Primary astrocytes
Sex differences in the inflammatory response of primary astrocytes to lipopolysaccharide
... of astrocytes to LPS. Our results indicate that cortical primary astrocytes derived from male and female mice pups show different expression of inflammatory markers in response to ...female ...
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Andrographolide attenuates LPS-stimulated up-regulation of C-C and C-X-C motif chemokines in rodent cortex and primary astrocytes
... regulating cortical chemokines from the C-C and C-X-C subfamilies in a peripherally administered LPS model. In separate experiments, we showed that andrographolide also attenuated LPS-induced gene up-regulation of a ...
11
Multiple Oxygen Tension Environments Reveal Diverse Patterns of Transcriptional Regulation in Primary Astrocytes
... ]. Sirt2 has also been recently implicated in oxygen tension-related homeostasis, through a regulatory control of the 14-3-3 scaffolding protein and the apoptosis-related factor Bad [70]. In addition, we identified and ...
20
Human Immunodeficiency Virus Type 1 Induces Expression of Complement Factors in Human Astrocytes
... and primary astrocytes are respon- sive toward ...in astrocytes is a general mechanism or restricted to the cell line U373, various astrocytic cell lines and a prep- aration of adult primary ...
12
Infection of primary human fetal astrocytes by human herpesvirus 6.
... any primary neural cells is not ...purified primary fetal astrocytes in ...the astrocytes are infected by ...primary astrocytes. These findings suggest that infection of ...
5
Interferon Beta Contributes to Astrocyte Activation in the Brain following Reovirus Infection
... of primary astrocytes resulted in significant upregulation of cytokines that are associated with astrocyte activation ...of astrocytes, the expression of IL-6 and CCL5 increased ⬃ 15-fold, and the ...
12
Profiling the proteomic inflammatory state of human astrocytes using DIA mass spectrometry
... immortalized astrocytes (42 DDA runs) and a spectral library for the primary astrocytes (12 DDA runs) were ...of primary astrocytes, protein abun- dances were exported from Spectronaut, ...
14
CD4-Independent Entry and Replication of Simian Immunodeficiency Virus in Primary Rhesus Macaque Astrocytes Are Regulated by the Transmembrane Protein
... in primary macaque astrocytes, we analyzed the replication phenotype of four infectious molecu- lar clones in primary astrocyte cultures and further compared their replication kinetics to those in ...
8
Regulation of Proinflammatory Cytokine Expression in Primary Mouse Astrocytes by Coronavirus Infection
... In an attempt to identify the potential viral gene(s) respon- sible for the differential induction of proinflammatory cyto- kines, we used two chimeric viruses with an exchange of the S or N gene between MHV-A59 and ...
11
Role of β-Catenin and TCF/LEF Family Members in Transcriptional Activity of HIV in Astrocytes
... in primary astrocytes and astrocytomas transiently transfected with an HIV long terminal repeat (LTR)-luciferase reporter that contained an integrated copy of the HIV LTR-luciferase ...in astrocytes ...
11
Identification and molecular mechanisms of the rapid tonicity induced relocalization of aquaporin 4 channel
... Primary astrocytes suffer from poor transfection efficiencies and slow growth. HEK293 cells were therefore used to further investigate AQP4 subcellular relocalization because they are much more tractable ...
11
Original Article Dexmedetomidine inhibits lipopolysaccharide-in duced inflammatory response in hippocampal astrocytes in vitro
... University. Primary astrocytes of hippo- campus were prepared as previously described ...days, astrocytes were separated from microglia and oligodendrocytes by shaking for 18 h (200 rpm ...isolated ...
8
Cross-Interaction between JC Virus Agnoprotein and Human Immunodeficiency Virus Type 1 (HIV-1) Tat Modulates Transcription of the HIV-1 Long Terminal Repeat in Glial Cells
... same astrocytes (B; ...bizarre astrocytes (C; double labeling). (D to F) Primary astrocytes were coinfected with HIV-1 and JCV, and by immunofluorescence we detected Tat in both nuclei and ...
12
Sphingosine 1 phosphate receptors regulate TLR4 induced CXCL5 release from astrocytes and microglia
... In astrocytes, treatment with EGF, FGF-2 and insulin attenuates S1P-induced phospholipase C activation and phosphoinositide hydrolysis (Rao et ...in astrocytes, such that they increase levels of S1P (Riboni ...
12
A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells
... and astrocytes isolated from infected and sham- infected ...with astrocytes exhibiting higher levels than ...and astrocytes isolated from uninfected brain tis- sue showed detectable levels of RIP3 ...
12
Upregulation of mesencephalic astrocyte-derived neurotrophic factor in glial cells is associated with ischemia-induced glial activation
... Ischemia-induced MANF expression in oligodendrocytes The expression of MANF was also determined in oligo- dendrocytes in the present study. The myelin protein 2 0 3 0 -cyclic nucleotide 3 0 -phosphodiesterase (CNP) was ...
13
DNA Damage and Oxidative Stress Induced-p53 Activity In Astrocytes causes Growth Arrest
... the astrocytes were altering their proliferative abilities in response to the DNA damaging agents or oxidative ...of astrocytes in the treated wells compared to the untreated (Figure ...that ...
105
Borna Disease Virus Persistence Causes Inhibition of Glutamate Uptake by Feline Primary Cortical Astrocytes
... Viral genome and subgenomic NP mRNA were readily de- tected at 80 days p.i. (four cell passages, FeAst/BDVp4) by Northern blot hybridization (Fig. 2A). Likewise, BDV NP and P gene products were detected in FeAst/BDV ...
9
α7 nicotinic acetylcholine receptor-mediated neuroprotection against dopaminergic neuron loss in an MPTP mouse model via inhibition of astrocyte activation
... GFAP-IR astrocytes, and Mac-1-IR microglia in the substantia nigra pars compacta (SNpc) were counted from six mice per group using an optical fractionator [13], which is an unbiased method of cell counting that is ...
15
Nitric oxide and cytokine production by glial cells exposed in vitro to neuropathogenic schistosome Trichobilharzia regenti
... Additionally, stimulatory properties of T. regenti re- combinant cathepsins B1.1 and B2 were also tested in our experiments. These proteases are highly expressed in migrating schistosomula [50, 51] and can get in con- ...
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