primary hepatocytes
Construction of avian hepadnavirus variants with enhanced replication and cytopathicity in primary hepatocytes.
... While defects in cccDNA regulation were accompanied by a decrease of enveloped virus production in transfected cells, primary hepatocytes infected by such mutant viruses transiently prod[r] ...
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Comparative nucleic acid transfection efficacy in primary hepatocytes for gene silencing and functional studies
... After overnight incubation, plated primary hepatocytes were transfected with plasmid pRNAT-H1.1/neo con- taining a green fluorescent protein (GFP) expression cassette (GeneScript, Piscataway, NJ). Four ...
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Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes
... In this work, we engineered the naturally occurring, highly repli- cative HBV mutant 6898, which harbors a 207-bp deletion in the preS1/polymerase spacer region, into an efficient rHBV vector, 5c3c. A novel cargo gene ...
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Entry of Hepatitis B Virus into Immortalized Human Primary Hepatocytes by Clathrin-Dependent Endocytosis
... human hepatocytes provides a clue to the cause of the disordered metabolism of iron ...human primary hepatocytes could be performed to analyze differentially expressed genes and proteins responsible ...
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Fatty acids-stress attenuates gluconeogenesis induction and glucose production in primary hepatocytes
... Unlike key gluconeogenic enzymes, expression levels of main lipid metabolism-related genes CPT1 and GPAT were not affected. These results are in agreement with previous studies indicating that long-chain FAs have no ...
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Selective inhibition of liver X receptor α-mediated lipogenesis in primary hepatocytes by licochalcone A
... ChIP assays were performed on primary hepatocytes treated with 1 μM T0901317 and 10 μg/mL selected com- pound for 30 min, as described previously, with minor modifications [22]. Briefly, cells were fixed ...
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Insulin regulates the expression of several metabolism related genes in the liver and primary hepatocytes of rainbow trout (Oncorhynchus mykiss)
... phosphorylated to G6P by glucokinase (Granner and Pilkis, 1990) before being stored as glycogen, transformed into fatty acids or catabolized. Insulin is a major regulator of GK gene expression in the mammalian liver ...
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Effects of Environmental Contaminants and Natural Substances on Vitellogenesis in Tilapia Primary Hepatocytes
... a commercial phytoestrogens and plant extracts (fenugreek, FG; aniseed, AS; safflower, SF; flaxseed, FS; fennel, FN; liqurice, LR; pomegranate, PG; soybean, SB) as natural substances, respectively, and their estrogenic ...
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Hypoxic preconditioning potentiates the trophic effects of mesenchymal stem cells on co-cultured human primary hepatocytes
... of hepatocytes as BLK participates in pre-B-cell receptor-mediated activa- tion of nuclear factor kappa B [41], which tunes the balance between apoptosis and proliferation of hepato- cytes in response to TNF-α ...
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Transport of selenium across the plasma membrane of primary hepatocytes and enterocytes of rainbow trout
... as opposed to the linear uptake rate in hepatocytes. Sigmoidal transport kinetics indicates positive co-operativity at transport sites in enterocytes. The Hill coefficient derived from the kinetics data was almost ...
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Recombinant Duck Interferon Gamma Inhibits Duck Hepatitis B Virus Replication in Primary Hepatocytes
... the hepatocytes after infection the accumulation of viral DNA, especially ssDNA, was retarded, suggesting that DuIFN-g inhibited an early step in the virus replication ...
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Primary hepatocytes of Tupaia belangeri as a potential model for hepatitis C virus infection
... Tupaia hepatocytes, indi- cating that only a small fraction of hepatocytes synthesize detectable levels of HCV ...HCV-infected primary Tupaia hepato- cytes did not show any cytopathic changes ...
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N–nitrosodiethylamine cytochrome P450 induction and cytotoxicity evaluation in primary cultures of rat hepatocytes
... The primary routes of potential human exposure to N-nitrosodiethylamine (NDEA) are ingestion, inhala- tion, and dermal ...rat primary hepatocytes cultures, were used to understand the CYP induction ...
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Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent
... of primary hepatocytes weakly (60%) compared with HCVser infection of primary hepatocytes (92%), and JFH1/HCVcc infection of ...of hepatocytes, the difference in affinity or kinetics of ...
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TGFbeta induces apoptosis and EMT in primary mouse hepatocytes independently of p53, p21(Cip1) or Rb status
... One of TGFβ properties is to induce morphological changes associated with epithelial to mesenchymal transi- tion (EMT), which is characterised by downregulation of epithelial markers concomitant with upregulation of mes- ...
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Detection of nanolevel drug metabolites in an organotypic culture of primary human hepatocytes and porcine hepatocytes with special reference to a two-compartment model
... the hepatocytes before large-scale clinical ...of primary hepatocytes (human and pig), one can quickly identify the metabolite concentrations of drug candidates at different stages of the drug ...
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Scavenger Receptor Class B Type I and Hepatitis C Virus Infection of Primary Tupaia Hepatocytes
... human hepatocytes due to species-specific factors and SR-BI and CD81 have been shown to represent coreceptors for the entry of HCVpp into Huh-7 cells (5) and human hepatocytes (4), the findings obtained in ...
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Efficient Infection of Primary Tupaia Hepatocytes with Purified Human and Woolly Monkey Hepatitis B Virus
... We have previously reported that primary hepatocytes from T. belangeri can be infected with HBV (17). However, the practical use of this system was limited by the low efficiency of infection. Here, we show ...
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Assembly of Hepatitis Delta Virus: Particle Characterization, Including the Ability To Infect Primary Human Hepatocytes
... the hepatocytes for the limited extent of infection was to consider the infectivity of assembled ...both primary human hepatocytes and primary woodchuck hepatocytes (kindly provided by ...
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Primary Human Hepatocytes Are Susceptible to Infection by Hepatitis Delta Virus Assembled with Envelope Proteins of Woodchuck Hepatitis Virus
... In summary, the use of combinations of WHV and HBV envelope proteins did not interfere with the assembly of HDV RNA-containing particles. Further, these particles could be infectious on primary hepatocytes. ...
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