PI: propidium iodide
Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells
... IVD: intervertebral disc; NP: nucleus pulposus; NPC: nucleus pulposus cells; NPPC: nucleus pulposus progenitor cells; PI: propidium iodide; P/S: penicillin/ streptomycin; PLGA: Poly (lac[r] ...
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Visualization of internalization of functionalized cobalt ferrite nanoparticles and their intracellular fate
... Notes: Cells were incubated with increasing concentrations of functionalized NPs for 24 hours. The standard MTS viability assay was performed; for the PI viability assay, cells were incubated for 5 minutes with ...
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Inhibition of NF-kappaB transcriptional activity enhances fucoxanthinol-induced apoptosis in colorectal cancer cells
... (propidium iodide), as described by the Tali® Apoptosis Kit – Annexin V Alexa Fluor® 488 and propidium iodide (Life Technolo- gies Corporation, Van Allen Way Carlsbad, CA, ...
8
Pathogen- and Host-Directed Antileishmanial Effects Mediated by Polyhexanide (PHMB)
... using propidium iodide (PI), a dye that can enter the parasite and stain DNA only after loss of mem- brane ...by PI and staining increased at later time points (Fig ...
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Fluorometric Viability Assessment of Capacitated and Acrosome Reacted Boar Spermatozoa by Flow Cytometry
... Sperm capacitation involves functional changes, such as the removal or appearance of specific molecules and changes in the plasma membrane; the acrosome reaction (AR) is an exocytotic event induced by calcium influx, ...
5
Cellular apoptosis: An alternative mechanism of action for caspofungin against Candida glabrata
... V/propidium iodide (PI) kit according to the manufacturer’s instruction (eBioscience, ...and PI, sample acquisition was performed using the Partec flow cytometry ...
7
Evaluation of Stress Response and Apoptosis on Leucocytes in TIVA versus Balanced Anesthesia
... Propidium Iodide staining (PI) was used with Annexin V. The PI is a mem- brane impermeable DNA stain, which is often used to differentiate between early and late ...
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Anticancer phototherapy using activation of E combretastatins by two photon–induced isomerization
... Combretastatin induced apoptosis on CHO cell monolayers was determined using Annexin V AlexaFluor 488 conjugate (Invitrogen) as an indicator for the loss of phospholipid asymmetry in the plasma membrane of apoptotic ...
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In Vitro Toxicity Evaluation of Caffeine Imprinted Polymer (CAF-MIP) for Decaffeination Method on Normal Chang Liver Cells
... propidium iodide (AO/PI) staining for mode of cell death observation.Proliferation assay was also conducted after 24, 48 and 72 hours at 30 µg/ml on NCLC and it showed that CAF-MIP promote NCLC ...
7
Original Article The role of parthenolide in the inhibition of proliferation and regulation of apoptosis of the human cervical carcinoma HeLa cells
... We used propidium iodide PI staining and flow cytometry [5-7] to deter- mine apoptosis and cell cycle changes caused by parthenolide in human cervical cancer HeLa cells; the levels of cy[r] ...
7
SOX7 is down-regulated in lung cancer
... 2x10 6 cells stably expressing either SOX7 or GFP were seeded into 6-well plates for 24 h. Cells were harvested and washed twice with cold phosphate- buffered saline (PBS) and fixed in 75% ethanol (precooled at -20°C) ...
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Repurposing of nitroxoline as a potential anticancer agent against human prostate cancer – a crucial role on AMPK/mTOR signaling pathway and the interplay with Chk2 activation
... Inhibition of cyclin D1-Rb-Cdc25A axis contributes to nitroxoline-induced G1 arrest Flow cytometric analysis of propidium iodide PI staining in PC-3 cells revealed that nitroxoline induc[r] ...
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Localization of Single- and Low-Copy Sequences on Tomato Synaptonemal Complex Spreads Using Fluorescence in Situ Hybridization (FISH)
... Fluorescence in situ hybridization (FISH) is a powerful means by which single- and low-copy DNA sequences can be localized on chromosomes. Compared to the mitotic metaphase chromosomes that are normally used in FISH, ...
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The Coronavirus Endoribonuclease Nsp15 Interacts with Retinoblastoma Tumor Suppressor Protein
... The cells were transfected to express sNsp15HA, LCHA, or H249AHA the endoribonuclease mutant of sNsp15 and stained to detect annexin V and DNA with propidium iodide PI.. For data analysi[r] ...
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Cell-permeable iron inhibits vascular endothelial growth factor receptor-2 signaling and tumor angiogenesis
... experiments. These results confirm that cell permeable iron, FAC, inhibits VEGF-induced endothelial cell proliferation. In order to investigate the mechanism of inhibition of VEGF-A induced cellular proliferation, we ...
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The effect of piperlongumine on endothelial and lung adenocarcinoma cells with regulated expression of profilin-1
... The cell death in both cell lines was investigated using Guava easyCyte 6HT-2L Benchtop Flow Cytometer (Merck Millipore, Darmstadt, Germany) and double staining with Annexin V Alexa Fluor 488 (AV)/propidium ...
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Abstract
... Background. The MTT assay is widely recommended for examining the cytotoxic effect of xenobiotics, assessing proliferation rates, and analyzing cell activity. The aim of this study was to evaluate the usefulness of the ...
5
A novel small-form NEDD4 regulates cell invasiveness and apoptosis to promote tumor metastasis
... Stable cells were treated in the presence or absence of low -serum conditions medium with 1% serum for 48 h, followed by Annexin V-propidium iodide PI double staining, and the proportion[r] ...
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A comparative assessment of antiproliferative properties of resveratrol and ethanol leaf extract of Anogeissus leiocarpus (DC) Guill and Perr against HepG2 hepatocarcinoma cells
... Cells were harvested and re-suspended in medium to ensure a single cell suspension and then counted on a haemocytometer based on trypan blue dye exclusion method (0.4% trypan blue; Sigma-Aldrich, St Louis, MO) by adding ...
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TGF-β1 Increases Resistance of NIH/3T3 Fibroblasts Toward Apoptosis Through Activation of Smad2/3 and Erk1/2 Pathways
... with propidium iodide and increased annexin V binding (Figure 3A), indicative of ...of propidium iodide- and annexin V-positive cells was ...propidium iodide. Pretreatment of ...
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