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Proliferation assay

Optimisation of the lymphocyte proliferation assay in rainbow trout ( Oncorhynchus mykiss

Optimisation of the lymphocyte proliferation assay in rainbow trout ( Oncorhynchus mykiss

... lymphocyte proliferation assay is a valuable method used for the evaluation of the fish immune ...lymphocyte proliferation assay in rainbow trout (Oncorhynchus ...highest proliferation ...

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Next-generation sequence detects ARAP3 as a novel oncogene in papillary thyroid carcinoma

Next-generation sequence detects <em>ARAP3</em> as a novel oncogene in papillary thyroid carcinoma

... Methods: Whole exon sequence and whole genome sequence of primary papillary thyroid carcinoma (PTC) samples and matched adjacent normal thyroid tissue samples were per- formed and then bioinformatics analysis was carried ...

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Knockdown of a DIS3L2 promoter upstream long noncoding RNA (AC105461.1) enhances colorectal cancer stem cell properties in vitro by down-regulating DIS3L2

Knockdown of a DIS3L2 promoter upstream long noncoding RNA (AC105461.1) enhances colorectal cancer stem cell properties in vitro by down-regulating DIS3L2

... cell proliferation assay, matrigel invasion assay, and spheroid formation as well as flow cytometry analysis were performed respectively to investigate the effect of ...

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Long non-coding RNA Malat1 activated autophagy, hence promoting cell proliferation and inhibiting apoptosis by sponging miR-101 in colorectal cancer

Long non-coding RNA Malat1 activated autophagy, hence promoting cell proliferation and inhibiting apoptosis by sponging miR-101 in colorectal cancer

... cell proliferation and reduced apoptosis by activating autophagy Due to the low transfection efficiency of other cell lines, HCT116 and SW620 cell lines were used in this ...cell proliferation and ...

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LncRNA WT1-AS Downregulates Survivin by Upregulating miR-203 in Papillary Thyroid Carcinoma

<p>LncRNA WT1-AS Downregulates Survivin by Upregulating miR-203 in Papillary Thyroid Carcinoma</p>

... Compared to C and NC groups, cell proliferation assay showed that over- expression of WT1-AS and miR-203 overexpression led to decreased rates of cell proliferation, while survivin over-[r] ...

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XIST Induced by JPX Suppresses Hepatocellular Carcinoma by Sponging miR 155 5p

XIST Induced by JPX Suppresses Hepatocellular Carcinoma by Sponging miR 155 5p

... and proliferation assay was performed using CCK-8 reagent purchased from Dojindo (Shanghai, China) following the manufacturer’s ...formation assay were purchased from Jackson Laboratory (Bar Harbor, ...

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Gene Expression Comparison Between the Lymph Node-Positive and Negative Reveals a Peculiar Immune-microenvironment Signature and a Theranostic Role for WNT Targeting in Pancreatic Ductal Adenocarcinoma: A Pilot Study

Gene Expression Comparison Between the Lymph Node-Positive and Negative Reveals a Peculiar Immune-microenvironment Signature and a Theranostic Role for WNT Targeting in Pancreatic Ductal Adenocarcinoma: A Pilot Study

... of XAV-939 on two PDAC cell lines, PANC-1 and MIAPaCa-2, by CCK-8 proliferation assay.. The cell proliferation (%)/dose plots of the mean of 189.[r] ...

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Cordyceps bassiana inhibits smooth muscle cell proliferation via the ERK1/2 MAPK signaling pathway

Cordyceps bassiana inhibits smooth muscle cell proliferation via the ERK1/2 MAPK signaling pathway

... the proliferation of vascular smooth muscle cells (VSMC) and their underlying molecular ...cell proliferation assay showed that Cordyceps bassiana ethanol extract (CBEE) significantly inhibited VSMC ...

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Berberine inhibits NLRP3 Inflammasome pathway in human triple negative breast cancer MDA MB 231 cell

Berberine inhibits NLRP3 Inflammasome pathway in human triple negative breast cancer MDA MB 231 cell

... After treatment with various concentrations of BBR (2.5, 5, 10, 20, 40, 60, 80, 100 μg/ml) for 48 h, the cell viability was measured by the CellTiter 96® AQueous One Solu- tion Cell Proliferation Assay ...

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Original Article FL118 inhibited the proliferation of colorectal carcinoma cells via upregulating CDC73

Original Article FL118 inhibited the proliferation of colorectal carcinoma cells via upregulating CDC73

... MTT assay, scratch wound assay and BrdU cell proliferation assay were employed to test colorectal carcinoma cell viability, mobility and proliferation under the treatment of ...and ...

