PCR, cloning and sequencing
Detection and Identification of Mycobacterium tuberculosis in Joint Biopsy Specimens by rpoB PCR Cloning and Sequencing
... However, the differential diagnosis of M. tuberculosis arthritis and rheumatoid arthritis is problematic, especially in patients with joint biopsy samples that have nonspecific results by his- tological evaluation and ...
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Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad range PCR, cloning and sequencing
... Broad-range PCR, sequencing and cloning are essential tech- niques for the characterization of the microbial environment in ...rDNA PCR coupled to automated DNA extraction to identify ...
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Broad range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian patients with reactive and undifferentiated arthritis
... broad-range PCR amplification, cloning, and sequencing of the full-length 16S rDNA from a wide variety of bacterial species in the SF samples of all patients ...
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Genome sequencing accuracy by RCA-seq versus long PCR template cloning and sequencing in identification of human papillomavirus type 58
... deep sequencing (RCA-seq) and by long PCR template cloning and ...template cloning and sequencing in decoding HPV58 ...long PCR template cloning and sequencing. ...
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CHAPTER 6. CLONING AND SEQUENCING OF CCCVd-RELATED RT-PCR PRODUCTS
... The occurrence of these variants agrees with the RPA analysis (Chapter 4), which showed that the CCCVd-like RNAs in this palm had several mismatches when compared with CCCVd. The presence of sequences equivalent to OP ...
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Five-minute cloning of Taq polymerase-amplified PCR products for sequencing
... Transformants by PCR You may wish to use PCR to directly analyze positive transformants. You may use one of the four primers in the kit (M13 Forward (-20), M13 Reverse, T3, or T7) in combination with a ...
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Characterization of a circulating PRRSV strain by means of random PCR cloning and full genome sequencing
... sequence-independent PCR techniques for the detection and characterization of PRRSV could be useful to bypass problems associated with the heterogeneity of this ...
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Cloning and Sequencing of a Part of the Heat Shock Protein 65 Gene (hsp65) of “Tropheryma whippelii” and Its Use for Detection of “T whippelii” in Clinical Specimens by PCR
... whippelii”-specific PCR assay was further assessed by digesting amplicons with XhoI in order to confirm that the amplified products indeed showed the restric- tion pattern which, according to available hsp65 ...
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pcr 8/GW/TOPO TA Cloning Kit
... the PCR product insertion site, and fall within the attL1 and attL2 sites, respectively of pCR ™ 8/GW/TOPO ® ...in sequencing inserts cloned into pCR ™ 8/GW/TOPO ® ...in pCR ™ 8/GW/TOPO ...
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CLONING, SEQUENCING AND EXPRESSION OF THE OVINE INTERLEUKIN-6 GENE
... transcriptase PCR with heterologous primers has been used to obtain a cDNA clone encoding the structural sequences of ovine interleukin 6 from alve- olar ...
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Cloning, Sequencing and Phylogenetic Analysis of Stn Gene of Salmonella Typhimurium
... The desired product was successfully purified using QIA quick gel extraction kit and cloned in pJET 1.2 blunt cloning vector (Fermentas, USA) and transformed into chemically competent E.coli DH5 á cells. ...
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Sequenase Version 2.0 PCR Product Sequencing Kit
... DNA sequencing method in those patents solely for use with Sequenase DNA polymerase purchased from USB Corporation for research ...for cloning or producing nucleotide sequences for commercial ...
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Efficient Cloning of Alternatively Polyadenylated Transcripts via Hybridization Capture PCR
... that were supported by oligo(dT) based methods may not be authentic and seem to derive from internal-priming artifacts. Although RNA-Seq is a very attractive approach to transcriptome profiling, it is still a technology ...
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Cloning and Sequencing of a Candida albicans Catalase Gene and Effects of Disruption of This Gene
... Received 19 June 1997/Returned for modification 5 August 1997/Accepted 25 February 1998 Catalase plays a key role as an antioxidant, protecting aerobic organisms from the toxic effects of hydrogen peroxide, and in some ...
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IDENTIFICATION, ISOLATION, CLONING AND SEQUENCING APARTIALANNEXIN GENE FROM AUREOBASIDIUM PULLULANS
... for sequencing following plas mid DNA ...bp PCR product was analysed by BLASTX, the translated sequence showed a significant homology to fungal and other eukaryotic annexin genes ...
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Cloning and Sequencing the First HLA Gene
... DNA sequencing at the Laboratory of Molecular Bi- ology, Cambridge (UK), with a close associate of Fred Sanger, George Brownlee, who, strangely enough, was not using Sanger sequencing (S anger et ...direct ...
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Molecular Cloning, Sequencing, and Functional Expression of a
... An aspartate residue located in the second transmembrane domain and playing a key role in binding affinity and functional coupling of other 7-TMS receptors is present in po[r] ...
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DNA Sequencing and PCR Markets
... DNA Sequencing and PCR Markets Description: DNA sequencing and polymerase chain reaction (PCR) are two major technology platforms of value in life science research, drug discovery and clinical ...
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PCR & DNA Sequencing. PCR= Polymerase Chain Reaction. PCR applications
... of PCR reaction: • As a rule of thumb, use an annealing temperature (Ta) about 5 o C below the lowest Tm of the pair of primers to be used if a good yield of product is ...
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Easy-A High-Fidelity PCR Cloning Enzyme
... High-Fidelity PCR Cloning Enzyme N OTICE T O P URCHASER Limited Label License: The purchase of this product conveys to the purchaser only the limited, non- transferable right to use the purchased quantity ...
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