Quantitative real-time PCR (qPCR)
Development and Validation of a Highly Accurate Quantitative Real Time PCR Assay for Diagnosis of Bacterial Vaginosis
... analyzed quantitative real-time PCR (qPCR) assays on DNA extracted from these speci- mens to quantify nine organisms associated with vaginal health or disease: Gardnerella vaginalis, ...
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Quantitative real-time PCR (qPCR) for Eimeria tenella replication – implications for experimental refinement and animal welfare
... chickens. Quantitative real-time PCR test results from tissues collect- ed fi ve dpi indicated a dose-dependent relationship between the size of inoculum and intracellular parasite genome copy ...
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miR-429 suppresses cell proliferation, migration and invasion in nasopharyngeal carcinoma by downregulation of TLN1
... and quantitative real-time PCR (qPCR) were performed to examine TLN1 levels, and qPCR was used to determine miR-429 levels in NPC cell lines with different metastatic ...
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Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites
... potential quantitative characteristics in comparison with conventional diagnostic methods but are often still not routinely ...by quantitative nucleic acid sequence-based amplification (QT-NASBA), ...
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Pneumococcal Colonization Rates in Patients Admitted to a United Kingdom Hospital with Lower Respiratory Tract Infection: a Prospective Case Control Study
... by quantitative real-time PCR (qPCR) targeting lytA might be used to identify the pneumococcus as the etiological pathogen in adults with pneumonia (1) and might be useful as a disease ...
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Aspergillus Specific Lateral Flow Device and Real Time PCR Testing of Bronchoalveolar Lavage Fluid: a Combination Biomarker Approach for Clinical Diagnosis of Invasive Pulmonary Aspergillosis
... (LFD), quantitative real-time PCR (qPCR), and the galactomannan (GM) test were used with 32 BAL fluid samples from 32 patients at risk of ...of qPCR and LFD were 100% and the ...
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Differences in gut microbiota composition between obese and lean children: a cross-sectional study
... and quantitative real-time PCR (qPCR) in order to determine the concentrations of bacterial species belonging to the genera: Bacteroides, Bifidobacterium, Clostridium, Staphylococcus ...
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SNP based real time pyrosequencing as a sensitive and specific tool for identification and differentiation of Rickettsia species in Ixodes ricinus ticks
... on PCR with genus-specific primers to include different occurring Rickettsia species, subsequent species identification is mainly achieved by Sanger ...a real-time pyrosequencing approach was ...
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Comparison of Real Time PCR Assays for Detection of Pathogenic Leptospira spp in Blood and Identification of Variations in Target Sequences
... several real-time PCR assays have been described, and these assays are now used in many diagnostic and reference laboratories for the detection of leptospires in biological fluids of ...patients. ...
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Frequent Copy Gain of the MET Gene in Hypopharyngeal and Laryngeal Cancer in the Japanese Population
... digital PCR (ddPCR) and quantitative real time PCR (qPCR) using 2 distinct fragments of the gene, and mutation was ex- amined in exons 14 - 19 of MET by Sanger ...and qPCR ...
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Use of FTA Cards To Transport Throat Swabs and Oral Fluid Samples for Molecular Detection and Genotyping of Measles and Rubella Viruses
... transcription-quantitative real-time PCR [RT-qPCR]) and genotyping (endpoint RT-PCR) were compared for samples from 238 suspected cases; these samples were either transported ...
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A qPCR method to quantify bioavailable phosphorus using indigenous aquatic species
... a quantitative real-time PCR (qPCR)-based bioas- say was developed to assess phosphorus bioavailability in the water by measuring the transcriptional activity of alkaline phosphatase ...
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Quantitative PCR Assessments of Bacterial Species in Women with and without Bacterial Vaginosis
... broad-range PCR surveys, which show that there are scores of species and genera com- mon to the vaginal environment that had not been recognized, and many of these represent new, as-yet-uncultivated species (18, ...
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Detection of toxoplasmosis in hemodialysis Egyptian patients using serological and molecular techniques
... by quantitative real time PCR for amplification of ...and PCR confirmed the quantitative parasitic load in the peripheral blood in three cases, which indicates that patients on ...
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Research Progress on Reference Genes of Insect for Quantitative Real time Reverse Transcription PCR (RT qPCR)
... Abstract Quantitative real-time reverse transcription PCR (RT-qPCR) has become the most important method for the quantification of mRNA transcription levels owing to its, specificity, ...
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Original Article Expression of IARS2 gene in colon cancer and effect of its knockdown on biological behavior of RKO cells
... Methods: Real-time, fluorescence-based quantitative PCR (qPCR) was used to detect the expression of IARS2 gene in human colon cancer and surrounding tissues and in various cell strains ...
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Hepatitis E Virus (HEV) Detection and Quantification by a Real Time Reverse Transcription PCR Assay Calibrated to the World Health Organization Standard for HEV RNA
... A real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RNA internal control for the simultaneous detection and quantification of HEV ...
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In Situ Analyses Directly in Diarrheal Stool Reveal Large Variations in Bacterial Load and Active Toxin Expression of Enterotoxigenic Escherichia coli and Vibrio cholerae
... culture, real-time quantitative PCR (qPCR) of the toxin profiles, and full- genome sequencing, directly in the diarrheal stool sample as well as in cultured isolated bacterial strains, ...
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Ontogenetic changes in photoreceptor opsin gene expression in coho salmon (Oncorhynchus kisutch, Walbaum)
... 10 pmol of each primer, 2 μ l of cDNA diluted 20-fold, and 1.0 U Platinum Taq DNA polymerase (Invitrogen). DNA amplification was carried out using an MX4000 real-time quantitative PCR system ...
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Characterisation of an efficient atrazine-degrading bacterium, Arthrobacter sp. ZXY-2: an attempt to lay the foundation for potential bioaugmentation applications
... utilised real-time quantitative PCR (RT-qPCR) analysis to measure the transcriptional expression of three atrazine-degrading genes throughout the entire degradation ...
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