PTEN promoter
The polycomb group protein EZH2 induces epithelial–mesenchymal transition and pluripotent phenotype of gastric cancer cells by binding to PTEN promoter
... of PTEN. Then, we determined whether Ezh2 regulates PTEN at the transcriptional level, our qRT-PCR results showed that aberrant expression of Ezh2 indeed affected PTEN mRNA expression; the mRNA ...
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Evaluating the Role of PTEN Promoter Methylation in Patients Predisposed to Hypercoagulable States via Methylation Specific PCR
... the PTEN gene promoter was discovered in metabolic syndrome (MeS), one of the risk factors for type 2 diabetes ...that PTEN promoter methylation status may be a protective factor in ...
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The prognostic effect of PTEN expression status in colorectal cancer development and evaluation of factors affecting it: miR-21 and promoter methylation
... the PTEN promoter methylation might be cancer specific and plays a role in the development of these ...expression PTEN mRNA in tumor and methylated samples com- pared with non-tumor and unmethylated ...
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Original Article Quantitative DNA hypomethylation of PTEN in Uyghur patients with type 2 diabetes mellitus
... the promoter regions were one amplicon, as previously reported ...the PTEN promoter region by EpiDesigner software ...T7 promoter tag was added for in vivo transcrip- tion, and a 10-mer tag ...
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PML/RARa inhibits PTEN expression in hematopoietic cells by competing with PU.1 transcriptional activity
... protein PTEN, as compared to blast cells from other AML subtypes or normal bone marrow, and demonstrate that PML-RARA directly inhibits PTEN ...the PTEN promoter, that allows the binding of ...
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Variants on the promoter region of PTEN affect breast cancer progression and patient survival
... with PTEN promoter variants develop breast tumors with differential gene expression signature We used microarray expression analysis to compare gene expression patterns in breast tumors from 10 patients ...
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Elevation of methylated DNA in <em>KILLIN</em>/<em>PTEN</em> in the plasma of patients with thyroid and/or breast cancer
... from either ethylenediaminetetraacetic acid blood or plasma samples was digested in a 40 µ L reaction volume with 30 U of the methyl ation-sensitive restriction enzyme, BstU1 (New England BioLabs, Hitchin, UK) at 60 ° C ...
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The association of <em>PTEN</em> hypermethylation and breast cancer: a meta-analysis
... of PTEN promoter hypermethylation was significantly increased in ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC) compared to normal breast tissues (OR ...of PTEN promoter ...
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miR-26a and miR-214 down-regulate expression of the PTEN gene in chronic lymphocytic leukemia, but not PTEN mutation or promoter methylation
... of PTEN expression. The methylation status of the PTEN promoter was evaluated in 75 untreated CLL patients and 25 healthy control ...low PTEN level. It seemed that the level of PTEN was ...
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<p>ZNF277 regulates ovarian cancer cell proliferation and invasion through inhibition of PTEN</p>
... 4 PTen was a direct downstream target of ZnF277 in Oc ...the promoter of ...P53, PTen, cDh1, and ...wild-type PTen promoter were examined in sKOV3 or OVcar3 cells transfected with ...
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Original Article Expression of NF-κB and PTEN in primary epithelial ovarian carcinoma and the correlation with chemoresistance
... embedded in paraffin, with consecutive 5 μm slices obtained from the same section. The slices were dewaxed by xylene, followed by gra- dient hydration with ethanol, and then pro- cessed in 3% hydrogen peroxide solution ...
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The drug-resistance to gefitinib in PTEN low expression cancer cells is reversed by irradiation in vitro
... of PTEN protein [18], H-157 cell line is the precondition of the ...of PTEN present in H-157 cells ...the PTEN expression, the negative regulator of the phosphatidylinositol 3-kinase (PI3K) pathway ...
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RUNX2 overexpression and PTEN haploinsufficiency cooperate to promote CXCR7 expression and cellular trafficking, AKT hyperactivation and prostate tumorigenesis
... the promoter (marked by high level of H3K4me3, a promoter histone marker) and the other in the enhancer (indicated by high level of H3K4me1, an enhancer histone marker) located in the first intron of CXCR7 ...
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microRNA-93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells
... of PTEN was predicted, using the algorithms in BLAST and ...of PTEN genes by binding to 3′ UTR of ...dogenous PTEN expression, we transfected the miR-93 and miR-93 mutant oligonucleotides, as well as ...
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Identification of a Movement Protein of the Tenuivirus Rice Stripe Virus
... P]UTP and T7 RNA polymerase according to instructions provided by the manufacturer (Takara, Dalian, China). The contaminating cDNA templates were removed through DNase digestion according to instructions provided by the ...
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Original Article Curcumin promotes apoptosis of breast cancer cells by down-regulating DJ-1-PTEN/PI3K/AKT signaling pathways
... from Takara (China). Cur was purchased from Sigma (US). Edu flow cytometry test reagent was purchased from RioBio (China). Rabbit anti-human DJ-1, AKT, p-AKT and β-actin poly- clonal antibodies were purchased from Abcam ...
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In renal cell carcinoma the PTEN splice variant PTEN-Δ shows similar function as the tumor suppressor PTEN itself
... of PTEN-Δ we aimed to create PTEN-Δ overexpressing RCC cells, which endogenously express low level of ...of PTEN-Δ and PTEN by qRT-PCR in the RCC cell lines A498, 786-O, Caki-1, Caki-2, ...
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PTEN gene mutations correlate to poor prognosis in glioma patients: a meta-analysis
... We investigated the relationship between PTEN genetic mutation and the prognosis of patients with glioma based on the data extracted from the studies included in this meta- analysis. For the time-to-event ...
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Targeting ataxia telangiectasia mutated and Rad3 related kinase (ATR) in PTEN deficient breast cancers for personalized therapy
... that PTEN deficient breast cancer cells were sensitive to ATR inhibition and was associated with accumulation of DSBs, cell cycle arrest and induction of ...that PTEN-deficient cells with impaired DNA ...
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Direct association of Bazooka/PAR 3 with the lipid phosphatase PTEN reveals a link between the PAR/aPKC complex and phosphoinositide signaling
... with PTEN to control the activity of the downstream kinase PKB/Akt (Wu et ...of PTEN may be important for its biological ...of PTEN lacking the PDZ-binding ...of PTEN in several biological ...
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