Polypyrimidine tract-binding protein 1
Polypyrimidine Tract-Binding Protein Binds to the Leader RNA of Mouse Hepatitis Virus and Serves as a Regulator of Viral Transcription
... (lane 1, 100%) and 112(1) (lane 2, 70%) RNAs were comparable but that the amount of its binding to RNA 56(1) (lane 3) was significantly lower ...the binding of ...PTB binding to ...
6
Specific interaction of polypyrimidine tract-binding protein with the extreme 3'-terminal structure of the hepatitis C virus genome, the 3'X.
... Figure 1 shows maps of the 3 9 -end RNA constructs of HCV (positive ...with 1 ng of pUC 9106-3 9 X as the ...(Table 1), contained an intact T7 promoter sequence; therefore, resulting DNA fragments ...
7
Interaction of HIV 1 integrase with polypyrimidine tract binding protein and associated splicing factor (PSF) and its impact on HIV 1 replication
... rigid-body protein–protein docking algorithm, ZDOCK [61] was used to perform unbound ...the Protein Data Bank. The full length (1–288 amino acids) structure of HIV-1 Inte- grase was ...
18
Polypyrimidine tract-binding protein and heterogeneous nuclear ribonucleoprotein A1 bind to human T-cell leukemia virus type 2 RNA regulatory elements.
... I binding was observed at high levels ...little binding of hnRNP A1 was observed ...to binding by hnRNP I but not by hnRNP ...and binding by both hnRNP I ...the binding by hnRNP I ...
7
Original Article Inhibition of hepatitis B virus in HepG2.2.15 by indirubin through suppression of a polypyrimidine tract-binding protein
... Chronic hepatitis B virus (HBV) infection contin- ues to be a major health burden worldwide [1]. Current first-line drugs for chronic hepatitis B include pegylated interferon alpha2a (PEG-IFN) or nucleotide analog ...
8
Polypyrimidine Tract Binding Protein Induces Human Papillomavirus Type 16 Late Gene Expression by Interfering with Splicing Inhibitory Elements at the Major Late 5′ Splice Site, SD3632
... To test if sequences adjacent to SD3632, rather than SD3632 itself, were required for the PTB response, we deleted SD3632 and the sequence upstream of SD3632 in pBSpliceM, resulting in pMT1 (Fig. 8A). As expected, this ...
14
The Polypyrimidine Tract-Binding Protein Affects Coronavirus RNA Accumulation Levels and Relocalizes Viral RNAs to Novel Cytoplasmic Domains Different from Replication-Transcription Sites
... the binding of PTB to viral RNA sequences involved in transcrip- tion, such as TRS-L and several TRS-Bs, has been ...by 1 mM sodium arsenite for 60 min, (ii) PKR activation by 4 g poly(I:C) for 6 h, or ...
14
Translation of Polioviral mRNA Is Inhibited by Cleavage of Polypyrimidine Tract-Binding Proteins Executed by Polioviral 3Cpro
... containing 1% bovine serum albumin [BSA]) for 30 min at room temperature and then incubated with anti-PTB antibody for 1 h at room ...for 1 h at room ...fluorescence protein (EGFP) and red ...
14
A 57-Nucleotide Upstream Early Polyadenylation Element in Human Papillomavirus Type 16 Interacts with hFip1, CstF-64, hnRNP C1/C2, and Polypyrimidine Tract Binding Protein
... Lane 1, primers A and B; lane 2, primers A and C; lane 3, primers A and ...(Table 1). The location of the RT-PCR primers is shown in panel A. The 1-kb DNA ladder (Invitrogen) was used as a molecular ...
19
Hepatitis C Virus Internal Ribosome Entry Site-Dependent Translation in Saccharomyces cerevisiae Is Independent of Polypyrimidine Tract-Binding Protein, Poly(rC)-Binding Protein 2, and La Protein
... cap- binding protein eIF4E and the 5 ⬘ -cap structure of the ...internal binding of the 40S ribosomal subunit to an internal ribosome entry site (IRES) ...La protein (3), polypyrimidine ...
12
Hypoxia May Increase Rat Insulin mRNA Levels by Promoting Binding of the Polypyrimidine Tract-binding Protein (PTB) to the Pyrimidine-rich Insulin mRNA 3′-Untranslated Region
... Given the similarities between nitric oxide and oxygen (they both bind to ferrous atoms in heme proteins), it is possible that nitric oxide could either mimic or inhibit hypoxia-induced signals. Nitric ox- ide has in ...
10
Polypyrimidine Tract-Binding Protein Binds to the Complementary Strand of the Mouse Hepatitis Virus 3′ Untranslated Region, Thereby Altering RNA Conformation
... study protein-RNA interaction at the previously identified cis-acting signal for transcription in the 3⬘-UTR of MHV RNA, a UV-cross-linking assay was first performed to detect the protein species that can ...
7
Role of Polypyrimidine Tract Binding Protein in the Function of the Hepatitis B Virus Posttranscriptional Regulatory Element
... PTB binding in PRE function. The binding of PTB to fragment III does not necessarily reflect a role for PTB in PRE ...PTB binding, we performed two sets of ...PTB binding on PRE func- tion by ...
8
Attenuating mutations in the poliovirus 5' untranslated region alter its interaction with polypyrimidine tract-binding protein.
... for 1 h in PBS containing 3% (wt/vol) bovine serum albumin and then washed three times in ...diluted 1:150 or a mono- clonal anti-La antiserum (provided by ...diluted 1:100 in incubation buffer ...
8
Functional involvement of polypyrimidine tract-binding protein in translation initiation complexes with the internal ribosome entry site of foot-and-mouth disease virus.
... The poly(U)-Sepharose-treated RRL was then used to an- alyze the effect of added purified recombinant PTB on trans- lation. The dicistronic reporter mRNA (Fig. 6B) contains the CAT gene monitoring 5 9 -dependent ...
10
HuR Displaces Polypyrimidine Tract Binding Protein To Facilitate La Binding to the 3′ Untranslated Region and Enhances Hepatitis C Virus Replication
... HCV-NS3 protein levels were also reduced considerably, indicating that there is a decrease in viral protein ...NS3 protein levels were observed upon FIG 1 Effect of partial silencing of ...NS3 ...
17
The Polypyrimidine Tract Binding Protein Is Required for Efficient Picornavirus Gene Expression and Propagation
... PTB has been implicated in many and diverse RNA trans- actions (50, 51). In the nucleus, PTB is required for silencing alternative exons in FGFR2 and fibronectin and within its own transcripts and is likely involved in ...
8
Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function
... the binding of GAPDH to the HAV IRES, we constructed a series of plasmids containing dicistronic transcriptional units under control of the CMV/T7 composite promoter, as depicted in ...the protein within ...
10
Interaction of polypyrimidine tract-binding protein with the 5' noncoding region of the hepatitis C virus RNA genome and its functional requirement in internal initiation of translation.
... of protein concentration ...similar binding conditions, GST alone was not cross-linked to the probe (lanes 5 and ...fusion protein. The binding specificity of PTB to 5 9 NCR was confirmed by a ...
9
Functional Analysis of RNA Structures Present at the 3′ Extremity of the Murine Norovirus Genome: the Variable Polypyrimidine Tract Plays a Role in Viral Virulence
... single-stranded polypyrimidine tract [p(Y)] of variable length between MNV isolates, within the terminal stem-loop ...pyrimidine binding proteins PTB and PCBP bound the 3 ⴕ -untranslated region via ...
13