Pcr Rflp
Identification of Human Cytomegalovirus pUL27 R233 point mutation using PCR-RFLP
... and PCR-RFLP ...(PCR). PCR program cycles were as follows: 95° for 5 minutes, 30 loops with 30 seconds at 95°, ...All PCR products were run on ...1ug PCR product was diluted in a ...
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Comparison of Microscopy and PCR-RFLP for detection of Anaplasma marginale in carrier cattle
... Examination of 50 microscopic fields showed 25.8% sensitivity and 99% specificity compared to 100% sensitivity and specificity of PCR-RFLP. With sole use of this method, 16 blood samples were identified as ...
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PCR RFLP Methodology to Identify Nocardia Isolates in Cuba
... biotypes. PCR-RFLP of the 65-kDa heat shock protein (HSP) gene provides a rapid, sensitive, and time and labor-efficient method for this ...VI. PCR-RFLP of the 65-kDa HSP gene could be very ...
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Genotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis
... as PCR-restriction fragment length polymorphism (PCR-RFLP) and random amplified polymorphic DNA-PCR are cheaper and have high discriminatory power and reproducibility ...
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Discrimination of Paederus fuscipes and Paederus littoralis by mtDNA-COI PCR-RFLP
... Results: Sequence analyses of the data obtained in this study showed significant DNA polymorphisms. There were 89 substitutions between COI sequences of the two species. The mtDNA-COI fragment comprises several useful ...
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Efficiency of PCR-RFLP and Species-specific PCR for the Identification of Meat Origin in Dry Sausages
... when pork was present in meat mixtures. Moreover, Pietsch and Waiblinger (2010) reported that CYTB amplicons of the pig, turkey and chicken are ampli- fied more efficiently than those of cattle and sheep, leading to ...
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Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review
... compared PCR-SSCP and PCR-RFLP in terms of sensitivity, efficiency, technical requirements, time, costs, and other related ...between PCR-RFLP and PCR-SSCP in terms of their ...
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Cutaneous leishmaniasis in French Guiana: revising epidemiology with PCR-RFLP
... The study population was the patients in consultation for a suspicion of leishmaniasis at the LHUPM and in the health centers of the interior (administratively at- tached to Cayenne General Hospital) between 1 January ...
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PCR - RFLP patterns for the differentiation of the Fusarium species in virtue of ITS rDNA
... of PCR-amplified rDNA (rRNA gene) sequences has been shown to be a suitable method for the taxonomic studies in Fusarium ...of PCR-RFLP based on the identification of Fusarium species have been ...
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ANALYSIS OF POLYMORPHISM OF CAST GENE AND CLPG GENE IN SHEEP BY PCR-RFLP METHOD
... of PCR-RFLP method. The PCR products were digested with MspI restriction enzyme for calpastatin gene and FaqI restriction enzyme for callipyge ...
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A comparison between CHROMagar, PCR-RFLP and PCR-FSP for identification of Candida species
... The chromogenic medium CHROMagar has been provided for both isolation and identification of Candida species, based on the pigmentation of the colonies with different colors, which is due to different enzyme activities in ...
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Development of a PCR-RFLP method to distinguish species within the Ilyonectria macrodidyma complex
... The PCR-RFLP procedure designed in this study was able to differentiate between these cryptic species using restriction analysis of the histone H3 ...
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Identification of resistant carboxylesterase alleles in Culex pipiens complex via PCR RFLP
... of PCR-RFLP shows that the ...the PCR-RFLP results, the dif- ferentiation of the phenotypes of the two mosquito types would have been ...
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Detection of Toxoplasma gondii in sheep and goats blood samples by PCR-RFLP in Urmia
... and similar strains of Toxoplasma which could infect sheep in the region. Because of importance of the parasite, its consequences in human being and economic loss in food animals it is advised to perform PCR in ...
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GROWTH HORMONE GENE IN IRAQI AND TURKISH AWASSI SHEEP USING PCR RFLP
... using PCR-SSCP. Gupta etal. (2007) used the PCR-SSCP analysis to investigate growth hormone polymorphism in Indian black banegal goats, while Bastos ...
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Quadruplex Real Time PCR Assay Using Allele Specific Scorpion Primers for Detection of Mutations Conferring Clarithromycin Resistance to Helicobacter pylori
... Scorpion PCR and negative by culture, 12 were positive by PCR-RFLP with concordant results for detection of mutations conferring resistance to ...Scorpion PCR also displayed an indication of ...
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Considerable Genetic Diversity of Trichomonas vaginalis Clinical Isolates in a Targeted Population in South of Iran
... RAPD-PCR, PCR-RFLP, multilocus sequence typing (MLST) and sequencing polymorphism with a variety of markers such as ITS and mi- crosatellite have been described (9-11, ...20-24). ...
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Comparative Typing of Campylobacter jejuni by Heat Stable Serotyping and PCR Based Restriction Fragment Length Polymorphism Analysis
... and PCR-RFLP types and variations in the RFLP types of strains within a single Penner serotype were also ob- ...the PCR-RFLP method is applicable to the typing and classification of NT ...
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Comparison of Three PCR-based Methods for Simplicity and Cost Effectiveness Identification of Cutaneous Leishmaniasis Due to Leishmania tropica
... For PCR-RFLP the reaction was carried out with the master mix in 25 µl of total reaction and based on the following conditions: initial denaturation at 94 °C for 3 min, followed by 38 cycles including ...
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Definitive Differentiation between Single and Mixed Mycobacterial Infections in Red Deer (Cervus elaphus) by a Combination of Duplex Amplification of p34 and f57 Sequences and Hpy188I Enzymatic Restriction of Duplex Amplicons
... Sequence analysis of the amplicons prior to digestion con- firmed the restriction patterns. Digestion of the TCCGA site in the 257-bp amplicons from M. avium subsp. avium yielded 79-bp and 178-bp fragments, whereas the ...
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