Plaque Reduction
Varicella zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique
... A new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed usin[r] ...
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Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK 21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization
... We recently reported a simplified plaque reduction neutralization test PRNT adapted to semimicro methods in LLCMK2 cells that was comparable in sensitivity and specificity to the standar[r] ...
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Comparison of commercial enzyme immunoassay kits with plaque reduction neutralization test for detection of measles virus antibody
... Four commercially available enzyme immunoassay (EIA) kits were evaluated in comparison with the plaque reduction neutralization (PRN) test for detection of measles virus antibody. The EIA kits, Enzygnost ...
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Evaluation of Chimeric Japanese Encephalitis and Dengue Viruses for Use in Diagnostic Plaque Reduction Neutralization Tests
... The plaque reduction neutralization test (PRNT) is a specific serological test used to identify and confirm arbovirus infection in diagnostic laboratories and monitor immunological protection in vaccine ...
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Development of a Novel Efficient Fluorescence-Based Plaque Reduction Microneutralization Assay for Measles Virus Immunity
... fluorescence-based plaque reduction microneutralization (PRMN) assay, using recombinant MV based on the Edmonston B strain, engineered to express en- hanced green fluorescent protein, MVeGFP (9, ...
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Variation in dengue virus plaque reduction neutralization testing: systematic review and pooled analysis
... Background: The plaque reduction neutralization test (PRNT) remains the gold standard for the detection of serologic immune responses to dengue virus (DENV). While the basic concept of the PRNT remains ...
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Antiviral activity evaluation of some pyrimidine derivatives using plaque reduction assay
... Preparing stock solutions of pyrimidine derivatives and antiviral drugs The first and second compounds were dissolved in the 5 mg/ml and 2 mg/ml sodium bicarbonate solution, respectively[r] ...
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Serotypic classification of hantaviruses by indirect immunofluorescent antibody and plaque reduction neutralization tests
... Antisera prepared against 16 strains of hantaviruses isolated from patients with hemorrhagic fever with renal syndrome HFRS or from rodents captured in HFRS-endemic and nonendemic region[r] ...
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Analysis by plaque reduction neutralization assay of intertypic rotaviruses suggests that gene reassortment occurs in vivo
... In the present study we have extended our examination of the antigenic specificities of VP3 by analyzing two additional, previously described 10, naturally occurring intertypic rotavirus[r] ...
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Efficacy of chewable brush compared to manual brush in school going children of age group 10 to 12 years
... Quigley-Hein Plaque Index (TMQHPI) ...the plaque removal efficacy of the respective ...TMQHPI plaque reduction compared to baseline ...in plaque removal but the efficacy of powered ...
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Reactivity of VP4 specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human (symptomatic and asymptomatic) and animal rotaviruses
... The reactivity of these MAbs with distinct serotypes of animal rotaviruses and symptomatic and asymptomatic human rotaviruses was examined by a plaque reduction virus neutralization PRVN[r] ...
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Antiviral and virucidal activities of Duabanga grandiflora leaf extract against Pseudorabies virus in vitro
... infection via the cytopathic effect, plaque reduction, viral replication, and virucidal activities. However, the anti- viral activities of the D. grandiflora leaf extracts are dependent on the solvent used ...
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Detection of Rift Valley fever virus antigen by enzyme linked immunosorbent assay
... Since vaccine antigens responsible for stimulation of RVF antibodies may not be detectable during plaque reduction neutralizing antibodies are not the fiirst few days of disease and sinc[r] ...
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Radiometric cytolysis inhibition assay, a new rapid test for neutralizing antibodies to intact and trypsin cleaved poliovirus
... The RACINA, carried out at 30°C overnight, was found to be suitable for measuring antibodies to trypsin-cleaved poliovirus and capable of replacing the cumbersome plaque reduction assay [r] ...
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Anti viral activity of culinary and medicinal mushroom extracts against dengue virus serotype 2: an in vitro study
... significant reduction in the expression levels of the ENV ...the plaque reduction assay, infected cells treated with ASEs showed a higher reduction in the expression of the NS5 and ENV genes ...
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Rapid quantification and neutralization assays for novel coronavirus SARS-CoV-2 using Avicel RRC-591 semi-solid overlay
... of plaque counts between multiple analysts (a) Three analysts independently counted 72 samples and the individual counts were plotted against the mean of the three ...of plaque reduction and compared ...
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Antiviral and Antimicrobial Activity of Thymus transcaspicus Essential Oil
... In the present study, the antiviral and antimicrobial effects of Thymus transcaspicus essential oil were investigated. Plant essential oil was prepared by a hydro-distillation method using Clevenger-type apparatus. ...
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Use of transdominant mutants of the origin-binding protein (UL9) of herpes simplex virus type 1 to define functional domains.
... We constructed two mutants (L12 and L34) in which the leucine zipper region was disrupted by the substitution of leucine residues in positions 1 and 2 and positions 3 and 4, respectively) with valine residues (31). If ...
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Vesicular stomatitis virus plaque production in monolayer cultures with liquid overlay medium: description and adaptation to a one day, human interferon plaque
... WISH amnion cells also are highly sensitive to the antiviral effects of human interferon, and a quantitative human interferon assay, based on vesicular stomatitis virus plaque reduction [r] ...
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Euphorbia Microsciadia Percolation and Soxhlet Extracts Exhibit Antiviral Activity
... Therefore, in this study the antiviral effects of Euphorbia microsciadia extracts (EME) were investigated using a plaque reduction assay. After collecting and authentication, aerial parts of the plant were ...
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