species identifi- cation and broth microdilution susceptibility testing was the most ...
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PCR for detection of rubella virus RNA in clinical samples
... Rubella virus isolation. Isolation of rubella virus from pharyngeal swabs, PBMCs, and POCs was performed as described by Best and O’Shea (4). POCs (fresh or material that had been stored at 2708C) were washed thoroughly ...
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Typing and subtyping of influenza viruses in clinical samples by PCR
... Direct immunofluorescent and rapid commercial enzyme immunoassay methods of influenza virus antigen detection may have shorter times to a positive result than RT-PCR, but they may have decreased sensitivities and ...
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Factors Contributing to Variability of Quantitative Viral PCR Results in Proficiency Testing Samples: a Multivariate Analysis
... While viral load testing has gained widespread acceptance, a primary limitation remains the variability of results, particularly between different laboratories. While some work has demonstrated the importance of ...
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Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
... for analysis using molecular ...water samples, filtrated and concentrated with the use of special laboratory ...in PCR mixes at ten different ...stool samples spiked with ...Nested PCR ...
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Enrichment of bacteria samples by centrifugation improves the diagnosis of orthopaedics related infections via real time PCR amplification of the bacterial methicillin resistance gene
... of bacterial DNA that is still contained within dying or recently dead bac- ...of PCR inhibitors and impurities in the extracted DNA and reduce the sensitivity of the ...
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High Resolution Melt PCR Analysis for Genotyping of Ureaplasma parvum Isolates Directly from Clinical Samples
... (HRM) PCR assay for the differ- entiation of the four serovars of ...for PCR amplification and HRM analysis to generate reproducible and distinct melt profiles that distinguish clonal representatives ...
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