phage display
Nanoscale bacteriophage biosensors beyond phage display
... Abstract: Bacteriophages are traditionally used for the development of phage display technology. Recently, their nanosized dimensions and ease with which genetic modifications can be made to their structure ...
9
Exploration of the HIF-1α/p300 interface using peptide and Adhiron phage display technologies.
... second phage display experiment used non-antibody binding proteins presented on the surface of the phage as opposed to short peptides; such scaffolds are designed to constrain and present variable ...
12
Screening and selection of peptides specific for esophageal cancer cells from a phage display peptide library
... peptide phage display technology can select for peptide ligands that have high specificity and affinity for cell surface proteins in both in vitro and in vivo ...peptide phage display library ...
7
Isolation of isoform specific binding proteins (Affimers) by phage display using negative selection
... by phage display ...other phage display libraries, including the Affimer library; however this method does not describe a strategy to deselect against homologous ...
28
Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display
... Phage display isolates both pathogenic and nonpathogenic scFv antibodies. ScFv polypeptides consist of a single monovalent variable-region antigen-binding site. To evaluate the ability of anti-Dsg scFvs to ...
13
Screening and identification of a renal carcinoma specific peptide from a phage display peptide library
... often display certain cell surface antigens such as tumor-associated antigens or tumor-specific antigens in high quantity, which are different from the antigens on normal ...peptide phage display ...
6
Screening and Identification of a Targeting Peptide to Hepatocarcinoma from a Phage Display Peptide Library
... a phage display li- brary provides a powerful tool to obtain the specific binding peptides through the selection of desired binding properties ...complex phage li- braries have been reported to yield ...
9
Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library
... The phage display from Ph.D.-7 library gave the consensus results. The resultant peptide sequence with the most redundancy may be the most favorable ligand for Hsp40 Ydj1. There are the multiple DNA ...
5
Phage display derived inhibitor of the essential cell wall biosynthesis enzyme MurF
... Background: To develop antibacterial agents having novel modes of action against bacterial cell wall biosynthesis, we targeted the essential MurF enzyme of the antibiotic resistant pathogen Pseudomonas aeruginosa. MurF ...
12
Phage display selected magnetite interacting Adhirons for shape controlled nanoparticle synthesis
... This work was supported by funding from the BBSRC (BB/H005412/2) and EPSRC (EP/L000202). Additional funding was from The University of Leeds via the Biomedical and Health Research Centre and The University of ...
9
Phage display selected magnetite interacting Adhirons for shape controlled nanoparticle synthesis
... This work was supported by funding from the BBSRC (BB/H005412/2) and EPSRC (EP/L000202). Additional funding was from The University of Leeds via the Biomedical and Health Research Centre and The University of ...
9
SAROTUP: a suite of tools for finding potential target-unrelated peptides from phage display data
... in phage display ...sequencing phage display data and distributed it as an open source package, which can perform perfectly locally on almost all systems that support C++ with little or no ...
8
Joining the in vitro immunization of alpaca lymphocytes and phage display: rapid and cost effective pipeline for sdAb synthesis
... sdAb phage library, selection of antigen specific sdAb expressed on phages (biopanning) and confirmation of binding ability of sdAb to the ...the phage display ten unique phage clones carrying ...
13
Human Neutralizing Fab Molecules against Severe Acute Respiratory Syndrome Coronavirus Generated by Phage Display
... Construction of a Fab phage display library. Total RNA was extracted from peripheral blood lymphocytes of convalescent SARS patients (RNA isolation kit; Stratagene), and mRNA was reverse transcribed into ...
5
Mimotopes of the Vi Antigen of Salmonella enterica Serovar Typhi Identified from Phage Display Peptide Library
... In summary, we have demonstrated in this study that it is possible to identify peptide mimotopes of disease-related im- munodominant carbohydrate epitopes from a phage display peptide library by using ...
7
Phage display library selection of a hypoxia-binding scFv antibody for liver cancer metabolic marker discovery
... Phage display technology can be used to isolate a specific antibody (Ab) able to bind its cognate antigen or to internalize into tumor cells ...Ab phage display, Ab fragments, corresponding to ...
17
Induction of anti-EGFR immune response with mimotopes identified from a phage display peptide library by panitumumab
... cells expressing high levels of EGFR by MTT and colony formation assays. The results of MTT assays suggested that antibodies induced by Hsc70-P19 and Hsc70-P26 significantly inhibited the growth of A431 (Figure 3D upper ...
14
Phage Display of Adenovirus Type 5 Fiber Knob as a Tool for Specific Ligand Selection and Validation
... of phage-displayed ligand ...high-throughput phage library screening with in-context ligand functional and structural suit- ...amentous-phage display allows only amino-terminal insertions into ...
7
Phage display and bacterial expression of a recombinant Fab specific for Pseudomonas aeruginosa serotype O6 lipopolysaccharide.
... using phage display and cloning of these Ig genes into a chimeric vector system designed to add a human constant region to the variable region genes, thus minimizing the murine components of these ...
9
Production and Evaluation of Antibodies and Phage Display Derived Peptide Ligands for Immunomagnetic Separation of Mycobacterium bovis
... Immunogen preparation. Polyclonal and monoclonal antibodies were prepared using whole-cell suspensions or ethanol-extracted surface anti- gens. Mycobacterium bovis AF2122/97 (provided by M. Vordermeier, Vet- erinary ...
8