polymerase chain reaction techniques
Diagnosing genital tuberculosis in female infertility by clinical, histopathological, culture and polymerase chain reaction techniques.
... positive reaction may be produced in BCG vaccinated persons. As the reaction involves cell mediated immunity, in immuno compromised individuals with human immunodeficiency virus (HIV) infection, uremia, ...
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Reviewing Classical and Molecular Techniques Regarding Profiling of Probiotic Character of Microorganisms
... cultivation techniques or have died during transport and ...(FISH), polymerase chain reaction techniques (PCR amplification followed by cloning and sequencing), Temperature gradient gel ...
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Strategies for large-scale production of commercially important banana varieties of Mizoram, India, using plant tissue culture technique
... The prospective mother plants should be thoroughly evaluated and selected for its supe- rior agronomic characteristics and freedom from diseases. The selected plants should be main- tained in a protective area where soil ...
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Clustering techniques for DNA computing readout method based on real-time polymerase chain reaction
... Adleman proved that this algorithm can be implemented in molecular level. Adleman used a set of 20-mer oligonucleotides, or oligos, to encode each vertex and edge, which is randomly designed in advance. To implement the ...
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Assessment of tubercle bacilli in various organs by staining and polymerase chain reaction technique
... Methods: The study included data 14,472 patients of both prospective (during March 2008 to 2009) and retrospective cases (past one year of the study period). For prospective cases the cytological material for the study ...
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A New Sensitive Method for Detection of Viroids
... diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (RT-PCR) are being used for detection and diagnosis of ...
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Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples
... The hnRT-PCR technique allows a sensitive, specific and fast diagnosis for rabies virus, even when samples are in a decomposed state. Additionally, the amplified products can be used in techniques such as ...
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One-Step RT-qPCR assay for detection and quantification of equine infectious anemia virus in-vitro
... isolation techniques or polymerase chain reaction (PCR) along with mathematical models are useful to determine certain aspects of in-vitro virus infection that are usually associated with ...
9
INHIBITION OF POLYMERASE CHAIN REACTION BY LITHIUM CHLORIDE
... DNA polymerase) complex, and denaturation, annealing and extension of DNA strands are thermally ...related techniques [ 12,13 ] and therefore study of its effect on any process associated with DNA ...
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Mitochondrial uncoupling protein 1 expression in thymocytes
... multiple techniques [4 – 8], including: (i) reverse transcriptase-polymerase chain reaction detection of RNA transcripts for UCP 1 in whole thymus and in isolated thymocytes of rats and mice, ...
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Molecular biology techniques and applications for ocean sensing
... biological techniques is now widespread in the ocean sciences. These techniques target nucleic acids which record the evolutionary history of microbes, and encode for pro- cesses which are active in the ...
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Polymerase Chain Reaction (PCR) Techniques For The Identification Of Uncultivable Oral Microorganisms
... Until today there are still a high percentage of oral microorganisms have not been identified due to inability to isolate using the cultural method. However, identification of uncultivable microorganisms associated with ...
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The polymerase chain reaction in the charaterisation and diagnosis of lymphomas
... -Ô chain genes allow identification of monoclonal populations of T- ...these techniques are tim e consum ing, use radioactive isotopes an d depend on the availability of fresh/frozen tissu e sam ples, and ...
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Molecular Variability of Fusarium verticillioides (Sacc ) in Maize from Three Agro Ecological Zones of Southwest Nigeria
... molecular techniques has been adopted; they are RAPD (Random Amplified Polymorphic DNA), PCR- RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism), AFLP (Amplified ...
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Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases.
... The incidence of liver cancer in China has gradually increased. The rate of liver cancer recurrence is still high despite various procedures and therapies (Lemoine et al., 1997). Research has shown that the 5-year ...
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Sulfur mustard induces expression of metallothionein-1A in human airway epithelial cells
... transcriptase polymerase chain reaction was then performed in a Rotor-Gene RG 3000 (Corbett Research, Mortlake, ...Quantitative polymerase chain reaction criteria comprised ...
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5′ Nuclease PCR Assay To DetectYersinia pestis
... PCR techniques have significantly improved the detection and identification of bacterial pathogens. Because of their high specificity and sensitivity, PCR-based assays can be extremely important when rapid, ...
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Polymerase chain reaction assay for detection of human cytomegalovirus
... Urine specimens from six renal transplant recipients which had been previously characterized as positive for the presence of HCMV by either ELISA, hybridization assay with a biotinlabele[r] ...
5
Detection of Mycoplasma pneumoniae by using the polymerase chain reaction
... B Southern blot showing the specific amplified product obtained by amplification of extract from hamster H5 on day 4 after infection lane 2 and hamster H3 on day 8 after infection lane 3[r] ...
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Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... amplification. The primer concentrations in the reaction mixtures of 25 μl were 600 nM for each forward and reverse primer. A total of 8 μl of DNA extracted from the vitreous fluid sample was used as a PCR ...
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