Plasmid sizes and calculated copy number
Plasmid Copy Number Control: Isolation and Characterization of High-Copy-Number Mutants of Plasmid pe194
... Comparison of radioactivity present as covalently closed circular DNA and in the main chromosomal DNA band yielded the following estimates of copy number: 10, pE194; 10, pE194 tyl-1; and[r] ...
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Escherichia coli Plasmid Copy Number Assay
... of plasmid copy number can be achieved using sequence-specific DNA assays on the Threshold ® ...System. Plasmid copy number is essentially the number of copies of a given ...
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Sociobiological Control of Plasmid Copy Number in Bacteria
... low copy numbers can be effectively maintained even without prevention of ...low copy number conjugal plasmids should confer immunity to the host against conjugal invasion whereas high copy ...
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Plasmid Copy Number Underlies Adaptive Mutability in Bacteria
... the plasmid lac operon. Cells might acquire multiple plasmid copies in several ...of plasmid copy-number control could lead to occasional cells with mul- tiple independent ...
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Modelling the Segregation Mechanism of low copy number Plasmid pB171
... pB171 plasmid partitioning ...a plasmid, we investigated the idea that ParA subunits might pull the plasmid in a similar way as the model in which filaments pull with drag included, but instead of ...
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PLASMID STABILITY AND MAINTENANCE OF COPY NUMBER USING NATURAL MARKER
... the plasmid. On successive generations there is a chance of the reducing copy number and thereby affecting the production of ...the plasmid is also a major concern, due to which the usage has ...
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Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes
... for plasmid copy number deter- mination have been described in the ...indirect plasmid copy number (PCN) determina- tion, by measuring the activity of the reporter protein coded ...
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Control of Plasmid Ri Replication: Functions Involved in Replication, Copy Number Control, Incompatibility, and Switch-off of Replication
... The resulting chimeric plasmids, pKN388 (parent plasmid pKN104) and pKN431 (parent plasmid pKN102), were tested for switch-off of replication functions and incompatibility prop-. erties [r] ...
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This is a repository copy of Comparative study of calculated and actual dimensions in shaped weft-knitwear.
... Table 3. Using two different empirical methods to calculate knitted armhole shapes. After the initial experiment, two higher fashion frequencies were explored (6 and 8 courses), the raglan method was compared with a new ...
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Ty1 Copy Number Dynamics in Saccharomyces
... the other underwent two events, conversion of URA3 charomyces (Wilke et al. 1992; Neuveglise et al. 2002), by Ty1 DNA or cDNA and a separate Ty1HIS3 insertion including complete loss in S. paradoxus strain 337. elsewhere ...
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Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum
... high-copy plasmid, we first adopt GST as a small size cytoplasmic protein and also chose α-amylase as a large size secretory pro- ...high-copy plasmid pCB4270. Cells harboring each ...
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CiteSeerX — Functional Copy-Number Alterations in Cancer
... the copy number assigned to its parental segment, which maintains the correlation in copy ...event sizes, allowing for a permutation of copy number values and not positions, ...
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The copy number of bacterial plasmids often is regulated by
... of plasmid P1 contains an array of five repeats (iterons) that bind the plasmid-encoded initiator ...on plasmid replication would titrate RepA and release the ...P1 copy number is ...
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Single cell copy number variation detection
... window sizes improves the genome composition correction, we also performed the correction based only on the GC content of each probe, as proposed by the CGprobeA ...
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Copy Number Heterogeneity of JC Virus Standards
... relative copy number of JC virus (JCV) clini- cal ...the copy number differences nor the rearrangements were observed in a clinical sample with a high copy number of JCV or a ...
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Copy number variations and cancer
... on copy number variations (CNVs), an unexpectedly frequent, dynamic and complex form of genetic diversity, and have quickly overturned the idea of a single diploid human ‘reference ...
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COPY NUMBER CONTROL OF Tn5 TRANSPOSITION
... SupB-suppressed ISSOL proteins are synthesized at about 20% the rate of IS5OR proteins (R. REZNIKOFF, unpublished results). This level of synthesis corre- lates well with [r] ...
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The Coalescent with Selection on Copy Number Variants
... duplicated copy and interlocus gene conversion between the two copies are ...duplicated copy causes a strong reduction in polymorphism not only in the duplicated copy but also in its flanking regions, ...
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Copy number polymorphisms and anticancer pharmacogenomics
... This study, to our knowledge, is the first comprehen- sive genome-wide study of the effect of CNVs, from the most extensive array-based and sequencing-based sur- veys of these structural variants, on pharmacologic phe- ...
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TaqMan Copy Number Assays. Protocol
... • The TaqMan ® Genotyping Master Mix, containing AmpliTaq Gold ® DNA Polymerase, UP (Ultra Pure) and dNTPs required for the PCR reactions. The real-time PCR reaction occurs in a single tube or well, and it uses universal ...
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