Pyrococcus furiosus
Biochemical and biophysical characterization of compartmentalizing proteases from the hyperthermophilic microorganism Pyrococcus furiosus
... Pyrococcus furiosus is a hyperthermophilic archaeon from the order Thermococcales that is capable of growth on a variety of proteinaceous and carbohydrate-containing ...P. furiosus cell extracts ...
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Functional Evaluation of the Mechanisms of Reactive Oxygen Detoxification in the Hyperthermophilic Archaeon Pyrococcus Furiosus Using Complementation Studies
... anaerobe, Pyrococcus furiosus, has an oxygen detoxification ...P. furiosus, the reactive oxygen specie superoxide is reduced to hydrogen peroxide by the enzyme superoxide reductase ...P. ...
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Functional Analysis of Tungsten Metabolism in Pyrococcus furiosus
... in Pyrococcus furiosus through a functional genomics ...P. furiosus has been a model organism for research on hyperthermophilic proteins and its genome has been ...P. furiosus are still ...
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Superoxide Reductase from the Hyperthermophilic Archaeon Pyrococcus furiosus: its Function, Regulation, and Biotechnological Applications
... Pyrococcus furiosus is a strict anaerobic hyperthermophilic archeaon that lives in both shallow and deep sea hydrothermal vents ...P. furiosus can periodically be exposed to oxygen in their ...
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Lammens, Alfred (2007): Structural and biochemical studies on the structural maintenance of chromosomes protein from Pyrococcus furiosus. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... SMC proteins involved in the SMC complexes, too (Haering et al., 2004). Scc1 serves in the cohesin complex as a bridge between the head domains of SMC1 and SMC3 and is cleaved by a protease named separase on anaphase ...
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Divergence of the Hyperthermophilic Archaea Pyrococcus furiosus and P. horikoshii Inferred From Complete Genomic Sequences
... Archaea, Pyrococcus furiosus and Pyrococcus horikoshii, was assessed by analysis of complete genomic sequences of both ...P. furiosus genome ...both Pyrococcus genomes, and two intein ...
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Functional Genomics Investigation of Microbial Physiology in the Hyperthermophilic Microorganisms Pyrococcus furiosus and Thermotoga maritima
... Full genome cDNA microarrays were used to assess differential gene expression patterns in the hyperthermophilic archaeon Pyrococcus furiosus grown on a range of glucose- based carbohydr[r] ...
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Development of the Extreme Thermophiles Pyrococcus furiosus and Sulfolobus acidocaldarius as Metabolic Engineering Platforms.
... genes in P. furiosus, has been most commonly used (297). A cold-induced promoter and a promoter activated under non-sulfur conditions have also been successfully used (155, 156). A promoter and RBS 295 bp upstream ...
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Functional Characterization of MoeA and MoeB Tungsten Cofactor Synthesis Proteins from the Hyperthermophilic Archaeon Pyrococcus furiosus
... P. furiosus MoeA, MoeA2, and MoeB ...P. furiosus proteins, cells from each expression were lysed and then heat-treated to denature the majority of the ...
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Transcriptomic and Physiological Analysis of a Recombinant Pyrococcus furiosus Strain Metabolically Engineered to Produce 3-Hydroxypropionate from CO2 and Maltose.
... reveal two sub-classes: a minor sub-class ‘Cam’ represented by CAM, and a major sub-class with conservation of CAM residues essential for metal binding and catalysis, except for Glu62 and the acidic loop residues, ...
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X Ray Crystallographic Studies on Electron Transfer Proteins; Rubredoxin from Pyrococcus furiosus, Nitrogenase MoFe from Azotobacter vinelandii and Ru(2,2' bppy)₂(imd)His83 Azurin from Pseudomonas aeruginosa
... Refinement of the oxidized form 414 protein atoms and 104 solvent oxygens with TNT or XPLOR resulted in a crystallographic residual of approximately 17% and a model with rms deviations o[r] ...
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Griese, Julia Johanna (2010): Structures and DNA-Binding Activities of the Hinge Domains from the Structural Maintenance of Chromosomes Proteins of Pyrococcus furiosus and the Mouse Condensin Complex. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... Although the mouse SMC2-SMC4 hinge heterodimer adopts an open conformation in the crystal structure described here, dimerising via only one of the two expected interfaces, the subunit cores themselves closely resemble ...
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Heterogeneity of proteinases from the hyperthermophilic archaeobacterium, Pyrococcus furiosus.
... P. furiosus can grow on a variety of complex substrates such as peptone or tryptone as well as on simpler carbon substrates, such as maltose (section ...P. furiosus can grow on peptides and proteins as sole ...
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Characterization of the Dinuclear Metal Center of Pyrococcus furiosus prolidase and Production of its Mutants with Increased Activity at Low Temperatures
... Using the positive selection strategy we designed, we successfully screened two cold-adapted P. furiosus prolidase variants, G39E- and E236V-prolidases, which had significant increased activity at low ...
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Investigations into the enzymology and biotechnology of the hyperthermophilic carboxypeptidase (PfuCP) from the Archaeon Pyrococcus furiosus
... The broad amino acid specificity and rapid digestion by PfuCP in peptide sequencing trials show promise, and high-temperature protein sequencing has now been demonstrated for the first t[r] ...
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Discovery, Functional Genomics and Biochemical Characterization of Alpha-specific Glycosyl Hydrolases from Hyperthermophilic Microorganisms
... Bioinformatic analysis, in conjunction with whole-genome transcriptional profiling, was used to identify ORFs with potential roles in α-glucan processing in the hyperthermophilic archaeon Pyrococcus ...
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Cell-Free Transcription at 95°: Thermostability of Transcriptional Components and DNA Topology Requirements of Pyrococcus Transcription
... Cell-free transcription of archaeal promoters is mediated by two archaeal transcription factors, aTBP and TFB, which are orthologues of the eukaryotic transcription factors TBP and TFIIB. Using the cell- free ...
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Modeling and structural analysis of PA clan serine proteases
... (Pyrococcus furiosus), 1 fungus (Neu- rospora crassa) and 1 plant (Arabidopsis thaliana) were obtained from the MEROPS protease database (http:// ...
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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
... Standard strain of (DSM 3773) Pyrococcus woesei were obtained from (DSMZ, Braunschweig, Germany). This kind of bacteria was routinely grown on batch mode using complex medium supplemented with sea water Marine ...
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Functional Genomics Analysis of Extremely Thermophilic Fermentative Microorganisms from the Archaeal Genus Pyrococcus and Bacterial Genus Caldicellulosiruptor.
... P. furiosus has a metabolic pathway to utilize, were unsuccessful, suggesting ...P. furiosus is auxotrophic for one or more amino acids (Watrin ...on Pyrococcus species identified valine and ...
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