PNA FISH
Rapid identification of bacteria and candida using pna-fish from blood and peritoneal fluid cultures: a retrospective clinical study
... using PNA-FISH for blood cultures compared to traditional culture methods averaged ...the PNA-FISH method- ology is ease of use, in that multiple final identifications may be generated in a ...
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PNA FISH assay uncovers Enterococcus faecium as a facultative intracellular bacterium in co-culture with Acanthamoeba castellanii
... for FISH resulting in a simple yet highly sensitive and specific hybridization assay (PNA FISH) suited for rapid and accurate identification of ...slide. PNA probe is added and hybridizes to ...
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Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Mycobacterium Cultures
... TB PNA FISH is a new fluorescence in situ hybridization (FISH) method using peptide nucleic acid (PNA) probes for differentiation between species of the Mycobacterium tuberculosis complex ...
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Distribution, Organization, and Ecology of Bacteria in Chronic Wounds
... Determination of distribution and organization of bacteria in wounds. To investigate if bacteria were present, and if so, how they were organized spatially and structurally, PNA FISH was performed on fixed ...
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Rapid Identification of Staphylococcus aureus Directly from Blood Cultures by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes
... aureus PNA FISH assay. Initially, the S. aureus PNA FISH assay method was optimized for perfor- mance on various standard blood culture media by standard smear preparation ...published ...
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A Study on Invasive Fungal Infections among Immunocompromised Patients in a Tertiary Care Hospital.
... ABBREVIATIONS AIDS AST BSI CLSI CMV CT CXR DD DM DMSO E TEST ELISA GMS H&E HCV HPE HIV HSV IFI KOH LAT M M VM MHA MIC MOPS MRI NCA PAS PCR PDA PNA FISH Acquired immune deficiency syndrom[r] ...
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Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes for Rapid Identification of Candida albicans Directly from Blood Culture Bottles
... Reference strains and clinical isolates. Thirteen C. albicans reference strains and nine other reference strains representing phylogenetically related Candida species and Lodderomyces elongisporus, mainly within the C. ...
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Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISH
... Background: Mutations of the BCR-ABL1 fusion gene represent a well established cause of resistance to tyrosine kinase inhibitors. Among the different mutations identified T315I is of particular concern since it is not ...
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Design and Evaluation of Peptide Nucleic Acid Probes for Specific Identification of Candida albicans
... these PNA probes was compared quantitatively against that of DNA probes or DNA probe/helper combinations directed against the same target ...the PNA probes were higher than those of the DNA ...the ...
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Comparison of the Staphylococcus QuickFISH BC Test with the Tube Coagulase Test Performed on Positive Blood Cultures for Evaluation and Application in a Clinical Routine Setting
... Many studies demonstrate that delayed proper therapy in bloodstream infections caused by Staphylococcus aureus increases the mor- tality rate, emphasizing the need to shorten the turnaround time for positive blood ...
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Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens
... Design of the PNA oligonucleotide probe. To identify poten- tially useful oligonucleotides, the freely available Primrose program was used (http://www.cf.ac.uk/biosi/research/biosoft /Primrose/index.html) together ...
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Direct DNA and PNA probe binding to telomeric regions without classical in situ hybridization
... telomere PNA and DNA probes were able to bind to the telomeric regions without the use of either heat or formamide, while the centromere PNA probe still require ...of PNA FISH probes [6,7]. ...
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Electrochemical Characterization of PNA Oligonucleotide of Neuraminidase Gene
... of PNA by AdT DPV method (filled dot: relative peak height or peak height, non-filled dot: ...of PNA (red line) and Brdicka electrolyte itself (green ...of PNA 1 ...of PNA 1 µg/ml , ...
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Voltammetric Determination of Different Antioxidants in Petroleum Products by Working Gold Electrode
... method was sufficiently sensitive with concomitant satisfactory differentiation between the two types of antioxidants. At the same time, the presence of organic solvents ensured perfect solubility of the analyzed ...
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Strut-and-Tie Modelling of R.C. Continuous Deep Beams
... P/ Astr1 51 From 49, 50 and 51, the following expression can be derived for the ultimate force PnA: PnA= 1/[4C sin2θs / ftA Ac + A – C cosθs / fcu Astr1] 52 Where ftA is the maximum tens[r] ...
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Peptide Nucleic Acid (PNA) Antisense Effects in Escherichia coli
... A general problem with the development of antisense agents is poor cellular uptake. The outer membrane of gram negative bacteria can provide impressive resistance against a wide variety of compounds. However, there are ...
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Purification of Nucleic Acids by Hybridization to Affinity Tagged PNA Probes
... tagged PNA capture probes offers an efficient means for the purification of nucleic acids by ...forming PNA and requires no sequence information on the ...
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Recombinant Triplex formed by PNA-TFO: A Molecular Dynamics Simulation Study
... To calculate the binding free energy of DNA-DNA duplex; DNA:DNA duplex is treated as complex, the second strand is taken as ligand, the first strand is treated as receptor. For DNA:PNA duplex; DNA:PNA duplex is treated ...
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Role of inland fishery and aquaculture for food and nutrition security in Nepal
... in fish consumption has not been uniform and adequate across ...to fish diet, but taken as the sign of lack of animal ori- gin food and iodine deficiency in human ...dried fish was possible to high ...
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Fisheries: products and fleet 1976-1977. 1979
... Part A : Supply balance sheets Total fish Fish for consumption total Fresh fish for consumption Processed fish total Salted, dried or smoked fish Fish preparations and conserves Deepfro[r] ...
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