polymerase chain reactions
Coamplified positive control detects inhibition of polymerase chain reactions
... The coamplified control has a distinct advantage over parallel control amplifications because it identifies random amplification failures, further increasing the confidence in results fr[r] ...
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Optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens
... First, the amplification efficiency and quantification limit of the selected primer pairs were tested using a 10-fold standard dilution series.. The best performing primer pairs were sel[r] ...
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Development of novel multiplex microsatellite polymerase chain reactions to enable high throughput population genetic studies of Schistosoma haematobium
... μl reactions using 2 μl of the eluted DNA and the Type-it Microsatellite PCR Kit (Qiagen) according to the manufacturer’s protocol with the addition of ...mins. Reactions were checked by 3 % agarose gel ...
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Two step polymerase chain reactions and restriction endonuclease analyses detect and differentiate ompA DNA of Chlamydia spp
... Knowledge of the partial MOMP gene DNA sequences of the chlamydial MOMP genotypes listed in Tables 1 and 3 enabled us to further differentiate them as ompA RFLP types by restriction endo[r] ...
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Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions
... Detection of group A rotavirus in serial fecal specimens by RT-PCR: extraction and dilution compared with extraction and CF11 purification RT-PCR results Fecal specimen mo-day collected.[r] ...
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COMPARING THE GENES OF Artemisia annua AND Artemisia ludoviciana WITH RESPECT TO THE ARTEMISININ BIOSYNTHETIC PATHWAY
... transcriptase polymerase chain reactions (RT-PCR) done on RNA extracts obtained from the fresh leaves of Artemisia ludoviciana with the Artemisia annua primers are summarized in table ...
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Development of RAPD, DAMD and ISSR markers for authentication of medicinal plant Cassia auriculata and its adulterant Cassia surattensis
... Abstract — Cassia auriculata is an important traditional medicinal plant commonly used in many Ayurvedic formulations, meant for diabetes, rheumatism, conjunctivitis, infertility and etc. But due to similar morphological ...
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<em>MSX1</em> induces G0/G1 arrest and apoptosis by suppressing Notch signaling and is frequently methylated in cervical cancer
... transcription-polymerase chain reactions were used to investigate the expression levels of MSX1, and methylation-specific polymerase chain reaction (MSP) was performed to investigate ...
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Uropathogenic Escherichia coli in Iran: Serogroup distributions, virulence factors and antimicrobial resistance properties
... Methods: Totally, 123 strains of Escherichia coli isolated from UTIs patients, using bacterial culture method were subjected to polymerase chain reactions for detection of various O- ser[r] ...
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Construction of a DNA probe and detection of Actinobacillus pleuropneumoniae by using polymerase chain reaction
... From the nucleotide sequence of the putative genomic probe, three primers were synthesized for use in polymerase chain reactions PCRs, with 31 strains tested by using purified and crude [r] ...
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On the Chain Length and Rate of Ozone Depletion in the Main Stratospheric Cycles
... (see [11,14]). In this connection it should be underlined that as it follows from Chapman’s mechanism, ozone can be destructed only through depletion of the odd oxygen which requires at least two reaction of chain ...
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Synthesis of bifunctional monomers by the palladium catalyzed carbonylation of cardanol and its derivatives
... compounds with double bonds located deep in the chain. For complete utilization of these oils to make polymers, the internal double bonds must be isomerized to the least thermodynamically favored terminal position ...
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INHIBITION OF POLYMERASE CHAIN REACTION BY LITHIUM CHLORIDE
... (http://www.promega.com/paguide/chap1.htm). In the absence of adequate free magnesium ions, Taq DNA polymerase is inactive. Thus with the increased concentration of Mg 2+ ions (in the form of MgCl 2 ) the ...
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Polymerase chain reaction on a viral nanoparticle
... 3 An alternative approach to the construction of materials from biological components has been to exploit an existing fibrous chassis that has been evolved within nature, which concentrates on functionalization of a ...
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Polymerase chain reaction: trenches to benches
... First described in 1985, this ingenious method uses repeated cycles of oligonucleotide-directed DNA synthesis to carry out in vitro replication of target nucleic acid sequences, forming [r] ...
5
DNA Based Steganography
... primers would be able to extract the message, primers would be able to extract the message, using the polymerase chain reaction (PCR) to using the polymerase ch[r] ...
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Signal Transduction with Hybridization Chain Reactions
... Four contributions to the field of nucleic acid engineering are presented: (1) the con- struction of a single nucleotide polymorphism (SNP) detector based on the mechanism of hybridizati[r] ...
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Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis
... for polymerase elongation by functioning as primer upon binding to the target se- ...The reactions performed to deter- mine the sensitivity of the LF-RPA were constantly shaken during the ...
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5′ Nuclease PCR Assay To DetectYersinia pestis
... less than 60 s. The ABI 7700 employs a computer algorithm to calculate a value termed DRn as follows: DRn 5 Rn1 2 Rn2, where Rn1 is the emission intensity of the reporter divided by the emission intensity of the quencher ...
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Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR
... PCR: polymerase chain reaction; RT-PCR: reverse tran- scriptase polymerase chain reaction; CTCs: circulating tumour cells; PBMNCs: peripheral blood mononuclear cells; IB RT-PCR: immunobead ...
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