PCR-based analysis of pooled bee samples:
Fragmentation of pooled PCR products for highly multiplexed TILLING
... of PCR products, proper quantification and pooling of sequenc- ing libraries, and producing sufficient read coverage to ensure recovery of rare mutations based on how many genomic DNA samples are ...
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Bayesian Inference for the Left Truncated Exponential Distribution Based on Pooled Type-II Censored Samples
... reliability analysis, experiments are often terminated before all units on test fail based on cost and time ...censored samples in order to increase the precision of the ...
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Effective detection of rare variants in pooled DNA samples using Cross pool tailcurve analysis
... a PCR method for target enrichment in this study, other popular enrichment methods, such as microarray Table 3 Validation analysis of variant calling from second cohort ...
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Comparison of Microscopy, Nested PCR, and Real Time PCR Assays Using High Throughput Screening of Pooled Samples for Diagnosis of Malaria in Asymptomatic Carriers from Areas of Endemicity in Myanmar
... Blood samples (n ⴝ 1,552) were collected from residents in three areas of malaria endemicity (Kayin State, Bago, and Tanintharyi regions) of ...nested PCR and real-time PCR assays showed that ...
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Evaluation of microarray preprocessing algorithms based on concordance with RT-PCR in clinical samples.
... control genes was found to be an accurate normalization factor for RT-PCR measurements [29]. Although the accuracy of preprocessing algorithms for Affyme- trix microarrays could be compared in numerous ways, we ...
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A Novel and Rapid PCR Based Method for Genotyping Human Papillomaviruses in Clinical Samples
... three samples could be genotyped. Samples 1 and 3 were found to contain HPV-16; sample 2 was found to contain ...the analysis of large numbers of clinical samples would require fast-through- ...
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Diagnosis of Bacteremia in Whole Blood Samples by Use of a Commercial Universal 16S rRNA Gene Based PCR and Sequence Analysis
... The PCR analysis can be performed within a working day and provided information on the infec- tious state of patients earlier than almost 70% of the BC in the present ...by PCR requires, however, ...
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Analysis of the Effect of DNA Purification on Detection of Human Papillomavirus in Oral Rinse Samples by PCR
... K-digested samples could be attributed to the presence of PCR inhibitors in the oral rinse samples, as previously ob- served by our group (16) and others (25, ...of PCR inhibition from sample ...
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Adjuvant chemotherapy for rectal cancer with complete pathological response (pCR) may not be necessary: a pooled analysis of 5491 patients
... all based on the National Cancer Database ...in pCR, all performed ...with pCR might undergo local resection, instead of TME, devoid of reginal lymph nodes ...
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PCR Based Detection of Toxoplasma gondii DNA in Blood and Ocular Samples for Diagnosis of Ocular Toxoplasmosis
... blood), PCR methods appear to be more effective for immunocompro- mised patients than for their immunocompetent ...now, PCR for Toxoplasma genome detection using whole blood does not offer sufficient ...
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Evaluation of PCR-Based Assay for Diagnosis of Spotted Fever Group Rickettsiosis in Human Serum Samples
... nested PCR assay was developed for the detection of spotted fever group (SFG) rickettsiae in serum ...was based on specific primers derived from the rickettsial outer membrane protein B gene (rompB) of ...
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Pharmacogenomics of platinum-based chemotherapy response in NSCLC: a genotyping study and a pooled analysis
... SNP selecting, DNA extracting and genotyping By considering the meta-analysis of pharmacogenomics of platinum-based chemotherapy in NSCLC, we searched the publications which reported the associations of ...
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Optimizing a PCR protocol for cpn60 based microbiome profiling of samples variously contaminated with host genomic DNA
... target based microbiome profiling includes universal PCR of microbiome samples across an annealing temperature gradient to maximize the diversity of sequences ...universal PCR primer cocktail ...
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Multiplex pcr based detection of leptospira canicola in environmental water samples obtained from nagapattinam area
... water samples collected from different locations had positive reactions according to multiplex PCR ...protocol, based on the protocol previously used to assess clinical samples in our ...
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Real-time PCR analysis of enteric pathogens from fecal samples of irritable bowel syndrome subjects
... real-time PCR assays Quantitative real-time PCR procedures, which utilized 12 previously designed oligonucleotide primer pairs for the detection of an extensive set of intestinal pathogens, were optimized ...
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High Resolution Melt PCR Analysis for Genotyping of Ureaplasma parvum Isolates Directly from Clinical Samples
... clinical samples is that, in cases of colonization by multiple serovars, we were unable to determine the serovar combinations present based upon melt curve analyses and instead could identify these only ...
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Cytomegalovirus PCR Analysis of Serum and Ocular Swab Samples in Patients with Inflammatory Eye Disease
... 150 samples were divided into two ...by PCR method using Research thermal ...of samples, extraction is carried out with the Qiagen ...Blood samples delivered to the laboratory on the same day ...
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A pooled analysis of mesenchymal stem cell-based therapy for liver disease
... sensitivity analysis altered this result at two time ...of pooled studies that could be used to assess an outcome at a par- ticular time point, especially longer time points, was lim- ...
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Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan Based PCR Assay
... By using the TaqMan assay, the smallest amount of C. albi- cans DNA tested and reliably quantified is 5 CFU/ml of blood with either the species-specific or the genus-specific probe. In practice, however, a relatively ...
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PCR assay based on DNA coding for 16S rRNA for detection and identification of mycobacteria in clinical samples
... cross-hybridizing PCR products were derived belonged to nonpathogenic or nonopportunistic species which do not occur in clinical ...The samples included sputum, bronchoalveolar lavage, tissue biopsy ...
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