Protein Microarrays
Application of sector protein microarrays to clinical samples
... in protein-based signaling pathways may lead to the hallmarks of cancer, which are exuberant growth, invasion, and metastatic spread ...the protein microarray is its ability to directly delve into the ...
9
Glycoprotein analysis using protein microarrays and mass spectrometry
... involves protein microarrays. Microarrays have proved to be a high- throughput technique because they allow multiple analytes to be investigated simultaneously due to their physical attachment to ...
15
Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
... of protein microarrays for specific detection and quantitation of multiple proteins in complex ...mixtures. Microarrays were constructed by printing microscopic spots of either antibodies (to detect ...
22
Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
... reviews). Protein assays using ordered arrays have been explored since the development of multipin synthesis [10] and spot synthe- sis [11] of peptides on cellulose ...supports. Protein arrays on membranes ...
13
ARTD10 substrate identification on protein microarrays: regulation of GSK3β by mono-ADP-ribosylation
... Background: Although ADP-ribosylation has been described five decades ago, only recently a distinction has been made between eukaryotic intracellular poly- and mono-ADP-ribosylating enzymes. Poly-ADP-ribosylation by ...
11
Development of reverse phase protein microarrays for the validation of clusterin, a mid-abundant blood biomarker
... measure protein levels in a high-throughput fashion is the protein microarray ...putative protein biomarkers in a single test sample ...phase protein microar- rays enable the high-throughput ...
12
Development of multiplexed protein profiling and detection using near infrared detection of reverse-phase protein microarrays
... as protein phosphorylation that are the drivers of the cellular signaling ...and protein phosphorylation of various signaling path- ways in small quantities of tumor tissue would be beneficial for both the ...
9
Determination of Specific Antibody Responses to the Six Species of Ebola and Marburg Viruses by Multiplexed Protein Microarrays
... induced protein expression was confirmed through Western blotting or mass spectrometry and Coomassie ...Bound protein was eluted using 25 mM HEPES, ...the protein did not appear to bind to the ...
8
Quantitative analysis of proteins of metabolism by reverse phase protein microarrays identifies potential biomarkers of rare neuromuscular diseases
... final protein concentra- tion of 1 μg/μl before ...diluted protein extracts (0–1 μg/μl) derived from HCT116 colocarci- noma cells were also prepared to asses printing quality and the linear response of ...
11
Signal stability of Cy3 and Cy5 on antibody microarrays
... or protein microarrays is ...cDNA microarrays have been conducted [30-35], it is not known whether amine-cou- pled fluorescent dyes on antibody/protein microarrays behave in the same ...
6
A perspective on microarrays: current applications, pitfalls, and potential uses
... including protein microarrays and tissue microarrays ...spotted microarrays and oligonucleotide microarrays ...Spotted microarrays are often referred to as dual-channel or ...
14
Microarrays Data Analysis for Cancer Disease on a Cluster of Computers
... of protein expression, Prediction of protein structure, and so on ...DNA microarrays [3], MMChips [4], Protein microarrays [5], Tissue microarrays [6], Cellular ...
8
Electrical protein detection in cell lysates using high-density peptide-aptamer microarrays
... most protein microarrays are produced by dot printing and consist of surface-immobilized antibodies; interactions with sample ligands are detected optically ...in protein arrays, and although likely ...
11
Probe Design and Data Analysis for Gene Expression Microarrays
... As DNA microarray technology becomes more adapted to large scale genome wide transcrip- tion profiling experiments, there has been an increasing need for rigorous experimental designs and proper data normalization ...
123
Characterization of adjacent breast tumors using oligonucleotide microarrays
... We tested the capabilities of oligonucleotide microarrays in determining tumor clonality. We obtained samples from adjacent tumors from a single individual, and compared the transcriptional profile of each tumor. ...
8
Linear model for fast background subtraction in oligonucleotide microarrays
... Results: We propose here an algorithm for background estimation based on a model in which the cost function is quadratic in a set of fitting parameters such that minimization can be per[r] ...
15
Tissue microarrays: one size does not fit all
... tissue microarrays (TMAs) have become a commonly-used research tool to evaluate asso- ciations between biomarkers and clinicopathologic tumor features, patient outcomes and treatment ...
10
The promise of microarrays in the management and treatment of breast cancer
... as microarrays capable of simultaneously measuring thousands of genes or gene products, we are beginning to construct molecular fingerprints of individual tumors so that accurate prognostic and predictive assess- ...
5
Evaluation of normalization methods for two channel microRNA microarrays
... Normalization is a key early step in miR microarray data processing. Normalization methods are aimed at removing data artifacts resulting from systematic or ran- dom technical variation. If not removed, these artifacts ...
7
Designing DNA Microarrays with Ant Colony Optimization
... DNA microarrays, also known as biochips, have applications in genetics, molecular biology, medicine, and pharmacology for detecting genes, diagnosing diseases, inventing drugs, toxicological analyses ...
9