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Polar body

Mechanism of the chromosome-induced polar body extrusion in mouse eggs

Mechanism of the chromosome-induced polar body extrusion in mouse eggs

... The chromosome-induced spindle and cortical actomyo- sin cap play distinct roles in polar body extrusion (Fig- ure 5). Our results show that the cortical cap undergoes protrusion after anaphase onset which ...

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Neonatal Outcome of Preimplantation Genetic Diagnosis by Polar Body Removal: The First 109 Infants

Neonatal Outcome of Preimplantation Genetic Diagnosis by Polar Body Removal: The First 109 Infants

... There were 91 infants born after PGD for chromo- somal disorders and 18 infants born after PGD for Mendelian disorders, including cystic fibrosis (11 in- fants), sickle cell disease (1 infant), long chain acyl CoA ...

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Kit ligand promotes first polar body extrusion of mouse preovulatory oocytes

Kit ligand promotes first polar body extrusion of mouse preovulatory oocytes

... first polar body extrusion by 55% ...first polar body extrusion ...first polar body extrusion, but not GVBD, it is apparent that sequential steps of nuclear maturation of the ...

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MKlp2 inhibitior paprotrain affects polar body extrusion during mouse oocyte maturation

MKlp2 inhibitior paprotrain affects polar body extrusion during mouse oocyte maturation

... To confirm our hypothesis, we investigated the role of MKlp2 in oocyte meiotic maturation using paprotrain, a cell-permeable acrylonitrile compound that inhibits MKlp2. We first examined the localization of MKlp2 during ...

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The eggshell is required for meiotic fidelity, polar-body extrusion and polarization of the C. elegansembryo

The eggshell is required for meiotic fidelity, polar-body extrusion and polarization of the C. elegansembryo

... meiosis, polar-body extrusion and polarization, analogous to the role played by the extracellular matrix in supporting focal adhesion in migrating cells or extending axonal growth ...of polar- ...

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Chromatin organization and timing of polar body I extrusion identify developmentally competent mouse oocytes

Chromatin organization and timing of polar body I extrusion identify developmentally competent mouse oocytes

... Altogether, these data indicate a molecular signature of the de- velopmental potential of oocytes with an SN chromatin organization. Nevertheless, the use of an invasive marker like the fluorochrome Hoechst is not, for ...

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GnRH agonist versus GnRH antagonist in assisted reproduction cycles: oocyte morphology

GnRH agonist versus GnRH antagonist in assisted reproduction cycles: oocyte morphology

... intact polar body, a clear zona pellucida, a small perivitelline space, and a transparent and smooth cytoplasm without granules or inclusions ...the polar body, zona pellucida, perivitelline ...

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Cortical granule exocytosis in C  elegans is regulated by cell
cycle components including separase

Cortical granule exocytosis in C elegans is regulated by cell cycle components including separase

... In attempt to observe the rapid process of degranulation, we imaged embryos using swept field confocal (SFC) microscopy (Prairie Technologies, Middleton, WI, USA) to acquire images every 200 milliseconds. Embryos ...

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Maternal Transmission Ratio Distortion at the Mouse Om Locus Results From Meiotic Drive at the Second Meiotic Division

Maternal Transmission Ratio Distortion at the Mouse Om Locus Results From Meiotic Drive at the Second Meiotic Division

... C57BL/6 males. Although significant lethality occurs in this backcross (ⵑ50%), differences in the level of TRD found in recombinant vs. nonrecombinant chromosomes among offspring argue that TRD is due to nonrandom ...

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Analysis of pronuclear zygote configurations in 459 clinical pregnancies obtained with assisted reproductive technique procedures

Analysis of pronuclear zygote configurations in 459 clinical pregnancies obtained with assisted reproductive technique procedures

... the polar body alignment and the zygote configuration in 459 clinical pregnancies obtained by IVF and ICSI in our public clinic in Reggio Emilia, ...

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Reproductive_System.ppt

Reproductive_System.ppt

... secondary oocyte and the first polar body (when the follicle ruptures, ovulation is... Meiosis II : The secondary oocyte is frozen at metaphase II and will only continue the division [r] ...

