poly(rC) binding protein 2
Hepatitis C Virus Internal Ribosome Entry Site-Dependent Translation in Saccharomyces cerevisiae Is Independent of Polypyrimidine Tract-Binding Protein, Poly(rC)-Binding Protein 2, and La Protein
... cap- binding protein eIF4E and the 5 ⬘ -cap structure of the ...internal binding of the 40S ribosomal subunit to an internal ribosome entry site (IRES) ...La protein (3), polypyrimidine ...
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Interaction of Poly(rC) Binding Protein 2 with the 5′ Noncoding Region of Hepatitis A Virus RNA and Its Effects on Translation
... for binding of PCBP2 to PV stem-loop IV and that the binding site is located within the 59 third of the HAV ...direct binding to PCBP2 resulted in only weak and nonspecific retarded migration of the ...
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Interactions of Viral Protein 3CD and Poly(rC) Binding Protein with the 5′ Untranslated Region of the Poliovirus Genome
... FIG. 2. Effect of mutations within stem-loop IV on PCBP binding and viral ...(C) Binding affinity of PCBP to wild-type and mutated stem-loop IV ...
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Replication of Poliovirus Requires Binding of the Poly(rC) Binding Protein to the Cloverleaf as Well as to the Adjacent C-Rich Spacer Sequence between the Cloverleaf and the Internal Ribosomal Entry Site
... When only one A residue was changed back in group II, the variants still expressed a lethal phenotype, regardless of the position of the A residue within the cluster (Fig. 2, M4 to M6). In contrast, a single ...
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Distinct Poly(rC) Binding Protein KH Domain Determinants for Poliovirus Translation Initiation and Viral RNA Replication
... RNA binding surface to those coding for ...solvent-exposed protein domains without causing major disruption in the overall pro- tein conformation ...RNA binding as- says with stem-loop I ...(lanes ...
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Poly(rC) Binding Proteins and the 5′ Cloverleaf of Uncapped Poliovirus mRNA Function during De Novo Assembly of Polysomes
... Uncapped mRNAs are typically degraded by 5 ⬘ exonuclease (45); however, PV mRNA, which is naturally uncapped (22, 39, 40), is able to form stable polysomes (Fig. 8). Our experimental results indicate that uncapped PV ...
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Cellular Protein Modification by Poliovirus: the Two Faces of Poly(rC)-Binding Protein
... cellular protein poly(rC)-binding protein (PCBP) was identified as a primary candidate for regulating such a ...functional protein-protein and RNA-protein complexes ...
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Presence of a Poly(A) Binding Protein and Two Proteins with Cell Cycle-Dependent Phosphorylation in Crithidia fasciculata mRNA Cycling Sequence Binding Protein II
... RBP63 protein shows approximately a 10-fold preference in binding the wild-type RNA probe (containing 6⫻ CAUAGAAG) com- pared to mutant probe (containing 6⫻ ...tors, poly(A) competed out the ...
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Functional Characterization of Three Leishmania Poly(A) Binding Protein Homologues with Distinct Binding Properties to RNA and Protein Partners
... either protein was observed in the precipitated complexes, with both supernatant and input samples containing equivalent quantities of the two ...each protein, followed by incubation with Alexa ...
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Interplay between the Poly(A) Tail, Poly(A)-Binding Protein, and Coronavirus Nucleocapsid Protein Regulates Gene Expression of Coronavirus and the Host Cell
... the poly(A) tail and N protein with translation factor eIF4G and coronavirus replicase protein ...the poly(A) tail and N protein in gene regulation, we first determined whether the ...
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RNA-Binding Domain Proteins in Kinetoplastids: a Comparative Analysis
... (Table 2). These were two poly(A) binding proteins; splicing factors U1 70k, U2AF35, and U2AF65; rRNA-pro- cessing protein MRD1; peptidyl-prolyl cis-trans isomerase; hnRNPH; translation factor ...
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Poly(A)-Binding Protein Facilitates Translation of an Uncapped/Nonpolyadenylated Viral RNA by Binding to the 3′ Untranslated Region
... a capsid protein encoded in RNA1 are translated via a 3=CITE- mediated translation mechanism (26, 43, 53). The movement pro- tein that is required for cell-to-cell movement is encoded in RNA2, and translated via ...
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Stimulation of translation by human Unr requires cold shock domains 2 and 4, and correlates with poly(A) binding protein interaction
... Figure 1. Unr stimulates translation in vitro. (a–d) In vitro translation of uncapped non-polyadenylated (a), uncapped polyadenylated (b), capped non-polyadenylated (c) or capped polyadenylated (d) Renilla luciferase ...
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Cleavage of Poly(A)-Binding Protein by Enterovirus Proteases Concurrent with Inhibition of Translation In Vitro
... viral protein expression (data not shown), but it has never been complete by 6 h ...by 2 h ...dant protein, having a half-life greater than 8 h ...
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Poly(A) Polymerase and the Nuclear Poly(A) Binding Protein, PABPN1, Coordinate the Splicing and Degradation of a Subset of Human Pre-mRNAs
... PAP and PABPN1 are important for a subset of endogenous splicing events. A recent study addressed the global impact of PABPN1 depletion on mRNA levels and found that, in most cases, it had little effect (50). However, ...
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Efficient Cleavage of Ribosome-Associated Poly(A)-Binding Protein by Enterovirus 3C Protease
... a binding cleft that binds PAIP-1, PAIP-2 (which function in 5⬘-3⬘ interactions), and eRF3 (which functions in ribosome termination) (41, ...and binding of 60S ribosomal subunits during de novo ...
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Rubella Virus Capsid Protein Interacts with Poly(A)-Binding Protein and Inhibits Translation
... of binding to an RNA ...PABP. Binding of virus proteins to cellular proteins can have a number of effects, including relocalization, blocking their function, or inducing degradation of the cellular ...
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Rotavirus NSP3 Is a Translational Surrogate of the Poly(A) Binding Protein-Poly(A) Complex
... cellular poly(A) mRNA expres- sion, an MA104 cell line constitutively expressing a destabilized renilla luciferase (24) was ...to 2 h after infection, probably due to a transient inhibition of the ...
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Translational Control of the Abundance of Cytoplasmic Poly(A) Binding Protein in Human Cytomegalovirus-Infected Cells
... regulatory protein, it is not absolutely required at high MOIs, similar to IE1 in HCMV- infected cells ...a protein product that directly associates with eIF4G, promotes binding to eIF4E, and facil- ...
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Utilization of Poly(A)-Binding Protein in Monitoring the Formation of mRNP Granules in Toxoplasma gondii
... PABP protein with td- Tomato at the C-terminus, ...treatment, 2 μL of the insert and 1 μL of the vector were mixed at room temperature for 10 minutes followed with addition of 1 μL of 25 mM EDTA and ...
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