POLYMERASE COMPLEX
Comprehensive Proteomic Analysis of Influenza Virus Polymerase Complex Reveals a Novel Association with Mitochondrial Proteins and RNA Polymerase Accessory Factors
... virus polymerase complex or its ...[VN/1203]) polymerase (3P) com- plex and its subunit components (PA and PA-PB1) from human lung epithelial cells (A549 cell line) infected with adenovirus (Ad) ...
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Association of the polioviral RNA polymerase complex with phospholipid membranes.
... Comparison of both the host- and viral-specific polypeptides that spontaneously associated with the liposomes, found in the polymerase-rich membrane fraction of the cell and in the purif[r] ...
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Influenza virus polymerase basic protein 1 interacts with influenza virus polymerase basic protein 2 at multiple sites.
... its polymerase activity were studied by reconstitution of the active polymerase complex in ...RNP complex (6, 16, ...for polymerase activ- ...for polymerase activity (12, ...the ...
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Association of the Influenza Virus RNA Polymerase Subunit PB2 with the Host Chaperonin CCT
... RNA polymerase of influenza A virus is a host range determinant and virulence ...RNA polymerase has been implicated as a crucial factor that affects cell tropism as well as virulence in animal ...
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Introduction of Virulence Markers in PB2 of Pandemic Swine-Origin Influenza Virus Does Not Result in Enhanced Virulence or Transmission
... Minigenome assays. Minigenome assays were used to study the effects of three amino acid substitutions in the PB2 gene, E627K, E677G, and D701N, on the activity of the influenza virus polymerase complex. ...
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Possible in vitro repair of viral RNA by ligase-like enzyme(s) in poliovirus-infected cells.
... The quantitative differences of AU activity One each was assayed with the complete incubation between different viral polymerase complex mixture, and the other was incubated with the pre[r] ...
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Releasing the Genomic RNA Sequestered in the Mumps Virus Nucleocapsid
... RNA polymerase (vRdRp) must be able to gain access to the se- questered genomic RNA in order to use it as the ...viral polymerase complex consists of the large protein (L) and the phosphoprotein ...
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Identification of Influenza A Virus PB2 Residues Involved in Enhanced Polymerase Activity and Virus Growth in Mammalian Cells at Low Temperatures
... increased polymerase activity in mammalian cells at low temperature ...lowest polymerase activity among the five chimeras ...hanced polymerase activity at low ...pH1N1 polymerase activ- ity in ...
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Molecular Determinants of Adaptation of Highly Pathogenic Avian Influenza H7N7 Viruses to Efficient Replication in the Human Host
... basic polymerase 2 (PB2), acidic polymerase (PA), HA, neuraminidase (NA), and nonstructural protein 1 (NS1) compared to the CC virus (20) (Table ...the polymerase complex proteins, plays an ...
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Influenza A Virus Polymerase Is an Integral Component of the CPSF30-NS1A Protein Complex in Infected Cells
... viral polymerase complex in infected ...the complex of the Ud effector domain with the F2F3 domain of CPSF30 ...This complex is a tetramer that con- tains two NS1A effector domains and two ...
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Structural and Functional Characterization of an Influenza Virus RNA Polymerase-Genomic RNA Complex
... of polymerase-RNA complexes requires the polymerization activity of viral ...generate polymerase-vRNA complexes using a polymerase with a lethal mutation in PB1 led to complete ...merized ...
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Adaptation of Borna Disease Virus to New Host Species Attributed to Altered Regulation of Viral Polymerase Activity
... L polymerase (L1116R and N1398D) and in the polymerase cofactor P ...viral polymerase activity rather than adaptation to a mouse-specific ...of polymerase activity by the viral X ...the ...
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Configuration of Viral Ribonucleoprotein Complexes within the Influenza A Virion
... viral polymerase complex is thought to associate ...few polymerase complexes are located at the end of the rod-like RNP (1, 3) and that therefore the polymerase complexes are located at the ...
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Epstein-Barr virus nuclear protein 2 (EBNA2) binds to a component of the human SNF-SWI complex, hSNF5/Ini1.
... RNA polymerase II transcription complex, including TFIIB, TFIIH (62- and 80-kDa subunits), TAF40, and a P100 protein associated with TFIIE ...hSNF-SWI complex that may be a part of the RNA ...
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Poliovirus Polymerase Residue 5 Plays a Critical Role in Elongation Complex Stability
... the complex may involve hydrophobic interac- tions that would be favored at the higher salt ...elongation complex stability also reduced polymerase processivity during replication of longer RNA tem- ...
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Host-Specific Replication of BK Virus DNA in Mouse Cell Extracts Is Independently Controlled by DNA Polymerase α-Primase and Inhibitory Activities
... Interestingly, in mouse extracts human Pol-prim and BKV TAg cooperated to replicate DNA containing P-B-B and P-B-P chimeric origins, both of which have a mouse polyomaviral early region, whereas the wild-type origin, ...
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A Complex of the Bacteriophage T7 Primase-Helicase and DNA Polymerase Directs Primer Utilization
... DNA polymerase, provided the intact primase-helicase protein is added during primer extension ...DNA polymerase suggested that several subunits of the hex- americ primase-helicase might cooperate to deliver ...
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Intergenic Transcriptional Interference Is Blocked by RNA Polymerase III Transcription Factor TFIIIB in Saccharomyces cerevisiae
... these transcripts at low levels (Wolin et al. 2012), it has become clear that the act of intergenic transcription can have significant effects on neighboring genes. Due to such observations, one must consider how mutation ...
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Structural basis for H-NS-mediated trapping of RNA polymerase in the open initiation complex at the rrnB P1
... RNA polymerase in the open initiation complex at the rrnB P1 promoter, we constructed a linear DNA fragment of around 1200 bp containing the transcription start site ( ⫹ 1) close to its center (see ...
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Improved Production of Gutted Adenovirus in Cells Expressing Adenovirus Preterminal Protein and DNA Polymerase
... Ad polymerase expression plas- ...DNA polymerase bind to each other and function as a complex in the initiation of Ad DNA replication (22, ...both polymerase and preterminal protein in the ...
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