Protein Arrays
Increasing the sensitivity of reverse phase protein arrays by antibody-mediated signal amplification
... phase protein arrays (RPPA) have proven themselves as useful experimental platform for the validation of biomarker candidate proteins in biologi- cal and clinical samples ...specific protein or a ...
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Use of Miniaturized Protein Arrays for Escherichia coli O Serotyping
... miniaturized protein arrays increases the speed and efficiency of O serotyping in a cost-effective manner, and these preliminary findings suggest the microarray approach may have a higher accuracy than ...
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Spatiotemporal expression profiling of proteins in rat sciatic nerve regeneration using reverse phase protein arrays
... gamma-1 protein expression increased sig- nificantly between day 0 and day 28 both proximally and distally, and there was an early return to baseline gamma-1 levels in the guide ...
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Circulating and synovial antibody profiling of juvenile arthritis patients by nucleic acid programmable protein arrays
... nated protein), which are highly specific for RA, can only be detected in RF-positive JIA patients [12,13], but are associated with a higher risk of joint erosion, more aggressive disease and therapeutic ...
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Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions
... Akt protein was purchased from Invitrogen (cat# ...-20°C. Protein concentration was measured using Brad- ford assay (Bio-Rad, cat# ...the arrays. However, to correct for protein loading, Sypro ...
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Reverse phase protein arrays in signaling pathways: a data integration perspective
... Abstract: The reverse phase protein array (RPPA) data platform provides expression data for a prespecified set of proteins, across a set of tissue or cell line samples. Being able to measure either total proteins ...
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An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays
... scaled protein concentrations were normalized by global sample median normalization (GSMN) (the median of all protein marker intensities for a single sample is subtracted from each of the data points of ...
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RNAi-based validation of antibodies for reverse phase protein arrays
... total protein concentration was adjusted to 3 μ g/ μ l for samples ...total protein concentration per spot a slide from each print run was stained with Fast Green FCF (Sigma-Aldrich, Steinheim, Germany) as ...
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Multiplexed Nucleic Acid Programmable Protein Arrays
... To decrease the cost of high throughput screening experiments, we hypothesized that the plasmid cDNA encoding for different proteins could be multiplexed (by combining M different plasmids) within each feature to create ...
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Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
... Ordered arrays of peptides and proteins provide the basis of another strategy for parallel protein ...reviews). Protein assays using ordered arrays have been explored since the development of ...
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Hospital derived antibody profiles of malaria patients in Southwest India
... NAI is mediated by acquired immune mechanisms, primarily directed against blood stages of malaria para- sites. Such immunity involves circulating immunoglob- ulins, antibody-producing plasma cells, and memory B cells ...
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Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model
... cloning, protein expres- sion, immunization of rabbits, and affinity purification to yield polyclonal antibodies were performed as described previously ...All protein fragments used for immuni- zation were ...
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Diversity of matriptase expression level and function in breast cancer
... this protein in breast cancers remains relatively ...the protein level in multiple established human breast cancer cell lines using reverse phase protein arrays (RPPA) ...this protein ...
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Comprehensive protein tyrosine phosphatase mRNA profiling identifies new regulators in the progression of glioma
... Protein tyrosine phosphorylation is controlled via bal- anced actions of protein tyrosine kinases and protein tyrosine phosphatases (PTPs) [13]. Tumor cell biological research has mostly focused on ...
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Steroid Receptor RNA Activator Protein (SRAP): a potential new prognostic marker for estrogen receptor positive/node negative/younger breast cancer patients
... Further studies are urgently needed to establish the nature of the different splicing events potentially involved in the genera- tion of the different SRA transcripts and their potential protein products. This is ...
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Epitope spreading to citrullinated antigens in mouse models of autoimmune arthritis and demyelination
... GenePix Pro 5.0 software (Axon Instruments, Union City, CA, USA) was used to determine the median pixel intensity for each feature on the array. Each feature contained four to 12 replicates so the final intensity ...
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Lec8 - Intro to Arrays.pdf
... Arrays as function arguments, return values Arrays in recursive functions (next time!!). 5-2 Dr.[r] ...
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Distinct chemotaxis protein paralogs assemble into chemoreceptor signaling arrays to coordinate signaling output
... membrane-anchored arrays, we next determined the receptor classes composing each of the membrane-bound arrays in WT, ⌬ che1, and ⌬ che4 ...their protein sequence, specifically the heptad class of the ...
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Original Article Proteomic Profiling of Hepatitis B Virus-related Hepatocellular Carcinoma in China: a SELDI-TOF-MS Study
... of protein and peptide to those obtained by other proteomic methods, but we did not know what kind of protein they ...a protein based only on its molecular weight, and the most precise method is ...
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Probing the Sites of Interactions of Rotaviral Proteins Involved in Replication
... binding sites on NSP2 that map near the two-fold symmetry axis of the octamer extending into the catalytic cleft. Reciprocal pep- tide array screening with NSP2 sequence and binding analysis us- ing a full-length VP2, ...
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