Point mutant
Acceleration of bone-defect repair by using A-W MGC loaded with BMP2 and triple point-mutant HIF1α-expressing BMSCs
... triple point- mutant HIF1 α and BMP2 genes promotes the formation of blood vessel network and bone regeneration; how- ever, the interaction of these exogenous genes with the endogenous angiogenic and ...
10
Characterization of the R572T Point Mutant of a Putative Cleavage Site in Human Foamy Virus Env
... R572T mutant proviral ...R572T mutant clones were ob- served when cleaved Env proteins were provided in trans by cotransfection of pCE-K or pCE-S, which expressed KKK or SSS env, respectively, under the ...
6
A completely transformation-defective point mutant of polyomavirus middle T antigen which retains full associated phosphatidylinositol kinase activity.
... Since the single base pair mutation at amino acid 248 resulted in an MTAg that was completely transformation defective despite possessing wild-type levels of kinase activities, this sugg[r] ...
8
The CD225 Domain of IFITM3 Is Required for both IFITM Protein Association and Inhibition of Influenza A Virus and Dengue Virus Replication
... As noted, IFITM2 and IFITM3 reside in late endosomes and lyso- somes, while IFITM1 localizes more to the early endosomes and the cell surface (2, 12). IFITM3 is 59% identical to IFITM1, with the major difference residing ...
16
The Mdm2 Ubiquitin Ligase Enhances Transcriptional Activity of Human Papillomavirus E2
... a point mutant of Mdm2 that no longer has ubiquitin ligase enzymatic activity (Mdm2C462A; kindly provided by Claudio Schneider) and including proteasome in- hibition as an additional ...Mdm2C462A ...
6
Degradation of the Retinoblastoma Tumor Suppressor by the Human Papillomavirus Type 16 E7 Oncoprotein Is Important for Functional Inactivation and Is Separable from Proteasomal Degradation of E7
... or mutant forms of pRB, the E7-binding-defective pRB double point mutant (pRB Y756F/N757A), wild-type HPV-16 E7 (16E7), and a pRB-binding-deficient HPV-16 E7 mutant (16E7 ⌬D21-C24) or control ...
9
The nsp3 Macrodomain Promotes Virulence in Mice with Coronavirus-Induced Encephalitis
... catalytic point mutant virus (N1347A) resulted in reductions in lethality, weight loss, viral titers, proinflammatory cytokine and chemokine expression, and immune cell infiltra- tion in the brain compared ...
14
Simian Virus 40 Vp1 DNA-Binding Domain Is Functionally Separable from the Overlapping Nuclear Localization Signal and Is Required for Effective Virion Formation and Full Viability
... using mutant NO-SV40 viral ge- nomes with the application of the NLS phenotypic comple- mentation we have previously established ...their mutant Vp1s were unable to enter the nucleus de- spite the presence ...
9
Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses.
... this point mutant and deletion mutants of CAEV as well as a deletion mutant of visna virus, we demonstrated that dUTPase- deficient viruses replicate similarly to wild-type viruses in dividing cells ...
5
Induction of susceptibility to tumor necrosis factor by E1A is dependent on binding to either p300 or p105-Rb and induction of DNA synthesis.
... Previous work with acute virus infection of NIH 3T3 cells showed that residues within CR1 are responsible for induction of TNF sensitivity (19). Previous studies have shown that this region of E1A binds both p300 and ...
10
P53-R273H mutation enhances colorectal cancer stemness through regulating specific lncRNAs
... endogenous point mutant cell lines were derived from original parental cell line by continuous exposure to stepwise increasing concentrations of oxaliplatin (Bimake, ...
16
dUTPase-minus caprine arthritis-encephalitis virus is attenuated for pathogenesis and accumulates G-to-A substitutions.
... single point mutants, and the WT ...the mutant viruses showed less-extensive lesions, usually confined to the inoculated ...deletion mutant caused mild mononuclear cell infiltration ...The ...
9
Mutational analysis of a C-dependent late promoter of bacteriophage Mu.
... vector sequences adjacent to a deletion endpoint may influence promoter activity, more precise definition of the critical bases will require point mutant analysis as [r] ...
11
Analysis of the stability and affinity of BlaR-CTD protein to β-lactam antibiotics based on docking and mutagenesis studies
... The activity assay was based on directly competitive inhibition of binding of horseradish peroxidase-labeled ampicillin (HRP-AMP) to the immobilized BlaR-CTD or its mutants by β-lactams. HRP-AMP was prepared according to ...
17
The Small RNA ncS35 Regulates Growth in Burkholderia cenocepacia J2315
... deletion mutant, ΔncS35, was ...deletion mutant showed increased aggrega- tion, higher metabolic activity, a higher growth rate, and an increased susceptibility to ...
16
Role of Cryptococcus neoformans Rho1 GTPases in the PKC1 Signaling Pathway in Response to Thermal Stress
... utilizing point mutants and deletion strains subjected to thermal stress and monitoring phosphorylation of the downstream kinase in the cell integrity pathway, Mpk1, as an output for activation of the PKC1 cell ...
14
Automatic test-data generation for testing simulink models
... each mutant from the original program by the program outputs. If a mutant can be distinguished from the original program by at least one of the test cases in the test set, we say the mutant is ...the ...
20
Interaction between the Negative Regulator of Splicing Element and a 3′ Splice Site: Requirement for U1 Small Nuclear Ribonucleoprotein and the 3′ Splice Site Branch Point/Pyrimidine Tract
... some assembly by native gel analysis revealed the ATP-depen- dent prespliceosomal A complex and mature spliceosomal B complex (15, 16). Subsequently, it was shown that the earliest detectable complex in assembly is the ...
7
PubMedCentral-PMC5530654.pdf
... The hypersensitive ARR10 lines also exhibit an enhanced tran- scriptional response to cytokinin (Fig. 1 G and H). The cytokinin- mediated induction of ARR7, ARR15, and CKX5 and the repression of HKT1 were examined (8, ...
10
Molecular Mechanisms of a Retinal Cyclic Nucleotide-Gated Channel
... voltage-gated mutant channels using mainly the E363Q mutant as an ...This mutant not only is practically fully voltage gated but also expresses sufficient current for a relatively more detailed ...
165