Quantitative polymerase chain reaction assay
Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... Multiplexing primers and probes into one reaction or 2 reactions have been published also (Chmielewicz B et al 2005; Gu Z et al 2003; Ebner K et al 2006; Ebner K et al 2005). All these assays have been proved to ...
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Measurement of bioprocess containment by quantitative polymerase chain reaction
... are developed, it will simply require comparison o f the relative quantities of plasmid and chromosome. Notably, the QPCR assay for the chromosome of E. coli K-12 strains could be used for a number of different ...
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Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... or quantitative polymerase chain reaction (qPCR) in confirming the diagnosis of tubercular ...qPCR assay using primers targeting the MPB64 gene of Mycobacterium tuberculosis (MTB) was ...
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Development of a fluorescent quantitative real time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
... GPV detection by real-time PCR has only been reported by Bi [20]; in that study, the method was not optimized and a FQ-PCR standard curve was not generated. In this study, we reported the optimization of a FQ-PCR ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... LNA/Zip nucleic acids One critical factor for any PCR based detection platform, including RT-qPCR, is the invariant ability of the primer/ probe to discern its target from a heterogeneous mixture and bind it with extreme ...
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Original Article Novel real-time polymerase chain reaction assay for quantitative detection of Chlamydia trachomatis in vaginal and cervical secretions
... Quantitative detection in cell culture superna- tant and the mouse model Cell culture supernatants and mouse model specimens containing Chlamydia EBs were used to verify the feasibility of Chlamydia EB detection ...
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Establishment of real time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene
... time quantitative PCR became an attractive method to study gene expression because of its low inter-assay and intra- assay variablilty and its equivalent or greater analytical sensitivity in ...
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Detection of human herpesvirus 8 by quantitative polymerase chain reaction: development and standardisation of methods
... new quantitative assay including: laboratory establish- ment, choice of positive and negative controls, and assay optimisation and validation for HHV-8 ORF73, HHV-8 ORF26, and the human reference ...
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Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
... transcription, assay design, meas- urement of genomic contamination, standard curve data gen- eration, reference gene selection, and data normalisation were presented in the 'Materials and methods' and 'Results' ...
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Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
... transcription, assay design, meas- urement of genomic contamination, standard curve data gen- eration, reference gene selection, and data normalisation were presented in the 'Materials and methods' and 'Results' ...
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Analysis of Specific Bacteria from Environmental Samples using a Quantitative Polymerase Chain Reaction
... This article describes two QPCR assays. The internal standard QPCR produces accurate estimates of the amount of specific DNA sequences in the sample. It does not require sophisticated equipment but it does, however, ...
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Antiadenoviral effects of ganciclovir in types inducing keratoconjunctivitis by quantitative polymerase chain reaction methods
... Cytotoxicity assay The cytotoxicity of ganciclovir was evaluated in A549 ...This assay was carried out according to our previous ...ric assay for cell viability according to the manufacturer’s ...
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Development of a polymerase chain reaction assay for the rapid detection of the oral pathogenic bacterium, Selenomonas noxia
... Patricia Cruz 1* , Arthuro M. Mehretu 2,3 , Mark P. Buttner 1 , Theresa Trice 1 and Katherine M. Howard 4 Abstract Background: In recent studies, periodontal health has been linked to being overweight and/or obese. Among ...
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SYBR green based one step quantitative real time polymerase chain reaction assay for the detection of Zika virus in field caught mosquitoes
... Therefore, assays optimized for human samples may need to be modified, re-developed and evaluated before being used for pathogen surveillance in vector populations. This is notably applicable to assays with detection ...
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Polymerase chain reaction assay for avian polyomavirus
... Lanes 2, 3, 5, 7, and 9 contain the amplified products from purified DNA samples from CEFs infected with BFDV, pooled organ tissue from a nestling budgerigar, purified viral DNA from a l[r] ...
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Polymerase chain reaction assay for detection of human cytomegalovirus
... We tested the PCR assay on urine specimens from patients who had undergone renal transplantation that had been screened for the presence of HCMV by enzyme-linked immunosorbent assay, hyb[r] ...
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hepatitis using the polymerase chain reaction assay (hepadnavirus/hybridization)
... In our study, the sera of 9 chronic hepatitis patients positive for HBsAg, anti-HBc, and anti-HBe were analyzed for the presence of HJ3V DNA by the slot-blot hybridization.. and PCR tech[r] ...
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A Diagnostic Multiplex Polymerase Chain Reaction Assay for Ocular Herpetic Infections
... Results indicate that m-PCR is a more reliable method in rapid diagnosis of herpes viruses DNA in clinical samples than virus isolation.. Key words: multiplex polymera[r] ...
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Polymerase chain reaction assay of parvovirus B19 DNA in clinical specimens
... The serum sample giving a positive reaction (num- ber 37) was tested by dot hybridization and was found to be. positive[r] ...
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Real-time quantitative PCR (polymerase chain reaction)- page 47
... Fig.28C: Relative fold change of CRBN mRNA level in peripheral blood (PB) mononuclear cells of female patient with with del(5q) and trisomy 8 in different clones during len[r] ...