Protease resistant
Affinity-Tagged Miniprion Derivatives Spontaneously Adopt Protease-Resistant Conformations
... The protease-resistant fragment of PrP106(140/6His) mi- grated on SDS-PAGE with an apparent molecular mass of 19 kDa ...the protease-resistant core of PrP106(225/6His) had an apparent ...
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Binding of EBNA-1 to DNA creates a protease-resistant domain that encompasses the DNA recognition and dimerization functions.
... Protease digestion generates an 18-kDa DNA-binding domain that maps within the aa 459 to 617 segment of EBNA-1, as determined by gel electrophoretic analysis of the protease-resistant EB[r] ...
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Protease resistant modified human β hexosaminidase B ameliorates symptoms in GM2 gangliosidosis model
... Recently, Cachón-González et al. demonstrated that the nature of the neurological injury in the SD mouse due to accumu- lation of glycoconjugates, lipids, α-synuclein, and ubiquitinated proteins — and induction of ...
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Formation of Protease-Resistant Prion Protein in Cell-Free Systems
... The central proposal of most protein-only models for the infectious agent of the TSEs is that the putative infectious protein, i.e. PrP-res, directly interacts with its normal, host encoded homolog, PrP-sen, to convert ...
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Scrapie-infected murine neuroblastoma cells produce protease-resistant prion proteins.
... The argument that PrPSC is an integral component of the infectious scrapie prion is now supported by seven distinct experimental observations: i PrP 27-30 and scrapie prions copurify; Pr[r] ...
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Serospecificity of a cloned protease resistant Treponema pallidum specific antigen expressed in Escherichia coli
... Distribution of bound 125I-labeled protein A detecting antigen 4D-specific antibody in normal patient sera and primary syphilis sera... phagedenis biotype Reiter, Treponema refringens,.[r] ...
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N-terminal truncation of the scrapie-associated form of PrP by lysosomal protease(s): implications regarding the site of conversion of PrP to the protease-resistant state.
... The activity of the anti-peptide 23-37 was demonstrated by the labeling of a subset of bands from a partially proteinase K-digested preparation of mouse brain PrP-res which contained bot[r] ...
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Rüßmann, Florian (2013): The eukaryotic chaperonin TRiC domain-wise folding of multi-domain proteins. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... PK treatment of hSnu114-N resulted in the formation of two major fragments of approximately 45 kDa and 55 kDa, regardless of whether AlFx was present or not (Figure 33a). Similar protease-resistant ...
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Deficiency of the exportomer components Pex1, Pex6, and Pex15 causes enhanced pexophagy in Saccharomyces cerevisiae.
... Figure 1. pex1Δ, pex6Δ, and pex15Δ cells show increased pexophagy that is dependent on the pexophagy receptor atg36. (A) Oleate grown cells (0) were shifted to nitrogen starvation conditions for 6 h (6) and pexoph- agy ...
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Trypanosoma congolense Procyclins: Unmasking Cryptic Major Surface Glycoproteins in Procyclic Forms
... a protease-resistant surface molecule (PRS), which is expressed by procyclic forms early in infection, and a glutamic acid- and alanine-rich protein (GARP), which appears at later ...
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Alzheimer's disease Aß assemblies mediating rapid disruption of synaptic plasticity and memory
... Cellular prion protein and Aβ-mediated disruption of synaptic plasticity and learning Given the likely key pathogenic role of a partially protease-resistant misfolded form of PrPC PrPSc,[r] ...
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Binding of the protease-sensitive form of PrP (prion protein) to sulfated glycosaminoglycan and congo red [corrected]
... Since our hypothesis is that sulfated glycans and Congo red inhibit protease-resistant PrP accumulation in scrapie-infected cells by competitively blocking an interaction of PrP with a c[r] ...
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MR Imaging of the Brain in Wilson Disease of Childhood: Findings Before and After Treatment with Clinical Correlation
... Such defects would result in a protease-resistant form of ceruloplasmin to be secreted into the blood and also excreted into the bile.1 The neurologic symptoms associated with Wilson dis[r] ...
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Molecular Characterization of Clinical Isolates of Human Immunodeficiency Virus Resistant to the Protease Inhibitor Darunavir
... In vitro selection studies suggest that development of resis- tance against darunavir requires more individual mutations and develops more slowly than development of resistance to other PIs (9). A close structural ...
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Altered Substrate Specificity of Drug-Resistant Human Immunodeficiency Virus Type 1 Protease
... I84V substitution, and HIV PRs 945 and 1049 each had a V82A substitution. Although residues 82 and 84 form part of the S1 pocket and not the S2 pocket, a reductive mutation at either position could cause a shift in bound ...
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Selection of Drug-Resistant Feline Immunodeficiency Virus (FIV) Encoding FIV/HIV Chimeric Protease in the Presence of HIV-Specific Protease Inhibitors
... Gag processing and DRV and TL-3 inhibitor sensitivities of NC-G37D mutant. A pCFIV ⌬ orf2 ⌬ env Gag-Pol expression vec- tor carrying NC-G37D was constructed to determine whether the NC-G37D mutation alters Gag processing ...
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Processing of the Bovine Spongiform Encephalopathy-Specific Prion Protein by Dendritic Cells
... lenged with BSE-infected BMDC did not develop BSE. These data suggest that BMDC do not release infection to nerve terminals and thus that these cells do not play a role in direct neuroinvasion. These results are in ...
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An antibiotic, heavy metal resistant and halotolerant Bacillus cereus SIU1 and its thermoalkaline protease
... good protease activity which was reassessed by loading their culture broth in the wells on milk agar plates (pH ...good protease producers cleared more than ...
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Replicative Fitness of Protease Inhibitor-Resistant Mutants of Human Immunodeficiency Virus Type 1
... different protease inhibitors (PIs) in vivo was ...nelfinavir-selected protease D30N substitution substantially decreased replicative capacity relative to WT, while a saquinavir- selected L90M substitution ...
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Amino Acid Insertions near Gag Cleavage Sites Restore the Otherwise Compromised Replication of Human Immunodeficiency Virus Type 1 Variants Resistant to Protease Inhibitors
... the protease and Gag proteins have been reported to contribute to the development of human immunodeficiency virus type 1 (HIV-1) resistance to protease ...wild-type protease was impaired by the ...
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