propidium iodide
Red Clover Necrotic Mosaic Virus: Biophysics and Biotechnology.
... The efficacy of a plant virus nanoparticle (PVN), loaded with a cargo (Dox or propidium iodide) and armed with a targeting peptide, was tested in vitro against severa[r] ...
239
Fluorometric Viability Assessment of Capacitated and Acrosome Reacted Boar Spermatozoa by Flow Cytometry
... [3,4]. Propidium iodide (PI) is a vital fluorescent dye that binds DNA in a non-covalent man- ner, indicating plasma membrane damage when cells emit red fluorescence ...
5
Abstract
... reference propidium iodide exclusion−test in estimating the toxic influ− ence of the anticancer drug doxorubicin (DOX, ...and propidium iodide exclusion ...
5
Pro-apoptotic versus anti-apoptotic properties of dietary resveratrol on tumoral and normal cardiac cells
... with propidium iodide (PI) shows that at both 30 and 100 lM, 50–70% of dead cells were found at 48 and 72 h in comparison with untreated cells (5–15%) ...
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Cell-permeable iron inhibits vascular endothelial growth factor receptor-2 signaling and tumor angiogenesis
... experiments. These results confirm that cell permeable iron, FAC, inhibits VEGF-induced endothelial cell proliferation. In order to investigate the mechanism of inhibition of VEGF-A induced cellular proliferation, we ...
16
Localization of Single- and Low-Copy Sequences on Tomato Synaptonemal Complex Spreads Using Fluorescence in Situ Hybridization (FISH)
... Fluorescence in situ hybridization (FISH) is a powerful means by which single- and low-copy DNA sequences can be localized on chromosomes. Compared to the mitotic metaphase chromosomes that are normally used in FISH, ...
14
Integrated holographic system for all optical manipulation of developing embryos
... To assess the success of optoinjection, P. lamarckii embryos of mixed cleavage stages were bathed in a solution of Propidium iodide (PI, Invitrogen) mixed in seawater to a final concentration of ~10 μM. PI ...
12
Development of a Novel System To Study Hepatitis Delta Virus Genome Replication
... Next, we examined the consequences of TET induction on the viability of 293-HDV cells. As mentioned earlier, prior to subcloning, we did observe detachment of some cells following several days of TET induction (Fig. 2 ...
7
Nuclear entry of hyperbranched polylysine nanoparticles into cochlear cells
... Hyperbranched polylysine nanoparticles (HPNPs) are dendritic polymers with imperfectly branched or irregular structures, which are polydispersed and can be prepared in a single step on a large scale. Studies of the ...
12
Barnacle biology before, during and after settlement and metamorphosis: a study of the interface
... SYTO 9/ propidium iodide staining showing antennules and the edge of the barnacle via autofluorescence blue, live bacteria green dots, dead bacteria red dots and patchy material green an[r] ...
14
TGF-β1 Increases Resistance of NIH/3T3 Fibroblasts Toward Apoptosis Through Activation of Smad2/3 and Erk1/2 Pathways
... with propidium iodide and increased annexin V binding (Figure 3A), indicative of ...of propidium iodide- and annexin V-positive cells was ...propidium iodide. Pretreatment of ...
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Repair of UVB-induced DNA damage is reduced in melanoma due to low XPC and global genome repair
... Cells were incubated in media containing BrdU (1:100, Life Technologies, USA) for 30 minutes; washed with PBS and resuspended in fresh media. At timepoints cells were fixed in ice cold 75% ethanol, washed with PBS + 50mM ...
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A novel small-form NEDD4 regulates cell invasiveness and apoptosis to promote tumor metastasis
... Stable cells were treated in the presence or absence of low -serum conditions medium with 1% serum for 48 h, followed by Annexin V-propidium iodide PI double staining, and the proportion[r] ...
14
Epi -reevesioside F inhibits Na /K -ATPase, causing cytosolic acidification, Bak activation and apoptosis in glioblastoma
... of propidium iodide staining revealed that both Epi-reevesioside F and ouabain induced concentration-dependent arrest of cell cycle at S and G2/M phases associated with apoptosis in T98 cells (Figure ...
15
Karyotype analysis of Lablab purpureus (L ) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes
... combined propidium iodide and 4',6-diamidino-2-phenylindole (CPD) staining and FISH with 5S and 45S rDNA probes: (a), (c), (e) – CPD banded prometaphase, metaphase and interphase cells, respectively; (b), ...
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Visualization of internalization of functionalized cobalt ferrite nanoparticles and their intracellular fate
... Cells were grown in a 24 well-plate (Corning Inc) and incu- bated with different concentration of NPs (50, 100, 150, and 200 µ g/mL) for 24 hours. After incubation, cells were gently washed three times with warmed PBS ...
13
SOX7 is down-regulated in lung cancer
... 2x10 6 cells stably expressing either SOX7 or GFP were seeded into 6-well plates for 24 h. Cells were harvested and washed twice with cold phosphate- buffered saline (PBS) and fixed in 75% ethanol (precooled at -20°C) ...
11
Role of CD133 in human embryonic stem cell proliferation and teratoma formation
... To obtain an overview of global changes, we subjected all of the dysregulated genes to KEGG analysis, and the results showed substantial enrichment for pathways in cancer (hsa05200). We also found the involvement of the ...
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Cell distribution after intracoronary bone marrow stem cell delivery in damaged and undamaged myocardium: implications for clinical trials
... Th e fi rst prerequisite for cell therapy success is the engraftment and thus, homing of transplanted cells to the Figure 5. Immunohistologic analyses. Representative organ sections, after intracoronary injection of ...
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Killing of human melanoma cells by the membrane attack complex of human complement as a function of its molecular composition
... and propidium iodide uptake; cell killing occurred in the absence of C9 with greater than 5 X 10(5) C5b-8/cell; the maximal molar ratio of C9:C8 was ...
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