Peripheral Blood Mononuclear Cells
Aberrant splicing of the hRasGRP4 transcript and decreased levels of this signaling protein in the peripheral blood mononuclear cells in a subset of patients with rheumatoid arthritis
... mast cells; PM/DM: polymyositis/ dermatomyositis; PBMCs: peripheral blood mononuclear cells; PBMCs: peripheral blood mononuclear cells; RasGRP: Ras guanine ...
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Regulatory Effects of Estradiol on Peripheral Blood Mononuclear Cells Activation in Patients with Asthma
... Figure 2. Effect of E2 on the expression of IL-6, IL-17, IL-23 and TGF-β in patients with asthma (n=17) and healthy individuals (n=20). PBMCs culture were established in three experimental groups and one group as ...
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Ion Channels in Peripheral Blood Mononuclear Cells – Effect of Culture and Ascorbic Acid.
... isolated mononuclear cells on exposure to ...which peripheral blood mononuclear cells were cultured with LPS in the presence of vitamin C and vitamin E showed a decrease in PGE2 ...
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<em>USP18</em> downregulation in peripheral blood mononuclear cells predicts nonresponse to interferon-based triple therapy in patients with chronic hepatitis C, genotype 1: a pilot study
... Patients and methods: Expression of IFNG, IFNLR1, and interferon-sensitive genes CXCL9, IFI16, IFI27, ISG15, and USP18 in peripheral blood mononuclear cells was assessed before and during the ...
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Molecular Profile of Peripheral Blood Mononuclear Cells from Patients with Rheumatoid Arthritis
... Rheumatoid arthritis (RA) is a chronic inflammatory arthritis. Currently, diagnosis of RA may take several weeks, and factors used to predict a poor prognosis are not always reliable. Gene expression in RA may consist of ...
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VPAC1 receptor expression in peripheral blood mononuclear cells in a human endotoxemia model
... Venous blood samples were collected at baseline, 3 hours, 6 hours and 24 hours after LPS administration for peripheral blood mononuclear cells (PBMC) isolation and VIP serum level ...
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Ethyleneglycol-Bis-(β-Aminoethylether)Tetraacetate as a Blood Anticoagulant: Preservation of Antigen-Presenting Cell Function and Antigen-Specific Proliferative Response of Peripheral Blood Mononuclear Cells from Stored Blood
... in peripheral blood mononuclear cells (PBMC) obtained from blood exposed for prolonged periods to EDTA as an ...whole blood during storage in EDTA or to lymphocytes from ...
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Effect of Contaminating Red Blood Cells on OKT3-Mediated Polyclonal Activation of Peripheral Blood Mononuclear Cells
... studied cells of the hematopoietic system because of their central role in the generation of immune ...ripheral blood, lymphoid tissues, and certain internal ...ripheral blood compartment and ...
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p66Shc gene expression in peripheral blood mononuclear cells and progression of diabetic complications
... PAD: peripheral arterial disease; PBMC: peripheral blood mononuclear cells; PBS: phosphate buffer saline; PCR: polymerase chair reaction; Shc: Src-homology collagen; UACR: urinary ...
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Activation of Peripheral Blood Mononuclear Cells by Dengue Virus Infection Depotentiates Balapiravir
... dendritic cells, spreads to lymph nodes, and disseminates to various tissues and ...in peripheral blood mononuclear cells (PBMCs) were reported to be major rep- lication sites in ...
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Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2).
... human peripheral blood mononuclear cells (PBMC), and ii) human hepatoma cell line (HepG2), respectively, to evaluate its direct effects and after liver enzymes ...
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Functional Measurement of Hepatitis C Virus Core Specific CD8+ T Cell Responses in the Livers or Peripheral Blood of Patients by Using Autologous Peripheral Blood Mononuclear Cells as Targets or Stimulators
... the peripheral blood and because the targeted epitopes vary depending on the human leukocyte antigen (HLA) types of the ...autologous peripheral blood mononuclear cells (PBMCs) ...
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Gene expression signatures for autoimmune disease in peripheral blood mononuclear cells
... experimental systemic lupus erythematosus (SLE) [10]. In human autoimmunity, biopsy samples from tissues such as rheumatoid synovium [6,9] and skin [7] have yielded disease insights. Other groups of investigators have ...
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Viability and Functionality of Cryopreserved Peripheral Blood Mononuclear Cells in Pediatric Dengue
... 6 cells were washed and resuspended in 1 ml of sterile 1 ⫻ Dulbecco’s phosphate-buffered saline (DPBS) (catalog ...the cells were washed with 3 ml of fluorescence-activated cell sorter (FACS) buffer ...
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Ex Vivo Stimulation and Expansion of both CD4+ and CD8+ T Cells from Peripheral Blood Mononuclear Cells of Human Cytomegalovirus-Seropositive Blood Donors by Using a Soluble Recombinant Chimeric Protein, IE1-pp65
... dendritic cells (for a review, see reference ...dendritic cells have the capacity to deliver exog- enous Ag in the cytosolic pathway, as we recently demon- strated using pp65-positive apoptotic bodies (2), ...
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Exploring peripheral blood mononuclear cells as the source of interleukin 6 in polymyalgia rheumatica
... Despite being the subject of many years of research, the cellular source of IL-6 in PMR and the causative agent of its release are yet to be identified. A few isolated studies have shown limited evidence for the ...
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Platelet-activating factor changes in phospholipid extracts from the plasma, peripheral blood mononuclear cells and bone marrow mononuclear cells of patients with acute leukemia — A 31P MRS in vitro study
... 19. Donnard, M., Guglielmi, L., Turlure, P., Piguet, C., Couraud, M.J., Bordessoule, D. and Denizot, Y. Membrane and intracellular platelet- activating factor receptor expression in leukemic blasts of patients with acute ...
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Donor variability in HIV binding to peripheral blood mononuclear cells
... to cells independently of CD4 ...dendritic cells through syn- decan-3 and a variety of C-type lectin receptors [9-11], and HeLa cells through cell-surface heparans ...donor cells, we ...
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The multi-differentiation potential of peripheral blood mononuclear cells
... positive cells, suggesting no cell membrane fusion between trans- planted cells and host ...CD34+ cells into the infarcted myocardium of SCID ...donor cells, double staining for HLA and SMA ...
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Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation
... mechanism is based on intracellular-ice formation by the reduction temperature of water in the cells without any cryogenic damages. This could effectively stop cell movement and shut down the cell biochemical ...
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