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Preimplantation genetic diagnosis and the biopsy technique: Important considerations

Preimplantation genetic diagnosis and the biopsy technique: Important considerations

... Preimplantation genetic diagnosis allows to test the genetic status of embryos prior to implanta- tion. In order to obtain genetic material, on which carry out a genetic diagnosis, a procedure named embryo biopsy is ...

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Cell cycle-dependent regulation of structure of endoplasmic reticulum and inositol 1,4,5-trisphosphate-induced Ca2+ release in mouse oocytes and embryos

Cell cycle-dependent regulation of structure of endoplasmic reticulum and inositol 1,4,5-trisphosphate-induced Ca2+ release in mouse oocytes and embryos

... Inhibition of cdk1-Cyclin B Activity Causes Premature Loss of ER Clusters in Unfertilized Eggs The experiments described above show that high cdk1-cy- clin B activity is sufficient to maintain the presence of ER clusters ...

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Original Article Vitrification of mouse oocyte versus slow freezing: evaluation of ultrastructure and developmental potential

Original Article Vitrification of mouse oocyte versus slow freezing: evaluation of ultrastructure and developmental potential

... first polar body were estimated by ...first polar body was in a better grade of vitrification-thawing group as compared to the slow freezing-thawing ...

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Eccentric position of the germinal vesicle and cortical flow during oocyte maturation specify the animal vegetal axis of ascidian embryos

Eccentric position of the germinal vesicle and cortical flow during oocyte maturation specify the animal vegetal axis of ascidian embryos

... In mice, oocyte maturation has been investigated extensively. Our results are reminiscent of studies using mouse oocytes. In mouse oocytes, the GV is located centrally. The MA forms at the center, and after random slow ...

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SYNTHESIS OF POLY (L-LACTIC ACID) BY EHYDROPOLYCONDENSATION, DETERMINATION OF SEQUENCE BY 13C NMR METHOD

SYNTHESIS OF POLY (L-LACTIC ACID) BY EHYDROPOLYCONDENSATION, DETERMINATION OF SEQUENCE BY 13C NMR METHOD

... non polar) has played an important role during polymerization process in non polar solvents, the probability of higher isotactic hexad sequences were predominant over polar ...

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A self-associating protein critical for chromosome attachment, division, and polar organization in caulobacter

A self-associating protein critical for chromosome attachment, division, and polar organization in caulobacter

... (A) Model by which PopZ mediates the attachment of ori at the poles and thereby regulates cell division. Before DNA replication is initiated, the chromosome is tethered at the old pole via an in- teraction between PopZ ...

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Validity of the Polar M430 Among Females of Varying Fitness Levels, Body Fat Percentage, and Reported Physical Activity

Validity of the Polar M430 Among Females of Varying Fitness Levels, Body Fat Percentage, and Reported Physical Activity

... Next, Body composition was measured for each subject using air displacement plethysmography (BodPod; Life Measurement Instruments, Concord, CA) according to the manufacturer’s ...instructions. Body mass ...

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Ethanolic extract of pomegranate (Punica granatum L) peel: acute toxicity tests on zebrafish (Danio rerio) embryos and its toxicity prediction by in silico

Ethanolic extract of pomegranate (Punica granatum L) peel: acute toxicity tests on zebrafish (Danio rerio) embryos and its toxicity prediction by in silico

... more polar compounds than semi-polar and non-polar compounds, although the three classes of compounds are contained in pomegranate ethanol ...

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PROFILING OF BIOACTIVE COMPOUNDS IN WATER CRESS (ENHYDRA FLUCTUANS LOUR) BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

PROFILING OF BIOACTIVE COMPOUNDS IN WATER CRESS (ENHYDRA FLUCTUANS LOUR) BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

... non- polar (n-hexane: Acetone = 3:1) and polar (CHCl3: CH3OH: H2O =40:10:1) solvent systems exhibits more spots on chromatogram than the TLC plate run in medium polar (CHCl3: CH3OH =5:1) solvent ...

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