PCR assay
Multiplex Real Time PCR Assay for Detection of Influenza and Human Respiratory Syncytial Viruses
... multiplex PCR respiratory assay for de- tection of ...real-time PCR assay and the pres- ence of HRSV antigens by use of the RSV TestPack (Table ...multiplex PCR assay only, and ...
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Clinical evaluation of a Mycobacterium tuberculosis PCR assay
... of PCR was 100%. However, since the PCR assay was also positive in a number of patients with only prior or treated Tb or asymptomatic tuberculosis infection, the specificity for a diagnosis of active ...
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Detection of Pulmonary Tuberculosis by PCR Assay
... on PCR reaction ...of PCR interfering substances or a source of cross-contamination between clinical specimens ...of PCR assay in identifying ...
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Multiplex LightCycler PCR Assay for Detection and Differentiation of Bordetella pertussis and Bordetella parapertussis in Nasopharyngeal Specimens
... multiplex PCR assay for detecting and differentiating Bordetella pertussis and Bordetella parapertussis in nasopharyngeal swabs was ...This assay (LC-PCR-IS) targets the insertion sequences ...
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Quantitative Analysis of Epstein Barr Virus Load by Using a Real Time PCR Assay
... tected PCR inhibitors and estimated the efficiency of the ex- traction methods, both of which are particularly important to quantify the EBV load accurately and ...conventional PCR assays, which have ...
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5′ Nuclease PCR Assay To DetectYersinia pestis
... conventional PCR assays reported by Hinnebusch and Schwan (7, 8), 90% of engorged fleas were positive; preparation of the fleas was simple, involving homog- enization in 50 ml of brain heart infusion broth, ...
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Detection and identification of aquatic birnaviruses by PCR assay
... RT-PCR assay to directly detect aquatic birnavirus in infected fish, several types of clin- ical specimens were collected from a population of naturally infected brook trout that were IPNV ...RT-PCR ...
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A triplex quantitative real time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
... tq-PCR assay in this study is more convenient and rapid to detect the most common HAdV serotypes 2, 3 and 7 in a single closed tube, thus it is very suitable for HAdV typing to meet clinical diagnosis ...
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Methicillin Resistant Staphylococcus aureus (MRSA) Detection: Comparison of Two Molecular Methods (IDI MRSA PCR Assay and GenoType MRSA Direct PCR Assay) with Three Selective MRSA Agars (MRSA ID, MRSASelect, and CHROMagar MRSA) for Use with Infection Control Swabs
... (IDI-MRSA PCR assay and GenoType MRSA Direct PCR assay) and three selective MRSA agars (MRSA ID, MRSASelect, and CHROMagar MRSA), using 205 (101 nasal, 52 groin, and 52 axillary samples) ...
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Evaluation and Validation of Real Time Reverse Transcription PCR Assay Using the LightCycler System for Detection and Quantitation of Norovirus
... an assay for the detection and quantitation of norovirus with the LightCycler SYBR Green- based real-time reverse transcription-PCR (real-time LC RT-PCR) and previously published primers in the ...
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Rapid Identification of Yersinia enterocolitica in Blood by the 5′ Nuclease PCR Assay
... TaqMan assay has been recently described ...the PCR is ...this assay seemed to have the potential for such ...TaqMan PCR assay is described which is rapid and shows specific and ...
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A TaqMan Probe Based Real Time PCR Assay for the Rapid Identification of the Emerging Multidrug Resistant Pathogen Candida auris on the BD Max System
... real-time PCR assay on the BD Max platform targeting ribosomal DNA (rDNA) region nucleotide sequences to quickly and accurately test for ...The assay is highly specific, reproducible, and sensitive, ...
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Nested PCR Assay for Detection of Granulocytic Ehrlichiae
... 5). PCR assays have been used often, mostly in research-oriented settings, and have the advantage of being able to yield positive results during the early acute phase of ...illness. PCR-based assays have ...
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Development of a PCR Assay for Rapid Detection of Enterococci
... Enterococcus-specific PCR assay indicates a high level of similarity of the tuf genes at the primer binding sites in the genus Abiotrophia and the genus ...that PCR primers derived from the tuf ...
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External Quality Assessment for Enterovirus 71 and Coxsackievirus A16 Detection by Reverse Transcription PCR Using Armored RNA as a Virus Surrogate
... Statistical analysis. Data collected were entered into a spreadsheet in Mi- crosoft Excel (Microsoft Corp., Bellingham, WA) and analyzed using SPSS 13.0 for Windows. The rates of correct responses for EV-, EV71-, and ...
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Direct Detection of Sabin Poliovirus Vaccine Strains in Stool Specimens of First Dose Vaccinees by a Sensitive Reverse Transcription PCR Method
... RT-PCR assay could be used to monitor the impact of the change in the vaccination schedule on virus ...nested PCR assay to assess the shedding of Sabin strains in stools from vaccinees who ...
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Current Trends in Rapid Diagnostics for Methicillin Resistant Staphylococcus aureus and Glycopeptide Resistant Enterococcus Species
... real-time PCR assay and is an FDA-approved assay for the direct detection of nasal colonization by ...This assay can be semiautomated using the SmartCycler in- strument ...This assay ...
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Mastitis pathogen identification using polymerase chain reaction in New Zealand milk samples : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Animal Science at Massey University, Palmerston North, New Zealand
... Figure 1. Internal Amplification Control curves obtained from a 93-bp fragment of lambda-DNA included in the Primer Mixes 1, 2, 3, and 4 of the PathoProof Mastitis PCR Assay. Illustrated are the IAC ...
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Direct Detection of Erythromycin Resistant Bordetella pertussis in Clinical Specimens by PCR
... allele-specific PCR method for rapid detection of erythromycin-resistant ...allele-specific PCR assay. The methods were verified with results obtained by PCR-based sequencing of 16 recent ...
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Rapid PCR detection of group a streptococcus from flocked throat swabs: A retrospective clinical study
... There are several limitations to our study. First, not all specimens collected in the original flocked swab study were available for testing with the PCR assay. However, the proportion positive for GAS by ...
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