PCR method
Multiplex PCR Method for Species Identification of Coagulase Positive Staphylococci
... The second reason is the public health issue of whether methicillin-resistant staphylococcal isolates from pet and farm animals are S. aureus strains. The isolation of methicillin-re- sistant S. aureus (MRSA) strains is ...
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A Simple LDT PCR Method for Detection of Various Nucleic Acid Sequence Changes in a Small Region of Genes—Application for the Identification of Gene Mutations in MTB rpoB Gene Associated with Drug Resistance
... temperature PCR (LDT-PCR) method combined with DNA microarray technique is deve- loped in our lab for quick and effective identification of various mutations in an 81 base pair region of ...
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Genotyping of Intron 22 and Intron 1 Inversions of Factor VIII Gene Using an Inverse-Shifting PCR Method in an Iranian Family with Severe Haemophilia A
... water. PCR was performed in a volume of 25 µl containing 3 µl and 6 µl of circularized DNA for assessing Inv1 and Inv 22, respectively, ...10× PCR buffer with the following step: a denaturing at 94°C for 2 ...
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Real Time PCR Method for Detection of Zygomycetes
... Culture isolates. Thirty-two isolates of zygomycetes belonging to the genera Absidia, Apophysomyces, Conidiobolus, Cunninghamella, Mucor, Rhizopus, Rhi- zomucor, Saksenaea, and Syncephalastrum were obtained from the ...
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A gel based PCR method to differentiate sheeppox virus field isolates from vaccine strains
... The PCR amplicons of SPPV Denizli and SPPV Morocco vaccine (Romanian vaccine strain) were selected to pre- pare positive control plasmids, representing the field iso- lates and vaccine strains ...
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A quantitative PCR method for measuring absolute telomere length
... CRITICAL: All oligomers should be HPLC purified; long oligomers (>50 mers) have a high failure rate dur- ing synthesis; this means there will be multiple failed sequences which must be removed in order to maintain ...
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Evaluation of Aspergillus PCR Protocols for Testing Serum Specimens
... a PCR control consisting of ...real-time PCR (13) and by the commercially available MycAssay Aspergillus (Myconostica, Manchester, United ...and PCR amplification ...extraction method, DNA ...
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Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites
... walking method based on TAIL-PCR. This PCR method added a 20-cycle linear amplifica- tion step before the exponential amplification step to increase the concentration of the target ...this ...
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Detection and Genotyping of Human Rotavirus VP4 and VP7 Genes by Reverse Transcriptase PCR and Reverse Hybridization
... transcriptase PCR (RT-PCR) method for the amplification of rotavirus RNA and a reverse hybridization assay on a strip to detect amplimers and identify the specific G and P genotypes present in human ...
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A Comparison between Culture and Multiplex PCR for Detection and Identification of Shigella Species in Patients with Shigellosis from Isfahan Province in 2014-2015
... Multiplex PCR method and comparison with the culture method to detect Shigella species, as well as analysing the antibiotic resistance pattern in its different ...culture method and ...
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Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae
... Comparison of our results obtained by the PCR method using primers 1 and 2 and the DNA colony hybridization test Table 2 indicated that our PCR method can be an alternative to the DNA co[r] ...
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Long-Term Infection of Mongolian Gerbils with Helicobacter pylori: Microbiological, Histopathological, and Serological Analyses
... Bacterial strain. The bacterial strain used was H. pylori TK1402, isolated from gastric biopsy specimens of a patient with gastric ulcer and duodenal ulcer. It was determined by the PCR method that the ...
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Analyzing ligation mixtures using a PCR based method
... a PCR based method and resulting in amplification of the initial template, the Lig-PCR method is preferable over other sensitive but complicated methods ...Lig-PCR method would ...
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Development of a highly sensitive method for detection of JAK2V617F
... the method to detect JAK2V617F in other blood ...time PCR-based assay that combines molecular beacon probe and locked nucleic acid techniques detected JAK2V617F mutation in about 10% of healthy donors ...
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Identification of piggyBac mediated insertions in Plasmodium berghei by next generation sequencing
... Several PCR-based approaches have been used to identify the piggyBac insertion sites in Plasmodium falciparum and Plasmodium berghei, but all are tedious and ...inverse PCR method was used to ...
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Nested PCR detection of malaria directly using blood filter paper samples from epidemiological surveys
... With a large sample collection of blood filter papers from cross-sectional surveys in the Thailand and Myanmar, the FP-PCR method was found to drastically increase the detection sensitivity over ...
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CANINE PARVOVIRUS- AN INSIGHT INTO DIAGNOSTIC ASPECT
... real-time PCR is used for quantification of PCR product in reaction which can be used for estimation of viral load in ...time PCR (RT-PCR) has been used for the detection of CPV-2 DNA in ...
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Comparison of the Clinical Performance of PapilloCheck Human Papillomavirus Detection with That of the GP5+/6+ PCR Enzyme Immunoassay in Population Based Cervical Screening
... -PCR method with an enzyme immunoassay readout (GP5 ⴙ /6 ⴙ -PCR–EIA) for the detection of high-risk HPV (hrHPV) types by the use of cervical samples originating from women in a population-based by ...
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Serotyping of Adenoviruses on Conjunctival Scrapings by PCR and Sequence Analysis
... PCR-sequence method. (i) DNA extraction from prototypes and clinical spec- imens. A 500-ml sample of each of the prototype-infected cell suspensions and conjunctival scrapings extracted with MEM solution ...
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Classical and Molecular Methods for Evaluation of Chlamydia trachomatis Infection in Women with Tubal Factor Infertility
... About 10% to 20% of female infertility is asso- ciated with tubal factor infertility. Although C. trachomatis infection will effectively clear in most women, but infection persists in some and may ascend to the upper ...
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