PCR Fingerprinting
Suitability of Repetitive DNA Sequence Based PCR Fingerprinting for Characterizing Epidemic Isolates of Salmonella enterica Serovar Saintpaul
... primed PCR (AP-PCR) fin- gerprinting, and, lately, repetitive-sequence-based PCR (rep- PCR) fingerprinting have been extensively used to characterize strains of various bacterial ...
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Study of Molecular Epidemiology of Candidiasis in Portugal by PCR Fingerprinting of Candida Clinical Isolates
... A preliminary characterization of the 177 yeast strains iso- lated by a rapid identification kit indicated that they all be- longed to the genus Candida, although several doubts arose regarding species identity. ...
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Molecular Analysis of a Hospital Cafeteria Associated Salmonellosis Outbreak Using Modified Repetitive Element PCR Fingerprinting
... A hospital cafeteria-associated outbreak of gastroenteritis due to Salmonella enterica serotype Infantis was retrospectively evaluated using modified repetitive element PCR (rep-PCR) fingerprinting ...
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PCR Fingerprinting of Candida albicans Associated with Chronic Hyperplastic Candidosis and Other Oral Conditions
... each PCR product was added to 4 l of gel loading buffer ...the PCR products and to perform gel normalization, molecular weight mark- ers consisting of a 100-bp DNA ladder (Life Technologies ...the ...
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Improved Repetitive-Element PCR Fingerprinting ofSalmonella enterica with the Use of Extremely Elevated Annealing Temperatures
... Modified thermal cycling conditions were explored in an effort to improve the reproducibility and resolving power of repetitive-element PCR (rep-PCR) fingerprinting. Assay performance was rigorously ...
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Improved Repetitive-Element PCR Fingerprinting for Resolving Pathogenic and Nonpathogenic Phylogenetic Groups withinEscherichia coli
... In the present study we rigorously evaluated the impact of radically modified thermal cycling conditions on the reproduc- ibility, resolving power, and phylogenetic validity of rep-PCR fingerprinting for E. ...
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Specific Primers for Rapid Detection of Microsporum audouinii by PCR in Clinical Samples
... Multiplex PCR with universal ...MSP-PCR fingerprinting, pro- duced the 431-bp fragment when tested with the MA1 ...for PCR fin- gerprinting and the M. audouinii-specific PCR, parameters ...
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Genomic fingerprints of Escherichia coli strains isolated from surface water in Alborz province, Iran
... One hundred E. coli were isolated from 100 water sam- ple indicating high fecal pollution of river. The genomic diversity analysis of 100 isolates of E. coli has been car- ried out with the use of the ERIC-PCR ...
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Genotypic and phenotypic diversity of Bacillus spp isolated from steel plant waste
... tDNA-PCR fingerprinting gave 10 and 11 distinct pat- terns, respectively ...genomic fingerprinting has been used in many organisms to infer phylogenetic relationships among iso- lates, and to study ...
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Comparison of four molecular typing methods for evaluating genetic diversity among Candida albicans isolates from human immunodeficiency virus positive patients with oral candidiasis
... and PCR fingerprinting with the phage M13 core sequence were performed with 30 isolates from the oral cavities of 30 human immunodeficiency virus (HIV)-infected patients and 8 reference ...
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Rapid identification and fingerprinting of Candida krusei by PCR based amplification of the species specific repetitive polymorphic sequence CKRS 1
... A PCR method was developed to identify and fingerprint Candida krusei isolates simply and ...and fingerprinting ability of the PCR assay were ...a PCR product with these primers. This ...
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Molecular typing of Salmonella typhi strains from Dhaka (Bangladesh) and development of DNA probes identifying plasmid encoded multidrug resistant isolates
... by PCR fingerprinting with primer RAPD1 and the amplification of extrachromosomal DNA, which is present exclusively in the MDR strains, we isolated four MDR-specific amplicons (amplicons A to D) ...
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DETECTION OF VIRULENCE GENES(EAE,BLACMY 2,BOX) IN ESCHERICHIA COLI ISOLATES FROM BETA THALASEMIC AND NON THALASEMIC PATIENTS BY USING PCR TECHINQUE
... BOX-PCR fingerprinting is applicable for typing of Ecoli isolates that can be grouped according to the BOX-PCR to 5 different genotypes; being their similarity (cut off point) were 20% as it depicted ...
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Transmission Dynamics of Tuberculosis in a High Incidence Country: Prospective Analysis by PCR DNA Fingerprinting
... mixed-linker PCR fingerprinting as described previously ...first PCR only fragments containing the 3⬘ end of IS6110 are amplified with a primer compatible with a 20-bp fragment at the 3⬘ end of ...
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Genetic diversity analysis of azotobacter in cotton soil of Yangtze River Basin
... the PCR amplification of denaturation at 95°C 7 min; remove PCR tubes quickly added ...the PCR amplification mix instrument , 30 cycles (94°C 1 min; 52 °C 1 min; 65 °C 8 min; 65 °C 16 min); 4 °C ...
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Rapid identification of Candida species by DNA fingerprinting with PCR
... by PCR fin- gerprinting prompted us to investigate whether this technique could be used to identify species of unknown clinical yeast ...pattern. PCR fingerprints indicated that all three isolates were a ...
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Incidence of Prevotella intermedia andPrevotella nigrescens Carriage among Family Members with Subclinical Periodontal Disease
... of PCR ribotyping and arbitrarily primed PCR (AP-PCR) fingerprinting and applied this system to the study of intrafamilial incidence of these species in the oral ...cavity. PCR ...
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Genetic Diversity of Rhizobial isolates revealed by PCR-RAPD fingerprinting and the data were analyzed using POPGEN.
... PCR fingerprinting of DNA enables the amplification of characteristic fingerprints of DNA bands from bacterial genomes. The precision and reproducibility of these fingerprints allow perfect discrimination ...
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Characterization of Leptospira isolates from serovar hardjo by ribotyping, arbitrarily primed PCR, and mapped restriction site polymorphisms
... Leptospira serovar hardjo isolates of the hardjoprajitno and hardjobovis genotypes were characterized by ribotyping, arbitrarily primed PCR AP-PCR fingerprinting, and the study of mapped[r] ...
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PCR based random amplified polymorphic DNA fingerprinting of Yersinia pseudotuberculosis and its practical applications
... Eight kinds of arbitrary primers shown in Table 2 and three representative Y pseudotuberculosis type strains, H655 lanes 1, Pa3433 lanes 2, and H123 lanes 3, were used for the RAPD tests[r] ...
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