quantitative reverse transcription PCR
Kinetics of Poliovirus Shedding following Oral Vaccination as Measured by Quantitative Reverse Transcription PCR versus Culture
... one-step quantitative reverse transcription-PCR (qRT-PCR) assay for detection of OPV Sabin strains 1, 2, and 3 directly in stool samples with an external con- trol to normalize samples ...
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Comparison of Quantitative Reverse Transcription PCR to Viral Culture for Assessment of Respiratory Syncytial Virus Shedding
... Evaluation of subjects in a challenge study is very different from the evaluation of ill patients. In the setting of natural infection, specimens are rarely handled with precision, and transit times and conditions can be ...
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Development of one step quantitative reverse transcription PCR for the rapid detection of flaviviruses
... The ongoing emergence of flaviviruses poses major pub- lic health concerns worldwide. Techniques used for mo- lecular testing and surveillance of flaviviruses should be able to detect and identify a wide range of ...
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Modulation of Immunity-Related Gene Expression in Small Intestines of Mice by Oral Administration of Lactoferrin
... a quantitative reverse transcription-PCR method for 20 immunity-related genes of antimicrobial proteins, pattern recogni- tion receptors, cytokines, and lymphocyte mobilization-related ...
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<p>lncRNA TUG1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma via Regulating miR-29c-3p/<em>COL1A1</em> Axis</p>
... Figure 3 MicroRNA (MiR)-29c-3p was a target gene of taurine upregulated gene 1 (TUG1) in hepatocellular carcinoma (HCC). (A) Bioinformatics analysis showed the predicted binding site between TUG1 and miR-29c-3p. (B) ...
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PubMedCentral-PMC5376639.pdf
... littermates (Fig. 4L), suggesting that neurogenesis in the cerebrum, which mostly occurs in the embryonic stage, is not disturbed. In contrast, Gfap (an astrocyte activation marker) dramatically increased in several ...
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The Lipopolysaccharide and β-1,3-Glucan Binding Protein Gene Is Upregulated in White Spot Virus-Infected Shrimp (Penaeus stylirostris)
... real-time quantitative reverse transcription-PCR, and we showed that LGBP gene expression in shrimp was upregulated as the WSV infection ...
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Comparative Study of Different Standardization Concepts in Quantitative Competitive Reverse Transcription PCR Assays
... vitro transcription as well as amplification were KY80 and KY78; the latter was biotinylated, corresponding to our detection format (see ...95°C. PCR amplification proceeded with 35 cycles at 95°C for 15 s ...
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Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China
... In the second half of 2017, a new type of goose-origin astrovirus (GoAstV) has been isolated from diseased geese in China (5, 6). In our early study, this disease can cause joint swelling and a clear precipitation of ...
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Hepatitis E Virus (HEV) Detection and Quantification by a Real Time Reverse Transcription PCR Assay Calibrated to the World Health Organization Standard for HEV RNA
... real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RNA internal control for the simultaneous detection and quantification of HEV RNA in ...
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Evaluation of the Abbott LCx HIV 1 RNA Quantitative, a New Assay for Quantitative Determination of Human Immunodeficiency Virus Type 1 RNA
... by reverse transcription- PCR or by nucleic acid sequence-based amplification (NASBA) or signal amplification by branched-DNA (bDNA) ...the quantitative measurements of HIV RNA obtained with ...
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Characterization of the gut microbiota of Papua New Guineans using reverse transcription quantitative PCR
... used quantitative real-time polymerase chain reaction targeting dominant and sub-dominant groups of micro- organisms associated with human gut microbiome in 115 people living a subsistence life- style in rural ...
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Upregulation of inflammatory genes in the nasal mucosa of patients undergoing endonasal dacryocystorhinostomy
... controls. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed for the inflammatory genes interleukin (IL)-6, IL-1β, and CCL2, and because of a clear trend of ...
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Developing a Sustainable Insect Pest Management System by Manipulating Plant Volatile Emissions
... for reverse transcription with iScript Reverse Transcription Supermix (Bio-Rad Laboratories, Mississauga, ...ON). Quantitative PCR (qPCR) experiments were run using SsoFast™ ...
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Quantitative assessment of the effect of uracil DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription PCR
... ing PCR, deoxyuridine triphosphate (dUTP) can be substi- tuted for deoxythymidine triphosphate (dTTP) in the synthesis of product ...the PCR reac- ...commercial PCR and RT-PCR reagents to ...
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Quantitation of hepatitis C virus genome molecules in plasma samples
... A competitive reverse transcription PCR cRT-PCR-based assay for the quantitative detection of hepatitis C virus HCV viremia was developed, optimized, and applied to the direct molecular [r] ...
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Use of FTA Cards To Transport Throat Swabs and Oral Fluid Samples for Molecular Detection and Genotyping of Measles and Rubella Viruses
... by reverse transcription-quantitative real-time PCR (RT-qPCR) or the success of genotyping of MeV from samples stored on FTA cards compared to that for TS or oral fluid (OF) samples that were ...
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Quantitative Competitive Reverse Transcription PCR for Quantification of Dengue Virus RNA
... QC-RT- PCR has been successfully utilized for other viruses, such as human immunodeficiency virus type 1 and HCV, and this method has been shown to be useful for assessing both clinical status and response to ...
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Automated quantitative determination of hepatitis C virus viremia by reverse transcription PCR
... HCV RNA was extracted from one HCVpositive serum sample with an unknown viral copy number, reverse transcribed into cDNA, and amplified five times along with an increasing number of mimi[r] ...
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Reference genes for reverse transcription quantitative PCR in canine brain tissue
... of quantitative real-time PCR experiments precise guidelines, the expression of ten frequently used reference genes, namely YWHAZ, HMBS, B2M, SDHA, GAPDH, HPRT, RPL13A, RPS5, RPS19 and GUSB was evaluated in ...
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