Peptide mapping
Structure of the hepatitis A virion: peptide mapping of the capsid region.
... The anti-HAV peptide serum against each potential larger HAV capsid polypeptide located in the P1 genome region was reacted in an EITB with HAV polypeptides separated by 10 to 25% gradie[r] ...
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Chagas disease specific antigens: characterization of epitopes in CRA/FRA by synthetic peptide mapping and evaluation by ELISA peptide assay
... the mapping of linear epitope through parallel synthesis of overlapping peptides was chosen based on the volume of useful information provided and the high spatial resolution of the ...14-residue peptide, ...
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Purification, Peptide Mapping and Spectroscopic Characterization of Myoglobin from Striped Snake-Head Fish (Ophicephalusstriatus)
... partial peptide sequences of 15 kDa protein band expected to be the fish Mb, were obtained from LC-MS/MS with in-gel trypsin ...the peptide sequences were homologous with those of some ...partial ...
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BALB/c myeloma retroviruses: peptide mapping and immunological analysis of the pp12 structural protein.
... Logit transformation analysis of the data from competition RIA also demonstrated the similarity of FL-1 ppl2 to the ppl2 of ecotropic WN 1802 B virus and the fact that the MO-21 and FL-1[r] ...
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Immunogenic domains of hepatitis delta virus antigen: peptide mapping of epitopes recognized by human and woodchuck antibodies.
... In an effort to map the antigenic domains of HDAg, 209 overlapping hexapeptides, spanning the entire 214 amino acid residues of the protein, were synthesized on polyethylene pins and pro[r] ...
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Measles virus polypeptides in infected cells studied by immune precipitation and one-dimensional peptide mapping.
... Although such a cleavage has not been ruled out, the 32P labeling data indicate that the measles virus M protein undergoes phosphorylation in the infected cell and is in this respect ana[r] ...
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Akt1 sequentially phosphorylates p27kip1 within a conserved but non-canonical region
... Phospho-peptide mapping of p27 revealed three radiolabeled spots, two of which lie in a diagonal characteristic of the same peptide containing different amounts of phosphate ...tryptic peptide ...
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Synthesis of Sindbis virus nonstructural polypeptides in chicken embryo fibroblasts.
... 22, 1977 SINDBIS VIRUS NONSTRUCTURAL POLYPEPTIDES 421 in Sindbis virus-infected cells and show by tryptic peptide mapping and pulse-chase experiments that these polypeptides are synthesi[r] ...
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In vitro translation of avian myeloblastosis virus RNA.
... Based upon two-dimensional peptide mapping, the 56,000- and 48,000-dalton proteins did not appear to be related to Pr769'9, the MAV-1 env gene products, or the proteins produced by trans[r] ...
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Phosphorylation of murine mammary tumor virus precursor polypeptides.
... Tryptic peptide mapping analysis of the 32P-labeled viral proteins indicated a precursor product relationship between the intracellular phosphorylated, high-molecular-weight peptides and[r] ...
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Identification, synthesis, and modifications of simian rotavirus SA11 polypeptides in infected cells.
... V8 protease peptide mapping 4 ,ug/ml was used to compare the relationship of the glycoprotein from purified virus 38K*, the glycoprotein from infected cells 38K, the postulated precursor[r] ...
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Frequency of occurrence of novel milk protein variants in a New Zealand dairy cattle study population : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry, Massey University
... Polyacrylamide gel electrophoresis PAGE, free zone capillary electrophoresis CE, peptide mapping by reverse-phase HPLC and electrospray mass spectrometry ESI-MS were used in the characte[r] ...
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Primary structure study of phosphofructokinase from Streptococcus lactis : a thesis presented [in] fulfilment of the requirement for the Master of Science in biochemistry at Massey University
... Fi gure 10 SDS-PAGE of PFK isolation results Fi gure 11 Time-dependancy of amino acids during hydrolysis Figure 12 Tryptic peptide mapping on RPLC Fi gure 13 Purification of RPLC fractio[r] ...
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Primary structure study of phosphofructokinase from Streptococcus lactis : a thesis presented [in] fulfilment of the requirement for the Master of Science in biochemistry at Massey University
... Fi gure 10 SDS-PAGE of PFK isolation results Fi gure 11 Time-dependancy of amino acids during hydrolysis Figure 12 Tryptic peptide mapping on RPLC Fi gure 13 Purification of RPLC fractio[r] ...
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The Pfs230 N terminal fragment, Pfs230D1+: expression and characterization of a potential malaria transmission blocking vaccine candidate
... Correct disulfide bond formation within recombi- nant proteins is important to maintain native epitopes and ensure induction of functional antibodies; herein evidence was provided that the appropriate intramo- lecular ...
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Thermal plasticity of skeletal muscle phenotype in ectothermic vertebrates and its significance for locomotory behaviour
... In contrast to temperature acclimation in common carp and goldfish, two-dimensional gel electrophoresis and peptide mapping of purified fast muscle myosin heavy chains revealed no myofib[r] ...
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Synthesis and processing of the envelope gp55-116 complex of human cytomegalovirus.
... 58, 1986 using SYNTHESIS OF CMV ENVELOPE gp55-116 COMPLEX one-dimensional proteolytic peptide mapping of the mature virion forms of these proteins also indicate that gp55 ACKNOWLEDGMENTS[r] ...
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ML antigen of DBA/2 mouse leukemias: expression of an endogenous murine mammary tumor virus.
... Isolation of MuMTV core protein p28 from the leukemic cells and subsequent tryptic peptide mapping analysis showed that the p28 from leukemia cells differed from the p28 of MuMTV isolate[r] ...
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Collagen from Tendon of Yezo Sika Deer (Cervus nippon yesoensis) as By Product
... Peptide Mapping of Collagen from Tendon of Yezo Sika Deer By SDS-PAGE using 10% gel, the denatured acid-soluble and pepsin-solubilized collagens from tendon of Yezo sika deer were invest[r] ...
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Common Precursor for Rauscher Leukemia Virus gp69/71, p15(E), and p12(E)
... This sharply contrasted with our tryptic mapping data la, which clearly showed that a p12 protein [pl2E], isolated by SDS-PAGE, shared a methionine-containing tryptic peptide fraction wi[r] ...
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