Prion propagation
Schizosaccharomyces pombe Disaggregation Machinery Chaperones Support Saccharomyces cerevisiae Growth and Prion Propagation
... and propagation of [PSI ⴙ ] and [URE3] ...fragment prion polymers, we anticipated that ...[URE3] prion phenotypes in cells ex- pressing Ssa1p or Ssa2p as the only essential Ssa family Hsp70 showed ...
7
Primate Chaperones Hsc70 (Constitutive) and Hsp70 (Induced) Differ Functionally in Supporting Growth and Prion Propagation in Saccharomyces cerevisiae
... Hsp70’s are highly conserved essential protein chaperones that assist protein folding and prevent pro- tein aggregation. They have modular structures consisting of ATPase, substrate-binding, and C-terminal domains. ...
11
Prion Propagation and Toxicity Occur In Vitro with Two-Phase Kinetics Specific to Strain and Neuronal Type
... vitro prion propagation and neurotoxicity of three different scrapie strains (139A, ME7, and 22L) that had been stabilized in C57BL/6 mice and had demonstrated distinct lesion profiles ...strain ...
14
The β6/β7 region of the Hsp70 substrate binding domain mediates heat shock response and prion propagation
... Our finding that β6–β7 can act as a location for induc- ing structural changes and signals within the SBD is in agreement with recent data highlighting the importance of this region [49]. Meanwhile, our data further ...
16
Mutated but Not Deleted Ovine PrPC N-Terminal Polybasic Region Strongly Interferes with Prion Propagation in Transgenic Mice
... cellular prion protein ...mutant prion proteins and the efficiency of prion propagation, both in vitro and in ...on prion susceptibil- ity of minimal amino acid substitutions and ...
9
Mutational analysis of Sse1 (Hsp110) suggests an integral role for this chaperone in yeast prion propagation in vivo
... Both Fes1 and Sse1 have been shown to be NEFs for cytosolic Hsp70s (Kabani et al. 2002b; Dragovic et al. 2006; Raviol et al. 2006b) We therefore assessed the ability of Fes1 to complement the prion prop- agation ...
11
Prion Propagation in Cells Expressing PrP Glycosylation Mutants
... and prion propagation over several ...mouse prion-infected transgenic mice (38, 56), underscores the possibility that any effect observed on prion biology could involve the amino acid ...
9
Using steered molecular dynamics to predict and assess Hsp70 substrate binding domain mutants that alter prion propagation
... for prion impairment was proposed to be through an increased affinity for prion substrate exhibited by Ssa1- 21, which would interfere with the prion replication cycle ...impair prion ...
10
Cytosolic Prion Protein Toxicity Is Independent of Cellular Prion Protein Expression and Prion Propagation
... induce prion disease in mice by inoculation of cyPrP agrees with this ...typical prion disease in non-Tg mice inoculated with cyPrP derived from symptomatic cyPrP-expressing mice or with lysates prepared ...
7
Protein Folding Activity of the Ribosome is involved in Yeast Prion Propagation
... ous times in both HSP104 and hsp104Δ backgrounds. ltv1Δ and yar1Δ mutants proved more thermoresistant than WT strain (Fig. 1B), and also when combined with hsp104Δ versus hsp104Δ alone (Fig. 1C). Thus, PFAR enrichment ...
15
Mutational Analysis of the [Het-s] Prion Analog of Podospora anserina: A Short N-Terminal Peptide Allows Prion Propagation
... Strains and culture conditions: P. anserina is a filamentous al. 1997). This modification does not alter the electro- ascomycete. The mycelium has a coenocytic structure; cells phoretic mobility of the protein in ...
12
Paracrine Inhibition of Prion Propagation by Anti-PrP Single-Chain Fv Miniantibodies
... The bovine spongiform encephalopathy epidemic (42) can be arguably deemed the direct consequence of flawed technol- ogy assessment and is caused by prions that were amplified through the bovine food chain (41). ...
9
Efficient Lymphoreticular Prion Propagation Requires PrPc in Stromal and Hematopoietic Cells
... The nature of cells replicating prions in the periphery, and the precise role of Prnp expression in these cells, is still unclear. A likely candidate for a lymphoreticular prion replication site is the FDC (9, 22, ...
10
A Role for Cytosolic Hsp70 in Yeast [PSI+] Prion Propagation and [PSI+] as a Cellular Stress
... [PSI ⫹ ] is a prion (infectious protein) of Sup35p, a subunit of the Saccharomyces cerevisiae translation termination factor. We isolated a dominant allele, SSA1-21, of a gene encoding an Hsp70 chaperone that ...
12
Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro
... known to act as superior amplification sub- strates even to other prion types like BSE FIGURE 5. Inhibition of bovine BSE prion replication with rPrPs. Bovine BSE (SE1762/0013) was amplified in triplicates ...
13
Prokaryotic Chaperones Support Yeast Prions and Thermotolerance and Define Disaggregation Machinery Interactions
... promotes propagation of amyloid prions in yeast, but ClpB does neither, leading to a view that Hsp104 evolved these ...both prion propagation and thermotolerance in yeast if it is modified to interact ...
14
Analysis of the Generation and Segregation of Propagons: Entities That Propagate the [PSI+] Prion in Yeast
... The propagation of the prion form of the yeast Sup35p protein, the so-called [PSI ⫹ ] determinant, involves the generation and partition of a small number of particulate determinants that we propose calling ...
12
Gauczynski, Sabine (2002): Characterization of the 37-kDa/67-kDa laminin receptor as the cell surface receptor for the cellular prion protein. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... cellular prion protein (Gauczynski et ...glycosylated prion proteins in mammalian cells is essential for a better understanding of the physiological function of PrPc and biochemical processes responsible ...
199
PubMedCentral-PMC4774245.pdf
... Japanese prion surveillance work was supported by a grant-in- aid from the Research Committee of Prion Disease and Slow Virus Infection, the Ministry of Health, Labour and Welfare of Japan, and from the ...
22
Function of Prion Protein and the Family Member, Shadoo
... with prion disease show misfolded PrP accumulation and developed extensive neu- rodegeneration, in contrast to mouse models of Alzheimer’s disease (AD) or Parkinson’s disease (PD), in which neuronal loss is ...
22