Polypyrimidine tract binding protein (PTB)
Polypyrimidine Tract-Binding Protein Binds to the Complementary Strand of the Mouse Hepatitis Virus 3′ Untranslated Region, Thereby Altering RNA Conformation
... major protein species, p55, was identified as the polypyrimidine tract-binding protein (PTB, also known as heterogeneous nuclear RNP I) by immunoprecipitation of the ...
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Specific interaction of polypyrimidine tract-binding protein with the extreme 3'-terminal structure of the hepatitis C virus genome, the 3'X.
... 57-kDa protein (p57), which had higher affinities to RNA probes, recognized a 26-nt sequence including the 5 * -terminal 19 nt of the 3 * X and 7 flanking nt, designated the transitional ...consensus ...
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Expression analysis of the polypyrimidine tract binding protein (PTBP1) and its paralogs PTBP2 and PTBP3 during Xenopus tropicalis embryogenesis
... Fig. 3. Spatial expression profiles of polypyrimidine tract binding protein (PTB) genes during early Xenopus development. Whole-mount in situ hybridization was performed to study the ...
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Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function
... and polypyrimidine tract binding protein (PTB) bind to overlapping sites in stem-loop IIIa of the internal ribosome entry site (IRES) of Hepatitis A virus (HAV), a ...the binding ...
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Original Article Inhibition of hepatitis B virus in HepG2.2.15 by indirubin through suppression of a polypyrimidine tract-binding protein
... Abstract: Chronic hepatitis B virus (HBV) infection continues to be a public health burden worldwide. With the current treatment strategy, a limited number of patients achieve “functional cure”. There is a need for novel ...
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Polypyrimidine Tract-Binding Protein Binds to the Leader RNA of Mouse Hepatitis Virus and Serves as a Regulator of Viral Transcription
... cellular protein, previously described as p55, binds specifically to the plus strand of the mouse hepatitis virus (MHV) leader ...this protein and determined by partial peptide sequencing that it is ...
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Hepatitis C Virus Internal Ribosome Entry Site-Dependent Translation in Saccharomyces cerevisiae Is Independent of Polypyrimidine Tract-Binding Protein, Poly(rC)-Binding Protein 2, and La Protein
... cap- binding protein eIF4E and the 5 ⬘ -cap structure of the ...internal binding of the 40S ribosomal subunit to an internal ribosome entry site (IRES) ...La protein (3), polypyrimidine ...
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Attenuating mutations in the poliovirus 5' untranslated region alter its interaction with polypyrimidine tract-binding protein.
... Mutations in the 5 * untranslated regions (5 * -UTRs) of all three serotypes of the Sabin vaccine strains are known to be major determinants of the attenuation phenotype. To further understand the functional basis of the ...
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A 57-Nucleotide Upstream Early Polyadenylation Element in Human Papillomavirus Type 16 Interacts with hFip1, CstF-64, hnRNP C1/C2, and Polypyrimidine Tract Binding Protein
... hFip1 protein, which has been shown to enhance polyadenylation through U-rich upstream elements, interacted specifically with the HPV-16 upstream ...and polypyrimidine tract binding ...
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Polypyrimidine tract-binding protein and heterogeneous nuclear ribonucleoprotein A1 bind to human T-cell leukemia virus type 2 RNA regulatory elements.
... by binding of specific cellular ...is polypyrimidine tract-binding protein or hnRNP ...A1 binding site in U5 RNA and demonstrated that p40CRS (hnRNP A1) binding to that ...
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Role of Polypyrimidine Tract Binding Protein in the Function of the Hepatitis B Virus Posttranscriptional Regulatory Element
... cognate binding sites (37). Therefore, PTB can potentially increase the export of mRNA by an indirect mech- anism, ...hand, PTB also shuttles rapidly between the nucleus and the cyto- plasm by virtue ...
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Hypoxia May Increase Rat Insulin mRNA Levels by Promoting Binding of the Polypyrimidine Tract-binding Protein (PTB) to the Pyrimidine-rich Insulin mRNA 3′-Untranslated Region
... than binding heme ...on PTB- ins–PRS binding by the nitric oxide donor DETA ...increased PTB expression in rat islets exposed for 24 hr to nitric oxide–inducing cytokines or chemically derived ...
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The Polypyrimidine Tract-Binding Protein Affects Coronavirus RNA Accumulation Levels and Relocalizes Viral RNAs to Novel Cytoplasmic Domains Different from Replication-Transcription Sites
... The binding of PTB to the 5 ⬘ end of the viral genome was shown ...report, PTB interaction with TGEV TRSs has been shown by RNA affinity chromatography and mass spectrometry anal- ...of PTB on ...
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Polypyrimidine Tract Binding Protein Induces Human Papillomavirus Type 16 Late Gene Expression by Interfering with Splicing Inhibitory Elements at the Major Late 5′ Splice Site, SD3632
... the PTB response, we deleted SD3632 and the sequence upstream of SD3632 in pBSpliceM, resulting in pMT1 ...a PTB-expressing ...a PTB-expressing ...
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Interaction of polypyrimidine tract-binding protein with the 5' noncoding region of the hepatitis C virus RNA genome and its functional requirement in internal initiation of translation.
... the binding of PTB to the 5 9 NCR of the ...that PTB, which binds picornavirus 5 9 NCRs at multiple sites (18), also binds the HCV 5 9 NCR and is functionally required for internal initiation of ...
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The Polypyrimidine Tract Binding Protein Is Required for Efficient Picornavirus Gene Expression and Propagation
... of PTB are poor hosts for both EMCV and PV propagation, indicating that PTB is an essen- tial host factor for efficient growth of ...cell protein shutoff by PTB depletion points towards a role ...
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HuR Displaces Polypyrimidine Tract Binding Protein To Facilitate La Binding to the 3′ Untranslated Region and Enhances Hepatitis C Virus Replication
... or PTB decreases the HCV IRES-driven firefly luciferase activity, indicat- ing that all three proteins are essential either for IRES-mediated translation or the replication of the SGR-JFH1/Luc bicistronic RNA ...
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Functional involvement of polypyrimidine tract-binding protein in translation initiation complexes with the internal ribosome entry site of foot-and-mouth disease virus.
... recombinant PTB on trans- ...of PTB caused a stimulation of luciferase expression (lane 3), and increasing amounts of added PTB restored the efficiency of translation to approximately the orig- inal ...
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Interaction of HIV 1 integrase with polypyrimidine tract binding protein and associated splicing factor (PSF) and its impact on HIV 1 replication
... interacting protein, polypyrimidine tract bind- ing protein and associated splicing factor (PSF) through pull down assay and co immunoprecipitation of IN with mammalian cell ...acid ...
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Determination of the secondary structure of and cellular protein binding to the 3'-untranslated region of the hepatitis C virus RNA genome.
... cellular protein (p58) was detected. Its binding site was mapped to the stem-loops 2 and 3, which are the most conserved region of the 3 * ...p58 binding. Mutations that disrupted stem structures ...
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