Primers, PCR amplification and analysis
Detection of Mycobacterium tuberculosis by PCR amplification with pan Mycobacterium primers and hybridization to an M tuberculosis specific probe
... The recent resurgence in tuberculosis (TB) cases poses a serious public health problem. Effective TB management re- quires the rapid detection and identification of the etiologic agent. The currently accepted ‘‘gold ...
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Amplification/PCR. Amplification Software 303
... predesigned primers and to design compatible oligonucleotide probes, extending the assay ...of primers and probes, all possible dimers, cross- dimers, and repeats, all in a graphical ...
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Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs
... DNA-based methods are perhaps the most promising ones and are now established as powerful tools for study- ing food chains [12]. Their advantage lies in enabling identification of prey when the remains are degraded or ...
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Yfiler Plus PCR Amplification Kit
... the primers Non-nucleotide linkers are used in primer synthesis for the DYS389I/II, DYS635, DYS627, DYS19, YGATAH4, DYS448, DYS391, DYS390, DYS438, DYS391, DYS390, DYS438, DYS392, DYS518, DYS437 and DYS449 ...
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AmpFlSTR Identifiler Direct PCR Amplification Kit
... these primers, non-nucleotide linkers are placed between the primers and the fluorescent dye during oligonucleotide synthesis (Butler 2005, Grossman et ...simultaneous amplification and efficient ...
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Characterization of Shigella Strains in Iran by Plasmid Profile Analysis and PCR Amplification of ipa Genes
... by PCR assay. The results of DNA amplification by the PCR method based on the primers used in this study showed the presence of a 619-bp fragment for the ipaH gene and a 612-bp fragment for ...
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PCR AMPLIFICATION AND EXPRESSION ANALYSIS OF P7 GENE OF HCV IN Huh-7 CELL LINE
... antisense primers were used to synthesis cDNA fragments by RT-PCR. PCR with Tm 60C and 240bp fragments containing p7 gene was used to amplify gene-specific ...the PCR prod- uct from agarose ...
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Computational analysis of stochastic heterogeneity in PCR amplification efficiency revealed by single molecule barcoding
... different primers, and secondary structure within target sequences are well-established causes of PCR ...which amplification bias can be attributed to sequence ...the amplification of ...
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Detection and Identification of Mycobacteria by Amplification of the Internal Transcribed Spacer Regions with Genus and Species Specific PCR Primers
... of PCR assays that target the internal transcribed spacer (ITS) region for identifying mycobacteria at the species ...multialignment analysis. One pair of panmycobacterial primers and seven pairs of ...
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Homogeneous Amplification of Genital Human Alpha Papillomaviruses by PCR Using Novel Broad Spectrum GP5+ and GP6+ Primers
... -globin PCR product ...the amplification sensitivity and the detection limit of the read-out ...HPV amplification and genotyping methods that allow specific, sensitive, unbiased, and robust detection ...
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A database of PCR primers for the chloroplast genomes of higher plants
... restriction analysis or DNA ...utilized PCR-amplified chloroplast gene sequences for establishing and verifying ...of primers recommended for this purpose have also expanded in size ...the ...
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A systematic guideline for developing the best real-time PCR primers
... curve analysis must verify that a single gene-specific product is ...the amplification efficiency must be greater than 90 percent for accurate and reliable ...
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Designing Of Degenerate Primers-Based Polymerase Chain Reaction (PCR) For Amplification Of WD40 Repeat-Containing Proteins Using Local Allignment Search Method
... The primers designed were amplified the putative WD40 repeat gene fragment of 249 bp from the cDNA template in the second round of ...of amplification gave multiple bands (almost obtained smear products ...
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Evaluation of Typing of Vibrio parahaemolyticus by Three PCR Methods Using Specific Primers
... clustering analysis indicated that these methods apparently differentiated ...these PCR methods closely approached or even exceeded those of PFGE and ...fewer amplification bands and patterns than ...
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Characterization and Identification of F1 Hybrids in Eucalyptus by PCR Study using Random Primers
... RESULTS AND DISCUSSION Out of the 17 decamer primers used for the RAPD analysis, only 6 primers viz. M-188, M-119, M-31, M-33, M-122 and M-191 yielded the best amplification products. The ...
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Polyadenylated Sequencing Primers Enable Complete Readability of PCR Amplicons Analyzed by Dideoxynucleotide Sequencing
... strand analysis, primer walking (18) or long-range PCR product sequencing (9) is necessary for a complete read- ...and primers after the sequencing reaction; ii) low separation efficien- cy of ...
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Impact of Enzymes and Primers on the PCR Amplification of Some Goatfishes
... of PCR amplification of target genes from genomic DNA (gDNA) of many environmental specimens (Schneider, Enkerli, & Widmer, ...of PCR, but normally inhibit the polymerase from amplifying the ...
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PCR primers Universal primers for PCR am-
... However, individual members within each group were not readily distinguished (VAN DEN HEUVEL et al. 1994), and other molecular methods have to be applied. TORDO et al. (1995) demonstrated that the 5'-end region of the ...
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Supplementary Table 1. Forward and reverse primers used in PCR amplification and cycle
... Bayesian phylogenetic trees estimated with partitioned models of sequence evolution by codons, where first, second and third position were.. estimated separately.[r] ...
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Pcr amplification by two different primers for early diagnosis of schistosoma mansoni infected snails
... for PCR is obligatory. In the present study, two types of primers for conventional PCR were used and compare between them and shedding, crushing) in attempt to apply this technique in the field for ...
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