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Original Article MAPK1 up-regulates the expression of MALAT1 to promote the proliferation of cardiomyocytes through PI3K/AKT signaling pathway

Original Article MAPK1 up-regulates the expression of MALAT1 to promote the proliferation of cardiomyocytes through PI3K/AKT signaling pathway

... cell proliferation may be stimulated in the pathologic ...cardiomyocytes proliferation process. In this study, the proliferation assay and cell cycle assay showed the ...

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The Activity of Combination of Hydrolyzed Virgin Coconut Oil and Chitosan Toward Wound Healing Parameters on NIH 3T3 Cells Using in Vitro Methods

The Activity of Combination of Hydrolyzed Virgin Coconut Oil and Chitosan Toward Wound Healing Parameters on NIH 3T3 Cells Using in Vitro Methods

... cell proliferation assay was determined by MTT (Microculture Tetrazolium Technique) assay, cell proliferation activity was measured by calculating cell viability incubated 24 hours, wound ...

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Similar cisplatin sensitivity of HPV-positive and -negative HNSCC cell lines

Similar cisplatin sensitivity of HPV-positive and -negative HNSCC cell lines

... after cisplatin administration where marked differences in the cellular cisplatin responses were evident between cell lines of different cisplatin sensitivity. Using this setup cell survival will depend on both the ...

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Onco-miR-130 promotes cell proliferation and migration by targeting TGF R2 in gastric cancer

Onco-miR-130 promotes cell proliferation and migration by targeting TGF R2 in gastric cancer

... activity assay revealed that miR-130 mimics could inhibit luciferase activity in the wild-type 3′UTR of TGFβR2 mRNA but did not work in the mutant ...promote proliferation and migration of SGC7901 ...

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The long noncoding RNA PCGEM1 promotes cell proliferation, migration and invasion via targeting the miR-182/FBXW11 axis in cervical cancer

The long noncoding RNA PCGEM1 promotes cell proliferation, migration and invasion via targeting the miR-182/FBXW11 axis in cervical cancer

... as proliferation, migration, invasion, drug resistance, autophagy and angiogenesis [5, ...cell proliferation [14, ...cell proliferation, migration, and invasion, but decreased cell apoptosis through ...

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Deletion of low-density lipoprotein-related receptor 5 inhibits liver Cancer cell proliferation via destabilizing Nucleoporin 37

Deletion of low-density lipoprotein-related receptor 5 inhibits liver Cancer cell proliferation via destabilizing Nucleoporin 37

... promote proliferation of HCC cells via interacting with YAP and subsequently activating YAP/ TEAD signaling ...cell proliferation following knockdown of NUP37 in HepG2 ...

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DOCOSAHEXAENOIC ACID INDUCES APOPTOSIS  IN COLON CANCER CELL LINE, Caco-2

DOCOSAHEXAENOIC ACID INDUCES APOPTOSIS IN COLON CANCER CELL LINE, Caco-2

... MTT assay DNA fragmentation assay, cytological and immunocytochemical investigations were ...MTT assay was used to test DHA on the effect of the proliferation of the colorectal cancer cell ...

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Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function

Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function

... reference. The PCR primers used in this study were as follows: hsa-PTEN: forward, 5'-AGGGACGAACTGG TGTAATGA-3', and reverse, 5'-CTGGTCCTTACTTC CCCATAGAA-3'; hsa-SPRY1: forward, 5'-CCCACTG CTGACACAAGGAG-3', and reverse, ...

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Legumain Promotes Gastric Cancer Progression Through Tumor-associated Macrophages In vitro and In vivo

Legumain Promotes Gastric Cancer Progression Through Tumor-associated Macrophages In vitro and In vivo

... CCK-8 assay and transwell migration assay indicated that upregulation of LGMN expression in TAMs stimulated cell proliferation, migration and invasion in vitro, while downregulation of LGMN ...

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RETRACTED ARTICLE: The microRNA-325 inhibits hepatocellular carcinoma progression by targeting high mobility group box 1

RETRACTED ARTICLE: The microRNA-325 inhibits hepatocellular carcinoma progression by targeting high mobility group box 1

... by targeting Cyclin D1 and CDK4 [19]. The miRNAs can also predict the prognosis of patients with various cancers and may be a potential prognostic marker for cancers [20–22]. It was shown that the microRNA-125b inhibits ...

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