Peptide binding
The Peptide-Binding and ATPase Domains of Recombinant hsc70 Are Required To Interact with Rotavirus and Reduce Its Infectivity
... synthetic peptide that mimics this region (peptide KID) and antibodies to hsc70 block the infec- tivity of rotavirus but not its binding to the cell surface (20, ...branes, binding nascent ...
10
Statistical deconvolution of enthalpic energetic contributions to MHC peptide binding affinity
... the binding and are maintained irrespective of the sequence of the ...the peptide and therefore make energetic contributions independent of the peptide ...and peptide residues are all ...
15
Crystal structure of the putative peptide-binding protein AppA from Clostridium difficile
... suggested that polypeptides encompassing residues Gly31– Glu498 of CdAppA and Ser2–Glu502 of CdOppA would be soluble and compact. The corresponding coding sequence of CdAppA was amplified from C. difficile 630 ...
9
Identification of the N-terminal Peptide Binding Site of Glucose-regulated Protein 94
... of binding activity is not due to global misfolding of the mutant ...radicicol binding-induced conformational change ...the peptide saturation level of H125Y ( ⬃ ...preclude peptide ...
11
Structure-based prediction of protein-peptide binding regions using Random Forest
... the binding sites were studied in previous work (London, et ...The peptide-binding regions in proteins appear to be dominated with large and flatter pockets (Olmez and Akbulut, ...small ...
9
A Novel Predictive Technique for the MHC Class II Peptide-Binding Interaction
... II peptide–binding ...endogenous peptide (PDB code:1AQD) (12) has been elucidated by X-ray diffraction at a resolution of ...experimental binding data set (17) from annealing temperatures ...
6
High-Throughput Engineering and Analysis of Class II Mhc/Peptide Binding by Yeast Co-Display
... target peptide or not is not enough sometimes, we would also like to know how well they bind with each other so that we can understand which residue at which position will favor or inhibit the interaction and what ...
185
An effective and effecient peptide binding prediction approach for a broad set of HLA-DR molecules based on ordered weighted averaging of binding pocket profiles
... the peptide binding ...the binding core is an essential step for MHC II ligand/epitope prediction, and the TEPITOPE method clearly reveals the MHC II molecules ’ preferred amino acids at the P1 ...
11
Methods for Prediction of Peptide Binding to MHC Molecules: A Comparative Study
... shown binding motifs to be amongst the least accurate of all predictive ...of peptide binding to the class I mol- ecule HLA-A*0201 and reported that quantitative matrices have high specificity and ...
12
Structural analysis of leader peptide binding enables leader free cyanobactin processing
... test peptide ITACITFCAYDG (mimicking the core peptide and C-terminal macrocyclization signature of PatE) in the absence of leader predominantly yields species containing only one heterocycle (Figures 3A and ...
22
Evidence for successive peptide binding and quality control stages during MHC class I assembly
... a peptide-receptive state, with very few molecules being retained in the ER ...stable peptide-depen- dent assembly of MHC class I molecules and the presen- tation of endogenous antigens to cytotoxic T ...
5
Natriuretic peptide binding sites in the gills of the pouched lamprey Geotria australis
... Gill membrane protein (75 µg) was incubated in incubation buffer with 0.25 nmol l −1 iodinated peptide in the presence or absence of excess unlabelled rANP or C-ANF. The final incubation volume was 250 µl. ...
12
Amastin Peptide-Binding Antibodies as Biomarkers of Active Human Visceral Leishmaniasis
... In the present study, we also assessed the level of IgG sub- classes toward amastin signature peptide and showed that IgG3 has the highest absorbance in all three stages. Although in our study there are no ...
7
Peptide binding to cleaved CD31 dampens ischemia/reperfusion-induced intestinal injury
... CD31 is a 130-kDa transmembrane glycoprotein receptor also expressed by the other leukocytes, platelets, and endothelial cells. Transhomophilic engagement of CD31 raises the activation threshold of these cells via ...
12
Inhibition of Herpes Simplex Virus gD and Lymphotoxin-α Binding to HveA by Peptide Antagonists
... identified peptide ligands, while differing in pri- mary structure, each had two cysteine ...for peptide binding to ...gDt binding to HveAt when their two Cys residues were not disulfide ...
7
Synthesis, gallium labelling and characterization of P04087, a functionalized phosphatidylserine-binding peptide
... of peptide binding (whatever the tracer) at the peripheral layer of vegetations, corresponding to the recruitment of activated platelets from the blood pool ...
17
Binding Dynamics of Hepatitis C Virus' NS5A Amphipathic Peptide to Cell and Model Membranes
... AH peptide was injected (arrow 3) to investigate the ability of the AH peptide to bind to the model POPC ...AH peptide was indeed bound to the membrane, the latter was washed again with PBS buffer ...
8
Hotspot autoimmune T cell receptor binding underlies pathogen and insulin peptide cross reactivity
... weak binding affinity between 1E6 and A2- MVWGPDPLYV and A2-YLGGPDFPTI generated thermodynamic data that were not robust enough to gain insight into the enthalpic (ΔH°) and entropic (TΔS°) changes that contributed ...
15
Binding specificity and affinity analysis of an anti protective antigen peptide reagent using capillary electrophoresis
... αPA peptide (3 μM final concentration) was mixed with varying PA protein concentrations (0 - 3 μM) in a 20 μL total volume in 10 mM potassium Hepes pH ...the binding affinity due to residual PA or ...
6
Characterization of Calcium Binding Peptide Derived from Calreticulin and Its Effect on Increasing Bioavailable Calcium in Plants
... 2+ binding protein with 3 predicted EF-hands ...of binding Ca 2+ . The novel Ca 2+ -binding properties of SOS3 are likely to be important in determining the specificity of Ca 2+ signaling under Na + ...
165