Rapid detection and differentiation
Loop Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species
... Rapid detection and differentiation of Taenia species are required for the control and prevention of taeniasis and cysticercosis in areas where these diseases are ...the differentiation of ...
7
Rapid Detection and Differentiation of Clinically Relevant Candida Species Simultaneously from Blood Culture by Use of a Novel Signal Amplification Approach
... fungal detection take several days, which is often too late to impact ...the rapid detection and differentiation of major clinically relevant Candida species directly from blood ...of ...
10
Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... the rapid detection and differentiation of dengue virus serotypes are ...and rapid, wherein the amplification can be obtained in 30 min under isothermal conditions at 63°C by employing a set ...
9
Reverse Transcription PCR Enzyme Linked Immunosorbent Assay for Rapid Detection and Differentiation of Alphavirus Infections
... To overcome some of these diagnostic limitations, we devel- oped an assay that is based on the detection of alphaviral RNA by RT-PCR using two degenerate, genus-specific primers de- veloped previously (16). ...
9
Evaluation of FRET real time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
... The sensitivity, inter- and intra-assay variabilities for four independent experiments are shown in Figure 3A. Positive signals (Ct values) were found for all dilutions except for 0.25 parasite per reaction. A ...
11
Rapid Detection and Differentiation of Antibodies to HIV-1 and HIV-2 Using Multivalent Antigens and Magnetic Immunochromatography Testing
... the detection of antibodies to HIV using magnetic-bead conjugates and multibranched peptides from both HIV-1 and HIV-2 was ...a rapid, low-cost method of determining HIV antibody status requiring no ...
6
Rapid Detection and Molecular Differentiation of Toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans Strains by LightCycler PCR
... The differences in C. diphtheriae and C. ulcerans tox allow a differentiation between these two related virulence genes by PCR (22). For the diagnostic workup of a patient with diph- therialike symptoms, however, ...
5
Rapid Detection and Statistical Differentiation of KPC Gene Variants in Gram Negative Pathogens by Use of High Resolution Melting and ScreenClust Analyses
... In the United States, the production of the Klebsiella pneumoniae carbapenemase (KPC) is an important mechanism of carbap- enem resistance in Gram-negative pathogens. Infections with KPC-producing organisms are ...
5
Reverse transcription multiplex PCR for differentiation between polio and enteroviruses from clinical and environmental samples
... the rapid detection of polioviruses and their differentiation from nonpoliovirus enteroviruses, we developed a protocol in which clinical or environmental specimens are first inoculated onto cell ...
6
Differentiation of Mycobacterial Species by PCR Restriction Analysis of DNA (342 Base Pairs) of the RNA Polymerase Gene (rpoB)
... the differentiation of culture isolates but also for the rapid detection and identification of pathogenic mycobacteria in primary clinical ...
8
Clinical Validation of the Molecular BD GeneOhm StaphSR Assay for Direct Detection of Staphylococcus aureus and Methicillin Resistant Staphylococcus aureus in Positive Blood Cultures
... The rapid detection of Staphylococcus aureus bacteremia and a swift determination of methicillin susceptibility has serious clinical implications affecting patient ...and differentiation of ...
6
Electrically induced bacterial membrane potential dynamics correspond to cellular proliferation capacity
... for rapid detection of proliferative cells in a mixed- species ...offer rapid single-cell level detection of proliferative cells of biological ...the rapid differentiation of ...
6
A rapid peak detection algorithm
... Figure 1 shows the peak detection accuracy of proposed method reach 100%. Comparing to original signals, all peaks and troughs detected by proposed method remain same shape and width. It is because of absence of ...
5
Impact of Early Detection of Respiratory Viruses by Multiplex PCR Assay on Clinical Outcomes in Adult Patients
... A significant drawback of the rapid antigen test is its low sen- sitivity and high discordance with NAAT. In the present study, 82% of patients testing positive for influenza virus had discordant test results when ...
8
Evaluation of novel rapid detection kits for dengue virus NS1 antigen in Dhaka, Bangladesh, in 2017
... Aliquots (30 μL) of serially diluted recombinant NS1 proteins, serially diluted virus stock, or undiluted patient sera were mixed with 60 μL of dilution buffer. The chro- matographic dipsticks then were inserted into ...
15
Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription polymerase chain reaction
... simultaneous detection and differentiation of the three genotypes of ...specific detection of ...crops. Detection limits of the plasmids for multiplex PCR were 100 fg for BrYV-A and BrYV-B, 10 ...
7
Evaluation of an immunochromatography rapid diagnosis kit for detection of chikungunya virus antigen in India, a dengue endemic country
... The detection limit of the IC kit was determined using serially diluted CHIKV recovered from cell culture supernatants; the limit was approximately 10 4 ...of detection of the immunochromatography ...
6
Rapid Differentiation of Closely Related Citrus Genotypes by Fluorescence Spectroscopy
... morphological standpoint, the TSKMA and TSKTR selections are easier to differentiate between themselves and between them and their respective source varieties, TSKC and TSKFL, due to having a greater number of seeds and ...
13
Rapid differentiation of bacterial meningitides by direct gas liquid chromatography
... Rapid identification of Haemophilus influenzae and other bacillary meningitides was attempted by gas-liquid chromatography (GLC) of the metabolic by-products in broth cultures and in cerebrospinal fluid (CSF) ...
7
Rapid patterning and zonal differentiation in a two dimensional Dictyostelium cell mass: the role of pH and ammonia
... TCA-cycle intermediates and related metabolites (Kelly et al., 1979) and ammonia (Schindler and Sussman, 1977) are released from the cells. Under 2-D culturing conditions, outward proton pumping by the plasma membrane ...
9