PCR and sequencing
Serotype Identification of Group B Streptococci by PCR and Sequencing
... to PCR and sequencing capabilities, and, importantly, does not require panels of sero- type-specific antisera, which are increasingly difficult to main- ...and sequencing of a rela- tively limited ...
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Comprehensive Detection and Serotyping of Human Adenoviruses by PCR and Sequencing
... simple PCR assay that uses a single primer pair for the rapid, sensitive detection of all known human adenoviruses and permits accurate typing by sequencing of the ...
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Sequenase Version 2.0 PCR Product Sequencing Kit
... PCR and sequencing reactions are typically run in microcentrifuge tubes (0.5ml) or 96-well plates. These should be kept capped to minimize evaporation of the small volumes employed. Additions should be made ...
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Sequetyping: Serotyping Streptococcus pneumoniae by a Single PCR Sequencing Strategy
... This new sequetyping method has multiple advantages over other previously reported serotyping methods. It requires only a single PCR amplification. The reaction setup is straightforward, robust, and economical, ...
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Babesia canis canis in dogs from Hungary: detection by PCR and sequencing
... using PCR and sequence analysis was attempted to clarify the subspecies (genotype) and to obtain information on the occurrence of ...piroplasm-specific PCR amplifying the partial 18S rRNA gene yielded an ...
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ECKER LAB PROTOCOL. Nested PCR and Sequencing of T-DNA Junctions in Arabidopsis
... Nested PCR and Sequencing of T-DNA Junctions in Arabidopsis Plant Growth Conditions The germination and DNA extraction of individual lines are done in 96-deep-well plates (Doc Frugal #33-196 or Beckman ...
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DNA Sequencing and PCR Markets
... DNA Sequencing and PCR Markets Description: DNA sequencing and polymerase chain reaction (PCR) are two major technology platforms of value in life science research, drug discovery and clinical ...
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Polyadenylated Sequencing Primers Enable Complete Readability of PCR Amplicons Analyzed by Dideoxynucleotide Sequencing
... using sequencing primers with 40–80 bp neutral polynucleotide tails ...mixed sequencing data on the initial part of analyzed ...of PCR amplicons using sequencing primers modified by ...
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Automated degenerate PCR primer design for high throughput sequencing improves efficiency of viral sequencing
... degenerate PCR primer design system that plays a key role in the ...population. PCR primer pairs are then selected computationally to produce a minimal amplicon set capable of tiling across the full length ...
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Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
... enrichment PCR step as the primary source of base-composition bias in frag- ment libraries and developed an optimized PCR proto- col that produces libraries that are far less skewed than standard ...
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Hibbett lab protocols for DNA isolation, PCR, and DNA sequencing.
... cycle sequencing. A ready sequencing reaction represents a mixture of terminated fragments, which are labelled with one of the four dye molecules at a ...
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Microsatellite instability in prostate cancer by PCR or next-generation sequencing
... Although our primary objective was to compare the per- formance of expanded-marker NGS assays to MSI-PCR, we also evaluated the performance of IHC when this in- formation was available (Additional file 2: Table ...
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Comparative Study of Direct Bisulfite Sequencing PCR and Methylation Specific PCR to Detect Methylation Pattern of DNA
... bisulfite sequencing, MSP, combined bisulfite wrong ...etc. sequencing is a more confident method mainly known as Bisulfite direct sequencing is the most a quantitative method representing precise ...
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Differentiation of Mycobacterial Species by hsp65 Duplex PCR Followed by Duplex PCR Based Restriction Analysis and Direct Sequencing
... multiplex PCR methods have intrinsic problems due to the use of multiple target genes and lack of conservation in target ...duplex PCR targeting the RNA poly- merase gene could overcome problems caused by ...
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Microbiome Profiling by Illumina Sequencing of Combinatorial Sequence-Tagged PCR Products
... to PCR primers that amplify the rRNA V6 region. Amplified PCR products are sequenced using an Illumina paired-end protocol to generate millions of overlapping ...that PCR-induced errors composed the ...
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Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation
... PEP P-element 3' end Pry4 Pry1 55° PEP P-element 3' end Pry2 Pry1 60° The Pry2/Pry1 combination has a higher annealing temperature than the Pry4/Pry1 and Pry4/Plw3-1 combinations, but the resulting PCR products do ...
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Multiplex PCR and next generation sequencing for the non invasive detection of bladder cancer
... NGS-amplicon sequencing on urine DNA When tested individually, all 16 primer pairs generated the correct size ...multiplex PCR tested over a range of template concentrations (designed to mimic the variable ...
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Comparison of Sequencing (Barcode Region) and Sequence Tagged Site PCR for Blastocystis Subtyping
... Site PCR for Blastocystis Subtyping Christen Rune Stensvold Unit of Mycology and Parasitology, Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark Blastocystis is the most ...
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Molecular diagnosis of bacterial endocarditis by broad range PCR amplification and direct sequencing
... broad-range PCR amplification followed by direct sequencing is a relatively easy and reliable method that gives accurate results when applied to surgically removed heart valves of patients with IE and may ...
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Direct Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis by Use of pncA PCR Sequencing
... pncA sequencing performed on subsequent isolates revealed no pncA mutation, and the specimens were shown to be phenotypically susceptible to PZA, with positive PZase ...pncA PCR step, which might also be ...
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